LOCUS       pGGC000-TetR            4449 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...5u-ed5nrz0
VERSION     urn.local...5u-ed5nrz0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             complement(18..323)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:t-cj5879l"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(324..642)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(665..1324)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(1378..1408)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     GG              complement(1426..1436)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1456..1476)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     misc_feature    2038..3228
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
                     /modified_by="mamee"
                     /label="TcR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3441..4029
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4393..4414
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tgtcgactta tattccccag aacatcaggt taatggcgtt tttgatgtca
       61 ttttcgcggt ggctgagatc agccacttct tccccgataa cggaaaccgg cacactggcc
      121 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
      181 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
      241 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
      301 taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt
      361 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
      421 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
      481 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
      541 ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat attcttatac
      601 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac
      661 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      721 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      781 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      841 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
      901 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      961 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1021 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1081 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     1141 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1201 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1261 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1321 ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa atccacacat
     1381 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgtcagt gagaccgaat
     1441 tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa
     1501 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1561 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1621 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     1681 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1741 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1801 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1861 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1921 cttagacgtc aggtggcact tttcggggaa atgtgccatg tttgacagct tatcatcgat
     1981 tagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
     2041 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
     2101 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc
     2161 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
     2221 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
     2281 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat tctctacgcc
     2341 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     2401 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     2461 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttacatgca
     2521 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     2581 caggagtcgc ataagggaga gcgccgaccc atgcccttga gagccttcaa cccagtcagc
     2641 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     2701 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     2761 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     2821 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     2881 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     2941 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     3001 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     3061 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     3121 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     3181 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     3241 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     3301 ctgtgaatgc gcaaaccaac ccttgatcgg ggaagaacag tatgtcgagc tattttttga
     3361 cttactgggg atcaagcctg attgggagaa aataaaatat cccctatagt gagtcgtatt
     3421 acatggatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     4381 ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa
     4441 gtgaagctt
//
