LOCUS       pDE-Cas9(VQR)-Km       15584 bp ds-DNA     circular     17-APR-2023
DEFINITION  .
ACCESSION   urn.local...12-ehucfj9
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Anne Britt
  TITLE     Direct Submission
  JOURNAL   Exported 15 sep. 2015 from SnapGene 2.8.2 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|706665600|.
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="99.70%"
                     /Primary_Match="99.70%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer          516..537
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
     promoter        569..1540
                     /vntifkey=29
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pPcUBI"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:76-dgycryx"">pPcUBI</a>"
                     /label="pPcUBI"
     CDS             1555..5694
                     /modified_by="mamee"
                     /label="Cas9"
     NLS             5671..5691
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     terminator      5709..6177
                     /modified_by="mamee"
                     /label="pea 3A term"
     attR1           6204..6327
                     /modified_by="mamee"
     promoter        6352..6382
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacUV5 (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    6436..7094
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="14 CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (<a
                     href=""urn:local:.:1h-fz09kes"">14 CmR</a>)"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     UTR             7117..7435
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     CDS             7436..7741
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /label="ccdB"
     attR2           complement(7782..7905)
                     /modified_by="mamee"
     Plant_Selection 7970..9269
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="95.75%"
                     /Primary_Match="95.75%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     promoter        7970..8149
                     /note="nopaline synthase promoter"
                     /modified_by="mamee"
                     /label="pNOS"
     CDS             8183..8977
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin(R))"
                     /modified_by="mamee"
                     /label="NptII"
     terminator      9017..9269
                     /note="nopaline synthase terminator and poly(A) signal"
                     /modified_by="mamee"
                     /label="tNOS"
     T-DNA_border    9270..9456
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="76.62%"
                     /Primary_Match="76.62%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     regulatory      10612..11241
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     regulatory      11670..12742
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     rep_origin      12808..13002
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      13376..13636
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(13672..14260)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    complement(14504..15295)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     Promoter        complement(15296..15577)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="96.81%"
                     /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgctctagcc aatacgcaaa ccgcctctcc
      361 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      421 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      541 gaaacagcta tgacatgatt acgaattcaa aaattacgga tatgaatata ggcatatccg
      601 tatccgaatt atccgtttga cagctagcaa cgattgtaca attgcttctt taaaaaagga
      661 agaaagaaag aaagaaaaga atcaacatca gcgttaacaa acggccccgt tacggcccaa
      721 acggtcatat agagtaacgg cgttaagcgt tgaaagactc ctatcgaaat acgtaaccgc
      781 aaacgtgtca tagtcagatc ccctcttcct tcaccgcctc aaacacaaaa ataatcttct
      841 acagcctata tatacaaccc ccccttctat ctctcctttc tcacaattca tcatctttct
      901 ttctctaccc ccaattttaa gaaatcctct cttctcctct tcattttcaa ggtaaatctc
      961 tctctctctc tctctctctg ttattccttg ttttaattag gtatgtatta ttgctagttt
     1021 gttaatctgc ttatcttatg tatgccttat gtgaatatct ttatcttgtt catctcatcc
     1081 gtttagaagc tataaatttg ttgatttgac tgtgtatcta cacgtggtta tgtttatatc
     1141 taatcagata tgaatttctt catattgttg cgtttgtgtg taccaatccg aaatcgttga
     1201 tttttttcat ttaatcgtgt agctaattgt acgtatacat atggatctac gtatcaattg
     1261 ttcatctgtt tgtgtttgta tgtatacaga tctgaaaaca tcacttctct catctgattg
     1321 tgttgttaca tacatagata tagatctgtt atatcatttt ttttattaat tgtgtatata
     1381 tatatgtgca tagatctgga ttacatgatt gtgattattt acatgatttt gttatttacg
     1441 tatgtatata tgtagatctg gactttttgg agttgttgac ttgattgtat ttgtgtgtgt
     1501 atatgtgtgt tctgatcttg atatgttatg tatgtgcagc gaattcggcg cgccatggat
     1561 aagaagtact ctatcggact cgatatcgga actaactctg tgggatgggc tgtgatcacc
     1621 gatgagtaca aggtgccatc taagaagttc aaggttctcg gaaacaccga taggcactct
     1681 atcaagaaaa accttatcgg tgctctcctc ttcgattctg gtgaaactgc tgaggctacc
     1741 agactcaaga gaaccgctag aagaaggtac accagaagaa agaacaggat ctgctacctc
