LOCUS       pGGC000-GmR             5144 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...5u-ed5nrz0
VERSION     urn.local...5u-ed5nrz0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             complement(18..323)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(324..642)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:thjac:t-cj5879l"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(665..1324)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(1378..1408)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     GG              complement(1426..1436)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1456..1476)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     misc_feature    complement(2717..3250)
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2q-c4nnd56"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4136..4724
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5088..5109
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tgtcgactta tattccccag aacatcaggt taatggcgtt tttgatgtca
       61 ttttcgcggt ggctgagatc agccacttct tccccgataa cggaaaccgg cacactggcc
      121 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
      181 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
      241 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
      301 taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt
      361 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
      421 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
      481 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
      541 ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat attcttatac
      601 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac
      661 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      721 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      781 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      841 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
      901 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      961 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1021 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1081 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     1141 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1201 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1261 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1321 ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa atccacacat
     1381 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgtcagt gagaccgaat
     1441 tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa
     1501 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1561 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1621 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     1681 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1741 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1801 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1861 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1921 cttagacgtc aggtggcact tttcggggaa atgtgccgtg tctcaaaatc tctgatgtta
     1981 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2041 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2101 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2161 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2221 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2281 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2341 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2401 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2461 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2521 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2581 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2641 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaggccggg aagccgatct
     2701 cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt
     2761 cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca
     2821 cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg
     2881 tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac
     2941 tacgcggctg ctcaaacctg ggcagaacgt aagccgcgag agcgccaaca accgcttctt
     3001 ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg
     3061 agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa
     3121 gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca
     3181 tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     3241 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     3301 tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc
     3361 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     3421 atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc
     3481 ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca
     3541 tttctgtcct ggctggcgaa cgagcgcaag gtttcgctct ccacgcatcg tcaggcattg
     3601 gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag
     3661 atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt
     3721 cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt
     3781 tctagtggtt ggctactaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     3841 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     3901 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     3961 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     4021 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttttcgt
     4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc gatcaaagga tcttcttgag
     4141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4801 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4861 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4921 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4981 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5041 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5101 aatttcacac gagctattta ggtgacacta tagaagtgaa gctt
//
