LOCUS       pGG-C-UM011_0103.1-D        4221 bp    DNA     circular UNA 20-APR-2023
DEFINITION  .
ACCESSION   urn.local...1f-eh91nmo
VERSION     urn.local...1f-eh91nmo
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             join(14..281,476..554,628..941,1209..1546)
                     /created_by="jonasblomme"
                     /modified_by="jonasblomme"
                     /label="UM011_0103"
     GG              complement(1547..1557)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1577..1597)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2077..2181
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2182..3042
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3213..3801
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4165..4186
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgcaat tagcttctgc aagtggtaga ccacgccaag ccgtcttagc
       61 gtgcagagtt ggccctagtg ggtgttcaga cattgaggac ttggccgtcc gtgcacatcg
      121 catatccttc tggccgccct atccagcggc aagctgtgat catcatgggc acgccgatcc
      181 atcgcagcgt agaattgcgt caaagatggc gcagttgaat gcgacacatg cggagtgccg
      241 gttgctgccg gagtcgttgg aatggtattg gaactcaaca gtgcgtccgc cttcgccata
      301 caatctgcct ttcaaagctg catgatgatg atcaaagctc ttaacgggac tcacctcgct
      361 tttccggcga tgctctctgc tcctgttgcc ttgaaacggc tgcaccacag aagtgccgca
      421 atatgctcac acgaccactg acacactcgg tttcagcggg gcttgtgcat gcaggccggc
      481 aagtgtgcac cctcccgcct gggcagggag caagctgctg cgcttctcca gtgcaaaacc
      541 ctcacattca taggtaagac acagcccacc accggtgcct ctccattcga cacagccgca
      601 ccagtgatca ttcccatcgc atgcaggtga cagcatcgtc cgcaacttgg cacttgcagc
      661 ggctgcactg ctggcggaac ccaacgatcc gcccatcaca gtcgaatggc acgcagacat
      721 ggtgttcttc agcggttgca gcaccaacat caccttcctc tggcgcccct ttctcgccaa
      781 tgtcaccgac gccttgtcga cgtggacccc gccggcaggc cagcacccgc accacatcat
      841 caccggggtt gctctgtggg acgttctgca cagcacgggc atgcatcgat acgcagccga
      901 cgctgatgcg tttgtggatg cggccacccc gctcctgaac gtgcgcactg cccccgtgca
      961 acgacaccct cttccagagt gtttcatagc tgtgactcat tagcgcatga tgtcctaaac
     1021 tgtgtccact tagcatgcat tgtgcgtgca tccttgcgtc ccctgcctgc agcacaccat
     1081 gcaccgagct cgctgctgcg atgtgcgcct tgcagcccca tacgcaagcg tacaccccca
     1141 ggcacgctgg tccaaccgcg gggtggaggc gctgtcagca gcagtcatgg caggcccctt
     1201 cgtacaggca caagatcagg atcgtgcaat gtggtgggtg accacaacgg ccgtgcacca
     1261 ggcgcggctg cagacggccg agaagcgcgc gacgttgaca caggtgaatc tgcagcggta
     1321 ccgcgctgcg cagcgcatga aggtgcagca gcctctggcg cacagcactg cattccatgt
     1381 gctggacgtg cacgctgtca cgcggcaggc gccggcggcg gcgacggagg atggaattca
     1441 ctttaacgcc gtggtgtatg aggcggcagt gcaggtgctg ctcaacatgg tgcaggcgga
     1501 ggcgggcggc cgcgctggcg atcggcaggc gtgcacctgt gcgccgtcag tgagaccgaa
     1561 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     1621 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1681 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1741 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     1801 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1861 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1921 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1981 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2041 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2101 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2161 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2221 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2281 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2341 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2401 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2461 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2521 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2581 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2641 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2701 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2761 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2821 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2881 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     2941 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3001 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3061 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3121 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3181 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3241 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3301 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3361 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3421 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3481 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3541 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3601 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3661 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3721 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3781 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3841 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3901 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3961 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4021 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4081 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4141 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     4201 gacactatag aagtgaagct t
//
