LOCUS       pZmUBI-A3B-intron-nCas9-3xSV40-NLS-G7T       11918 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...9t-en4mbcm
VERSION     urn.local...9t-en4mbcm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             899..1606
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:93-dgycryy"">mCherry</a>"
                     /label="mCherry"
     Linker          1610..1657
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:.:6j-dgycryx"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             1658..1906
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             join(4200..4484,4673..4964)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A3Bintron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; APOBEC3B (<a
                     href=""urn:local:thjac:1m-ecofc7l"">A3Bintron</a>)"
                     /label="APOBEC3B"
     intron          4486..4674
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="StLS1 Intron"
                     /Transferred_Similarity="95.24%"
                     /Primary_Match="95.24%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:9i-dgycryz"">StLS1 Intron</a>"
                     /label="StLS1 intron"
     Linker          4965..5012
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; XTEN (<a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>)"
                     /label="XTEN"
     CDS             5019..9122
                     /X-Transferred_From="Cas9PTA"
                     /X-Transferred_Similarity="99.98%"
                     /X-Primary_Match="99.98%; Adjusted-Interval-0:0/0; CAS9
                     (<a href=""urn:local:thjac:3c-c4nnd56"">Cas9PTA</a>)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nCAS9(D10A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:pe-ecpo7bv"">nCAS9(D10A)</a>"
                     /label="nCAS9(D10A)"
     NLS             9129..9215
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3xSV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:5q-ecucp4e"">3xSV40</a>"
                     /modified_by="mamee"
                     /label="3xSV40"
     terminator      9223..9494
                     /label="G7T"
     Linker          9499..9547
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(9608..9628)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        9689..9739
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    9740..10549
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      10696..11284
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11883..11903
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacaa
     4201 tggagatcct gagatacctg atggaccccg acaccttcac cttcaatttc aacaacgacc
     4261 ctctggtgct gcggcggaga cagacctacc tgtgttacga ggtggaacgg ctggacaacg
     4321 gcacctgggt tctgatggat cagcacatgg gctttctgtg caacgaggcc aagaacctgc
     4381 tgtgcggctt ctatggcaga cacgccgagc tgcggtttct ggatctggtg ccttctctgc
     4441 agctggaccc cgctcagatc tacagagtga cctggttcat caggtaagtt tctgcttcta
     4501 cctttgatat atatataata attatcatta attagtagta atataatatt tcaaatattt
     4561 ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta
     4621 tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc agctggtccc
     4681 catgcttctc ttggggctgt gctggcgaag tgcgggcctt cctgcaagag aatactcacg
     4741 tgcggctgcg gatcttcgcc gccagaatct acgactacga tcccctgtac aaagaggccc
     4801 tgcagatgct gagagatgcc ggtgctcagg tgtccatcat gacctacgac gagttcgagt
     4861 actgctggga caccttcgtg tacagacagg gctgcccttt ccagccttgg gacggactgg
     4921 aagaacactc tcaggctctg agcggcagac tgagagccat tctttctggt tctgaaactc
     4981 ctggaacttc agaatctgct actcctgaat ccggctcgat ggataagaag tactctatcg
     5041 gactcgctat cggaactaac tctgtgggat gggctgtgat caccgatgag tacaaggtgc
     5101 catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag aaaaacctta
     5161 tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc aagagaaccg
     5221 ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag atcttctcta
     5281 acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca ttcctcgtgg
     5341 aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat gaggtggcat
     5401 accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat tctactgata
     5461 aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc agaggacact
     5521 tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg ttcatccagc
     5581 tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca ggtgtggatg
     5641 ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac ctcattgctc
     5701 agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc tctctcggac
     5761 tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc cagctctcaa
     5821 aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat cagtacgctg
     5881 atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat atcctcagag
     5941 tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga tacgatgagc
     6001 accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca gagaagtaca
     6061 aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat ggtggtgcat
     6121 ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat ggaaccgagg
     6181 aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc ttcgataacg
     6241 gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga aggcaagagg
     6301 atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc accttcagaa
     6361 tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg atgaccagaa
     6421 agtctgagga aaccatcacc ccttggaact tcgaagaggt ggtggataag ggtgctagtg
     6481 ctcagtcttt catcgagagg atgaccaact tcgataagaa ccttccaaac gagaaggtgc
     6541 tccctaagca ctctttgctc tacgagtact tcaccgtgta caacgagttg accaaggtta
