LOCUS       pGG-AG-PIBT3-KmR_pUM140_0016-UM011_0103_alt-YFP-NOST        8833 bp    DNA     circular UNA 20-APR-2023
DEFINITION  .
ACCESSION   urn.local...1a-eh91nmm
VERSION     urn.local...1a-eh91nmm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1934
                     /modified_by="mamee"
                     /label="pUM140_0016"
     CDS             join(1969..2066,2149..2362,2557..2633,2707..3022,
                     3290..3628)
                     /created_by="jonasblomme"
                     /modified_by="jonasblomme"
                     /label
     CDS             2096..2098
                     /created_by="jonasblomme"
                     /label="alt start"
     CDS             3635..4354
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      4359..4626
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /label="tNOS"
     Linker          4631..4679
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     promoter        4692..5237
                     /label="Ulva prbcS"
     CDS             join(5238..5245,5743..6109)
                     /modified_by="mamee"
                     /label="ble CDS"
     intron          5246..5742
                     /created_by="mamee"
                     /label="RbcsI"
     terminator      6110..6518
                     /label="Ulva trbcS"
     primer          complement(6523..6543)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        6604..6654
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    6655..7464
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7611..8199
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          8798..8818
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
       61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
      121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
      181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
      241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
      301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
      361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
      421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
      481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
      541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
      601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
      661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
      721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
      781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
      841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
      901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
      961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
     1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
     1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
     1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
     1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
     1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
     1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
     1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
     1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
     1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
     1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
     1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
     1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
     1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
     1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
     1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
     1921 atcagtgatt aacaaacagt attcagtcga ctggtaccaa caggctccat gtacctctac
     1981 gcagcactga tcgtcgtcat cttagcgata tgggcagttg cgtcactgcg atggcaagga
     2041 gggcacgagc tgtcgccact cttcatgtga gccttccata gctcgtcgtg tgctgatgca
     2101 attagcttct gcaagtggta gaccacgcca agccgtctta gcgtgcagag ttggccctag
     2161 tgggtgttca gacattgagg acttggccgt ccgtgcacat cgcatatcct tctggccgcc
     2221 ctatccagcg gcaagctgtg atcatcatgg gcacgccgat ccatcgcagc gtagaattgc
     2281 gtcaaagatg gcgcagttga atgcgacaca tgcggagtgc cggttgctgc cggagtcgtt
     2341 ggaatggtat tggaactcaa cagtgcgtcc gccttcgcca tacaatctgc ctttcaaagc
     2401 tgcatgatga tgatcaaagc tcttaacggg actcacctcg cttttccggc gatgctctct
     2461 gctcctgttg ccttgaaacg gctgcaccac agaagtgccg caatatgctc acacgaccac
     2521 tgacacactc ggtttcagcg gggcttgtgc atgcaggccg gcaagtgtgc accctcccgc
     2581 ctgggcaggg agcaagctgc tgcgcttctc cagtgcaaaa ccctcacatt cataggtaag
     2641 acacagccca ccaccggtgc ctctccattc gacacagccg caccagtgat cattcccatc
     2701 gcatgcaggt gacagcatcg tccgcaactt ggcacttgca gcggctgcac tgctggcgga
     2761 acccaacgat ccgcccatca cagtcgaatg gcacgcagac atggtgttct tcagcggttg
     2821 cagcaccaac atcaccttcc tctggcgccc ctttctcgcc aatgtcaccg acgccttgtc
     2881 gacgtggacc ccgccggcag gccagcaccc gcaccacatc atcaccgggg ttgctctgtg
     2941 ggacgttctg cacagcacgg gcatgcatcg atacgcagcc gacgctgatg cgtttgtgga
     3001 tgcggccacc ccgctcctga acgtgcgcac tgcccccgtg caacgacacc ctcttccaga
     3061 gtgtttcata gctgtgactc attagcgcat gatgtcctaa actgtgtcca cttagcatgc
     3121 attgtgcgtg catccttgcg tcccctgcct gcagcacacc atgcaccgag ctcgctgctg
     3181 cgatgtgcgc cttgcagccc catacgcaag cgtacacccc caggcacgct ggtccaaccg
     3241 cggggtggag gcgctgtcag cagcagtcat ggcaggcccc ttcgtacagg cacaagatca
     3301 ggatcgtgca atgtggtggg tgaccacaac ggccgtgcac caggcgcggc tgcagacggc
     3361 cgagaagcgc gcgacgttga cacaggtgaa tctgcagcgg taccgcgctg cgcagcgcat