     1801 caagagatct tctctaacga gatggctaaa gtggatgatt cattcttcca caggctcgaa
     1861 gagtcattcc tcgtggaaga agataagaag cacgagaggc accctatctt cggaaacatc
     1921 gttgatgagg tggcatacca cgagaagtac cctactatct accacctcag aaagaagctc
     1981 gttgattcta ctgataaggc tgatctcagg ctcatctacc tcgctctcgc tcacatgatc
     2041 aagttcagag gacacttcct catcgagggt gatctcaacc ctgataactc tgatgtggat
     2101 aagttgttca tccagctcgt gcagacctac aaccagcttt tcgaagagaa ccctatcaac
     2161 gcttcaggtg tggatgctaa ggctatcctc tctgctaggc tctctaagtc aagaaggctt
     2221 gagaacctca ttgctcagct ccctggtgag aagaagaacg gacttttcgg aaacttgatc
     2281 gctctctctc tcggactcac ccctaacttc aagtctaact tcgatctcgc tgaggatgca
     2341 aagctccagc tctcaaagga tacctacgat gatgatctcg ataacctcct cgctcagatc
     2401 ggagatcagt acgctgattt gttcctcgct gctaagaacc tctctgatgc tatcctcctc
     2461 agtgatatcc tcagagtgaa caccgagatc accaaggctc cactctcagc ttctatgatc
     2521 aagagatacg atgagcacca ccaggatctc acacttctca aggctcttgt tagacagcag
     2581 ctcccagaga agtacaaaga gattttcttc gatcagtcta agaacggata cgctggttac
     2641 atcgatggtg gtgcatctca agaagagttc tacaagttca tcaagcctat cctcgagaag
     2701 atggatggaa ccgaggaact cctcgtgaag ctcaatagag aggatcttct cagaaagcag
     2761 aggaccttcg ataacggatc tatccctcat cagatccacc tcggagagtt gcacgctatc
     2821 cttagaaggc aagaggattt ctacccattc ctcaaggata acagggaaaa gattgagaag
     2881 attctcacct tcagaatccc ttactacgtg ggacctctcg ctagaggaaa ctcaagattc
     2941 gcttggatga ccagaaagtc tgaggaaacc atcacccctt ggaacttcga agaggtggtg
     3001 gataagggtg ctagtgctca gtctttcatc gagaggatga ccaacttcga taagaacctt
     3061 ccaaacgaga aggtgctccc taagcactct ttgctctacg agtacttcac cgtgtacaac
     3121 gagttgacca aggttaagta cgtgaccgag ggaatgagga agcctgcttt tttgtcaggt
     3181 gagcaaaaga aggctatcgt tgatctcttg ttcaagacca acagaaaggt gaccgtgaag
     3241 cagctcaaag aggattactt caagaaaatc gagtgcttcg attcagttga gatttctggt
     3301 gttgaggata ggttcaacgc atctctcgga acctaccacg atctcctcaa gatcattaag
     3361 gataaggatt tcttggataa cgaggaaaac gaggatatct tggaggatat cgttcttacc
     3421 ctcaccctct ttgaagatag agagatgatt gaagaaaggc tcaagaccta cgctcatctc
     3481 ttcgatgata aggtgatgaa gcagttgaag agaagaagat acactggttg gggaaggctc
     3541 tcaagaaagc tcattaacgg aatcagggat aagcagtctg gaaagacaat ccttgatttc
     3601 ctcaagtctg atggattcgc taacagaaac ttcatgcagc tcatccacga tgattctctc
     3661 acctttaaag aggatatcca gaaggctcag gtttcaggac agggtgatag tctccatgag
     3721 catatcgcta acctcgctgg atctcctgca atcaagaagg gaatcctcca gactgtgaag
     3781 gttgtggatg agttggtgaa ggtgatggga aggcataagc ctgagaacat cgtgatcgaa
     3841 atggctagag agaaccagac cactcagaag ggacagaaga actctaggga aaggatgaag
     3901 aggatcgagg aaggtatcaa agagcttgga tctcagatcc tcaaagagca ccctgttgag
     3961 aacactcagc tccagaatga gaagctctac ctctactacc tccagaacgg aagggatatg
     4021 tatgtggatc aagagttgga tatcaacagg ctctctgatt acgatgttga tcatatcgtg
     4081 ccacagtcat tcttgaagga tgattctatc gataacaagg tgctcaccag gtctgataag
     4141 aacaggggta agagtgataa cgtgccaagt gaagaggttg tgaagaaaat gaagaactat
     4201 tggaggcagc tcctcaacgc taagctcatc actcagagaa agttcgataa cttgactaag
     4261 gctgagaggg gaggactctc tgaattggat aaggcaggat tcatcaagag gcagcttgtg
     4321 gaaaccaggc agatcactaa gcacgttgca cagatcctcg attctaggat gaacaccaag
     4381 tacgatgaga acgataagtt gatcagggaa gtgaaggtta tcaccctcaa gtcaaagctc
     4441 gtgtctgatt tcagaaagga tttccaattc tacaaggtga gggaaatcaa caactaccac
     4501 cacgctcacg atgcttacct taacgctgtt gttggaaccg ctctcatcaa gaagtatcct
     4561 aagctcgagt cagagttcgt gtacggtgat tacaaggtgt acgatgtgag gaagatgatc
     4621 gctaagtctg agcaagagat cggaaaggct accgctaagt atttcttcta ctctaacatc
     4681 atgaatttct tcaagaccga gattaccctc gctaacggtg agatcagaaa gaggccactc
     4741 atcgagacaa acggtgaaac aggtgagatc gtgtgggata agggaaggga tttcgctacc
     4801 gttagaaagg tgctctctat gccacaggtg aacatcgtta agaaaaccga ggtgcagacc
     4861 ggtggattct ctaaagagtc tatcctccct aagaggaact ctgataagct cattgctagg
     4921 aagaaggatt gggaccctaa gaaatacggt ggtttcgtgt ctcctaccgt ggcttactct
     4981 gttctcgttg tggctaaggt tgagaaggga aagagtaaga agctcaagtc tgttaaggaa
     5041 cttctcggaa tcactatcat ggaaaggtca tctttcgaga agaacccaat cgatttcctc
     5101 gaggctaagg gatacaaaga ggttaagaag gatctcatca tcaagctccc aaagtactca
     5161 ctcttcgaac tcgagaacgg tagaaagagg atgctcgctt ctgctggtga gcttcaaaag
     5221 ggaaacgagc ttgctctccc atctaagtac gttaactttc tttacctcgc ttctcactac
     5281 gagaagttga agggatctcc agaagataac gagcagaagc aacttttcgt tgagcagcac
     5341 aagcactact tggatgagat catcgagcag atctctgagt tctctaaaag ggtgatcctc
     5401 gctgatgcaa acctcgataa ggtgttgtct gcttacaaca agcacagaga taagcctatc
     5461 agggaacagg cagagaacat catccatctc ttcaccctta ccaacctcgg tgctcctgct
     5521 gctttcaagt acttcgatac aaccatcgat aggaagcagt acagatctac caaagaagtg
     5581 ctcgatgcta ccctcatcca tcagtctatc actggactct acgagactag gatcgatctc
     5641 tcacagctcg gtggtgattc aagggctgat cctaagaaga agaggaaggt ttgaggcgcg
     5701 ccgagctcca ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg ttcgtcaagt
     5761 tcaatgcatc agtttcattg cccacacacc agaatcctac taagtttgag tattatggca
     5821 ttggaaaagc tgttttcttc tatcatttgt tctgcttgta atttactgtg ttctttcagt
     5881 ttttgttttc ggacatcaaa atgcaaatgg atggataaga gttaataaat gatatggtcc