     6601 agtacgtgac cgagggaatg aggaagcctg cttttttgtc aggtgagcaa aagaaggcta
     6661 tcgttgatct cttgttcaag accaacagaa aggtgaccgt gaagcagctc aaagaggatt
     6721 acttcaagaa aatcgagtgc ttcgattcag ttgagatttc tggtgttgag gataggttca
     6781 acgcatctct cggaacctac cacgatctcc tcaagatcat taaggataag gatttcttgg
     6841 ataacgagga aaacgaggat atcttggagg atatcgttct taccctcacc ctctttgaag
     6901 atagagagat gattgaagaa aggctcaaga cctacgctca tctcttcgat gataaggtga
     6961 tgaagcagtt gaagagaaga agatacactg gttggggaag gctctcaaga aagctcatta
     7021 acggaatcag ggataagcag tctggaaaga caatccttga tttcctcaag tctgatggat
     7081 tcgctaacag aaacttcatg cagctcatcc acgatgattc tctcaccttt aaagaggata
     7141 tccagaaggc tcaggtttca ggacagggtg atagtctcca tgagcatatc gctaacctcg
     7201 ctggatctcc tgcaatcaag aagggaatcc tccagactgt gaaggttgtg gatgagttgg
     7261 tgaaggtgat gggaaggcat aagcctgaga acatcgtgat cgaaatggct agagagaacc
     7321 agaccactca gaagggacag aagaactcta gggaaaggat gaagaggatc gaggaaggta
     7381 tcaaagagct tggatctcag atcctcaaag agcaccctgt tgagaacact cagctccaga
     7441 atgagaagct ctacctctac tacctccaga acggaaggga tatgtatgtg gatcaagagt
     7501 tggatatcaa caggctctct gattacgatg ttgatcatat cgtgccacag tcattcttga
     7561 aggatgattc tatcgataac aaggtgctca ccaggtctga taagaacagg ggtaagagtg
     7621 ataacgtgcc aagtgaagag gttgtgaaga aaatgaagaa ctattggagg cagctcctca
     7681 acgctaagct catcactcag agaaagttcg ataacttgac taaggctgag aggggaggac
     7741 tctctgaatt ggataaggca ggattcatca agaggcagct tgtggaaacc aggcagatca
     7801 ctaagcacgt tgcacagatc ctcgattcta ggatgaacac caagtacgat gagaacgata
     7861 agttgatcag ggaagtgaag gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa
     7921 aggatttcca attctacaag gtgagggaaa tcaacaacta ccaccacgct cacgatgctt
     7981 accttaacgc tgttgttgga accgctctca tcaagaagta tcctaagctc gagtcagagt
     8041 tcgtgtacgg tgattacaag gtgtacgatg tgaggaagat gatcgctaag tctgagcaag
     8101 agatcggaaa ggctaccgct aagtatttct tctactctaa catcatgaat ttcttcaaga
     8161 ccgagattac cctcgctaac ggtgagatca gaaagaggcc actcatcgag acaaacggtg
     8221 aaacaggtga gatcgtgtgg gataagggaa gggatttcgc taccgttaga aaggtgctct
     8281 ctatgccaca ggtgaacatc gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag
     8341 agtctatcct ccctaagagg aactctgata agctcattgc taggaagaag gattgggacc
     8401 ctaagaaata cggtggtttc gattctccta ccgtggctta ctctgttctc gttgtggcta
     8461 aggttgagaa gggaaagagt aagaagctca agtctgttaa ggaacttctc ggaatcacta
     8521 tcatggaaag gtcatctttc gagaagaacc caatcgattt cctcgaggct aagggataca
     8581 aagaggttaa gaaggatctc atcatcaagc tcccaaagta ctcactcttc gaactcgaga
     8641 acggtagaaa gaggatgctc gcttctgctg gtgagcttca aaagggaaac gagcttgctc
     8701 tcccatctaa gtacgttaac tttctttacc tcgcttctca ctacgagaag ttgaagggat
     8761 ctccagaaga taacgagcag aagcaacttt tcgttgagca gcacaagcac tacttggatg
     8821 agatcatcga gcagatctct gagttctcta aaagggtgat cctcgctgat gcaaacctcg
     8881 ataaggtgtt gtctgcttac aacaagcaca gagataagcc tatcagggaa caggcagaga
     8941 acatcatcca tctcttcacc cttaccaacc tcggtgctcc tgctgctttc aagtacttcg
     9001 atacaaccat cgataggaag agatacacct ctaccaaaga agtgctcgat gctaccctca
     9061 tccatcagtc tatcactgga ctctacgaga ctaggatcga tctctcacag ctcggtggtg
     9121 attcaggtcc aaagaagaag cggaaggtgt ctggaggttc tcctaagaaa aagagaaaag
     9181 tgtccggcgg ctccccgaag aagaagcgca aggtgtaact gcccccgatg agctaagcta
     9241 gctatatcat caatttatgt attacacata atatcgcact cagtctttca tctacggcaa
     9301 tgtaccagct gatataatca gttattgaaa tatttctgaa tttaaacttg catcaataaa
     9361 tttatgtttt tgcttggact ataatacctg acttgttatt ttatcaataa atatttaaac
     9421 tatatttctt tcaagatggg aattaacatc tacaaattgc cttttcttat cgaccatgta
     9481 ctcgacggcc gagtactata agctaggtag taactagtgc gactgtctag aatcgcgtat
     9541 gcgagtgtat ctagaatcgc gtatgcgagt gtatgagctc gatatcccct atagtgagtc
     9601 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     9661 ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa
     9721 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     9781 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     9841 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     9901 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     9961 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
    10021 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
    10081 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
    10141 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
    10201 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
    10261 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
    10321 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
    10381 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
    10441 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
    10501 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
    10561 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
    10621 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
    10681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    11101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    11401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    11461 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11521 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
    11581 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
    11641 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
    11701 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
    11761 gacaaacaac agataaaacg aaaggcccag acttccgact gagcctttcg ttttatttga
    11821 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
    11881 ttgtaaaacg acggccagtc ttaagctcgg gcccacct
//
