LOCUS       pGG-A-gRNA-FLARE-Cas9-scaffold-B        3313 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...9b-en4mbch
VERSION     urn.local...9b-en4mbch
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..536
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaU3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pTaU3
                     (<a href=""urn:local:.:6x-dgycryx"">TaU3</a>)"
                     /label="pTaU3"
     gRNA            536..555
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CBE_Cas9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:32i-eczixuz"">CBE_Cas9</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:hg-ecphww2"">GFP FLARE(T63S)</a>)"
                     /label
     Scaffold        556..638
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:thjac:2f-c4nnd55"">SpCas9
                     Scaffold</a>)"
                     /label="Scaffold"
     GG              complement(639..649)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(669..689)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1169..1273
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1274..2134
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2305..2893
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3257..3278
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tcatgaatcc aaaccacacg gagttcaaat tcccacagat taaggctcgt
       61 ccgtcgcaca aggtaatgtg tgaatattat atctgtcgtg caaaattgcc tggcctgcac
      121 aattgctgtt atagttggcg gcagggagag ttttaacatt gactagcgtg ctgataattt
      181 gtgagaaata ataattgaca agtagatact gacatttgag aagagcttct gaactgttat
      241 tagtaacaaa aatggaaagc tgatgcacgg aaaaaggaaa gaaaaagcca tacttttttt
      301 taggtaggaa aagaaaaagc catacgagac tgatgtctct cagatgggcc gggatctgtc
      361 tatctagcag gcagcagccc accaacctca cgggccagca attacgagtc cttctaaaag
      421 ctcccgccga ggggcgctgg cgctgctgtg cagcagcacg tctaacatta gtcccacctc
      481 gccagtttac agggagcaga accagcttat aagcggaggc gcggcaccaa gaagcactca
      541 cggggtgcag tgcttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      601 tcaacttgaa aaagtggcac cgagtcggtg ctttttttaa catgagaccg aattctcgcc
      661 ctatagtgag tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      721 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      781 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
      841 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
      901 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
      961 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     1021 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     1081 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1501 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1981 tgagcgtggc tctcgaggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3001 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3061 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3241 tatgttgtgt ggaattgtga gcggataaca atttcacacg agctatttag gtgacactat
     3301 agaagtgaag ctt
//