     5941 ttttgttcat tctcaaatta ttattatctg ttgtttttac tttaatgggt tgaatttaag
     6001 taagaaagga actaacagtg tgatattaag gtgcaatgtt agacatataa aacagtcttt
     6061 cacctctctt tggttatgtc ttgaattggt ttgtttcttc acttatctgt gtaatcaagt
     6121 ttactatgag tctatgatca agtaattatg caatcaagtt aagtacagta taggcttgag
     6181 ctccctaggc ctgttatccc taacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     6241 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     6301 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
     6361 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     6421 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     6481 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     6541 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     6601 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     6661 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     6721 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     6781 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     6841 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     6901 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     6961 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     7021 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     7081 caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt
     7141 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     7201 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     7261 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     7321 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     7381 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     7441 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     7501 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     7561 gtcagataaa gtcccccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     7621 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     7681 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     7741 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     7801 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     7861 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga taattccgat
     7921 ccagcctagg cccgggcctg aggacgcgtc catggttaat taagacgtcg aaccgcaacg
     7981 ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac atacgtcaga
     8041 aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatttcttg
     8101 tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag agtctcatat
     8161 tcactctcaa ctcgatcgag gcatgattga acaagatgga ttgcacgcag gttctccggc
     8221 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     8281 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     8341 gtccggtgcc ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc tggccacgac
     8401 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     8461 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     8521 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8581 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8641 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8701 gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg acccacggcg atgcctgctt
     8761 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8821 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8881 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8941 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcggactc
     9001 tagctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     9061 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     9121 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     9181 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     9241 gcgcgcggtg tcatctatgt tactagatcg tttaaactat cagtgtttga caggatatat
     9301 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     9361 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     9421 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     9481 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     9541 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     9601 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     9661 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     9721 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     9781 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     9841 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     9901 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     9961 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
    10021 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
    10081 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
    10141 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
    10201 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
    10261 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
    10321 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
    10381 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
    10441 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
    10501 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
    10561 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
    10621 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
