LOCUS       pG3-U1-AG-U9            4567 bp    DNA     circular UNA 19-FEB-2024
DEFINITION  pLAPAU21.
ACCESSION   urn.local.lapau.2w-dm39m5j
VERSION     urn.local.lapau.2w-dm39m5j
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        354..381
                     /invitrogen=2250000
                     /vntifkey=29
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pDEST32-GG"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pc
                     promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pc (<a
                     href=""urn:local:.:2d-f0u4295"">GmR</a>)"
                     /label="pc"
     misc_feature    570..1103
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="99.81%"
                     /Primary_Match="99.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2q-c4nnd56"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1454..2042
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     rep_origin      2157..2766
                     /created_by="jonas"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriV"
                     /Transferred_Similarity="99.84%"
                     /Primary_Match="99.84%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:27y-hbq6m8l"">oriV</a>"
                     /label="oriV"
     regulatory      complement(2835..2858)
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTiT37 overdrive"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7j-dm4ikhd"">pTiT37 overdrive</a>"
                     /label="pTiT37 overdrive"
     regulatory      complement(2922..2946)
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTiT37 RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7r-dm4iwd5"">pTiT37 RB</a>"
                     /label="pTiT37 RB"
     misc_feature    2958..2997
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7p-dgycryx"">U1</a>"
                     /label="U1"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(2998..3015)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     GG              complement(3016..3026)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2b8-ciz7x4i"">A</a>"
                     /label="A"
     CDS             complement(3033..3338)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:t-cj5879l"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(3339..3657)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:thjac:t-cj5879l"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(3680..4339)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (<a
                     href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(4392..4422)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:thjac:23i-ciywvzs"">CmR</a>)"
                     /Alternative_Match="93.55%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:d-c4nncz3"">pLac</a>"
                     /label="lacUV5"
     GG              4447..4457
                     /%_Identity=100
                     /Motif="GGTCTCAGTAT"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; G
                     overlap (<a href=""urn:local:thjac:2bm-ciz835c"">G</a>)"
                     /label="G overlap"
     misc_difference 4458..4475
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    4476..4515
                     /standard_name="U9"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7x-dgycryx"">U9</a>"
                     /note="Geneious type: Golden Gibson Cloning site"
     regulatory      complement(4543..4567)
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTiT37 LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7n-dm4itu5"">pTiT37 LB</a>"
                     /label="pTiT37 LB"
ORIGIN      
        1 ccagctcgag gcggcgccct gcagccagcc aacagctccc cgaccggcag ctcggcacaa
       61 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
      121 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
      181 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
      241 cgttgctgcc tgtgatcacc gcggtttcaa ccttgtattc accggtgtta tggttaatta
      301 acagcttcaa ctttattata caaagttgat aattcactgg cgcgccgcta gtttgacata
      361 agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      421 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      481 ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      541 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagcaacgat gttacgcagc
      601 agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt cgcacatgta
      661 ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc ggtcgtgagt
      721 tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc gggaacttgc
      781 tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt gttggcgctc
      841 tcgcggctta cgttctgccc aagtttgagc agccgcgtag tgagatctat atctatgatc
      901 tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc aatctcctca
      961 agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat tacggtgacg
     1021 atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg cactttgata
     1081 tcgacccaag taccgccacc taatcaattc gttcaagccg agatcggctt cccggccgcg
     1141 gagttgttcg gtaaattgtc acaacgccgc gaatatagtc tttaccatgc ccttggccac
     1201 gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca
     1261 taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat
     1321 tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt
     1381 tattgtccgc ccacacccgc ttcgccccgt tccactgagc gtcagacccc gtagaaaaga
     1441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1561 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct agatagctgg
     2161 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     2221 cgaagccgtg tgcgagacac cccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg
     2281 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
     2341 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
     2401 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
     2461 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
     2521 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
     2581 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
     2641 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
     2701 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc cttctcgaac
     2761 cctcccggtc gagtcatggt ctagcatgga tctcggggac gtcgtacttt gatcccgagg
     2821 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     2881 tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
     2941 ctgtcaagct tgagctccat tactcgcatc cattctcagg ctgtctcgtc tcgtctctag
     3001 ggataacagg gtaatacctt gagaccgtcg acttatattc cccagaacat caggttaatg
     3061 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag
     3121 actggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
     3181 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
     3241 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
     3301 cggctctctc ttttataggt gtaaacctta aactgcattt caccagtccc tgttctcgtc
     3361 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
     3421 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
     3481 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
     3541 actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta tacatatcag
     3601 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta
     3661 gtaagccgga tccacgcgtt tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     3721 cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca
     3781 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     3841 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     3901 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     3961 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     4021 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     4081 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca
     4141 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     4201 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     4261 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     4321 cagtgatttt tttctccatt ttagcttcct tagctcctga aatctcgacg gatcctaact
     4381 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatgc
     4441 ggccgcggtc tcagtatatt accctgttat ccctagttcc ttatcatctg gcgaatcgga
     4501 cccacaagag cactggggcc ctctagagga tccccgggta ctgtttacac cacaatatat
     4561 cctgcca
//