     3421 gaaggtgcag cagcctctgg cgcacagcac tgcattccat gtgctggacg tgcacgctgt
     3481 cacgcggcag gcgccggcgg cggcgacgga ggatggaatt cactttaacg ccgtggtgta
     3541 tgaggcggca gtgcaggtgc tgctcaacat ggtgcaggcg gaggcgggcg gccgcgctgg
     3601 cgatcggcag gcgtgcacct gtgcgccgtc agctatggtg agcaagggcg aggagctgtt
     3661 cacgggcgtg gtgcccatcc tggtggagct ggacggcgac gtgaacggcc acaagttcag
     3721 cgtgtccggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga agttcatctg
     3781 cacgaccggc aagctgcccg tgccctggcc caccctggtg accaccttcg gctacggcct
     3841 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agagcgccat
     3901 gccggagggc tacgtgcagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3961 ccgcgcggag gtgaagttcg agggcgacac gctggtgaac cgcatcgagc tgaagggcat
     4021 cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca
     4081 caacgtgtac atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4141 ccacaacatc gaggacggct cggtgcagct ggcggaccac taccagcaga acacccccat
     4201 cggcgacggc ccggtgctgc tgcccgacaa ccactacctg tcctaccaga gcgccctgtc
     4261 caaggacccc aacgagaagc gcgaccacat ggtgctgctg gagttcgtga ccgccgccgg
     4321 catcaccctg ggcatggacg agctgtacaa gtgactgcct agagtcaagc agatcgttca
     4381 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     4441 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     4501 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     4561 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     4621 gatcgaacta taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt
     4681 atgagctcga tcgttgttcg tacacaagga tgtcgcttgt ggctttttcg ctcggctttc
     4741 caggagggca gtccagatac catgcggccg ccgtgtccat cactcgcgct gcagctgatg
     4801 tgggcagata ctgcactaat ctgccgcacg acgcgtcggc ctgagtcggc ggacaccgga
     4861 ggcttgctgg agtactggca caagtgtgcg tgtgattgcc agaaacagca acccactcac
     4921 tagatatacc gctccaataa tgggttcatg tgcaagctca ctcaataacg aaacgcttca
     4981 ctattgtgca cgtgccgggg ttgcttgggt atggcgtgcc atacgggttc tgcacatgtc
     5041 acccacgtgg gtgtggcacg agatatcgag gtggccatgt aaaaggcatt tgctagaggg
     5101 cagctcgtcc atgccgctcg agcacaccga gtgcgcacca taaatgcagc acatgcctgt
     5161 gcagcctatc tcctgtctct gttacatccc accacctcct cacatatccc cttccaccct
     5221 taaaccgcac agcagacatg gccaagtgcg catcgcgtgt caatgctggc gctgctgacg
     5281 tccgctgcct aaactgcatg aaagctcctg cagtcgcaag tagtcctttt aacttgttgt
     5341 atcggaacaa atggttgtag tctgtgccgt gtgacgatct tgagccgcct ggtcggcagg
     5401 tccaatgtcg gcctggaccc tgcacaccct gcccaccctg ccgctgctca cccgttcacg
     5461 accccacagg tgctcgcagc agtaattgtc gatagatagc aactgtgttc tgactattct
     5521 ttgaccttcc gcatttggga ctgcggcggc acacctacta tccgcaatgc acaccctggt
     5581 gctggtactg cctgcctttc cgcgcgagcc cacaccatgc aggcgcatag taatggactg
     5641 tgattgattt aggcaactgt atgaaacctc ttcatcgtgc cttggttgct tgcgataccc
     5701 tcacgtgagt tcatatgacc aacgctggca cgacccctgc aggatcacca gcgccgttcc
     5761 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     5821 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     5881 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     5941 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     6001 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     6061 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactaag cagtcaggca
     6121 gagtcgttgt tgggtgtatg gttgcacaaa gctagacctc gagctggtcg gttagaaaga
     6181 gatgcttgtg cgactggatg taacaggcac aatccgagga aaatgcaaca gaaacgttat
     6241 gatgtaacat ctgagcacag tcaataaaat tgcatgttgt atgccgcaca caaaagtgtt
     6301 gttaaacatg cgatgtggtt gtcaaatcat tattcgcaca accatggacc gaacctctgg
     6361 cacgctgcat acgctgtatt cattcacatc atgttttcta cttgtcacat gctctgcacc
     6421 aagtaactcc gattagttag gttcgcacat gtcatcggag tggaccactt cgaatcattt
     6481 ggttcgaaga taaaccggat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     6541 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaataata
     6601 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     6661 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     6721 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     6781 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     6841 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     6901 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     6961 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     7021 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     7081 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     7141 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     7201 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     7261 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     7321 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     7381 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     7441 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     7501 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     7561 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7741 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8401 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8461 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     8521 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     8581 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     8641 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     8701 cccagacttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     8761 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     8821 ctcgggccca cct
//
