LOCUS       pGG-B-3xHA_TurboID_2xG4S-C        3801 bp    DNA     circular UNA 07-SEP-2022
DEFINITION  .
ACCESSION   p01000
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:87-dgycryy"">B</a>"
                     /label="B"
     RBS             12..43
                     /label="omega leader"
     CDS             44..1093
                     /label="N_TurboID"
     Tag             47..127
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="3xHA"
     Linker          1097..1126
                     /region_type="Connecting peptide"
                     /modified_by="mamee"
                     /label="2xG4S linker"
     GG              complement(1127..1137)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:80-dgycryx"">C</a>"
                     /label="C"
     primer          complement(1157..1177)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1657..1761
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1762..2622
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2793..3381
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3745..3766
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aaacaacaaa caacaaacaa cattacaatt acaatgtacc catacgatgt
       61 tccagattac gcttacccat acgatgttcc agattacgct tacccatacg atgttccaga
      121 ttacgctgcc aaagacaata ccgtgcccct caaactaatt gcattgcttg ccaatggtga
      181 gttccattct ggggaacaac ttggggagac tctgggcatg tcccgagcgg ccattaataa
      241 gcacatacag actttgaggg actggggtgt cgatgtgttt acagtaccgg gtaagggata
      301 ttctctgccg gagccgatac ctttactcaa tgcaaagcag attttaggac agctggatgg
      361 cgggagcgtg gctgtcctcc cggtggtcga tagtacaaat caatatcttt tggacaggat
      421 cggtgagctg aaatctggag atgcttgcat agcggaatac caacaagctg gtaggggatc
      481 tcgaggccga aagtggtttt caccctttgg ggccaactta tacttatcca tgttctggcg
      541 tctgaaacgt ggcccggcag ccatagggct agggcccgta attgggatcg ttatggcgga
      601 agcgttacga aagttgggag ccgataaagt cagagtaaag tggccaaatg atctttattt
      661 acaagataga aagctggcgg gcattcttgt tgagcttgcc ggaataacag gcgatgccgc
      721 acagatcgtt ataggggccg ggattaatgt ggctatgagg agggtcgaag aaagtgttgt
      781 gaatcaagga tggattactc tccaagaggc gggtataaat cttgatcgaa atactctagc
      841 tgcaaccttg atacgagagc taagagcagc actagaattg tttgaacaag aagggctggc
      901 cccatatctc cctcgatggg agaagttgga caattttata aacagaccgg tgaagttaat
      961 cattggagac aaagaaattt tcggtataag ccgtgggatc gataaacagg gtgctcttct
     1021 ccttgagcag gacggtgtca tcaagccttg gatgggaggc gaaatctctc tccgaagcgc
     1081 cgagaagaag cttgacggtg gcggaggttc tggcggtgga ggatccggct tgagaccgaa
     1141 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     1201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1321 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     1381 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1441 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1501 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1561 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1621 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1681 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1741 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1801 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1861 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1921 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1981 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2041 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2101 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2161 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2221 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2281 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2341 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2401 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2461 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     2521 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2581 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2641 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2701 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3421 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3481 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3541 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3601 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3661 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3721 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     3781 gacactatag aagtgaagct t
//