    10681 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
    10741 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
    10801 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
    10861 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
    10921 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
    10981 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
    11041 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
    11101 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
    11161 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    11221 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    11281 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    11341 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    11401 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    11461 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    11521 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
    11581 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
    11641 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
    11701 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
    11761 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
    11821 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
    11881 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
    11941 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
    12001 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
    12061 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
    12121 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
    12181 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
    12241 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
    12301 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
    12361 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
    12421 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
    12481 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
    12541 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
    12601 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
    12661 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    12721 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    12781 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
    12841 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    12901 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    12961 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    13021 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    13081 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    13141 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    13201 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    13261 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    13321 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    13381 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    13441 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    13501 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    13561 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    13621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    13681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    13741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    13801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    13861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    13921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    13981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    14041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    14101 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    14161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    14221 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    14281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga
    14341 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt
    14401 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac
    14461 gccggcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct
    14521 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
    14581 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
    14641 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
    14701 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
    14761 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
    14821 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
    14881 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
    14941 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
    15001 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
    15061 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
    15121 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
    15181 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
    15241 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt
    15301 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
    15361 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
    15421 caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt accaccgctg
    15481 cgttcggtca aggttctgga ccagttgcgt gacggcagtt acgctacttg cattacagct
    15541 tacgaaccga acgaggctta tgtccactgg gttcgtgccc gaat
//
