LOCUS       pGG-C-FnCas12a-D        6726 bp    DNA     circular UNA 14-APR-2023
DEFINITION  .
ACCESSION   urn.local...l-946qpqi
VERSION     urn.local...l-946qpqi
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..4051
                     /created_by="User"
                     /modified_by="User"
                     /label="hFnCpf1"
     NLS             3914..3961
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="AAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAG"
                     /annotation_group="NLS: 4,829 -> 4,876"
     Linker          3962..3967
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GGATCC"
                     /annotation_group="GS linker: 4,877 -> 4,882"
                     /label="GS linker"
     Tag             3968..4048
                     /%_Identity=100
                     /Motif="TACCCATACGATGTTCCAGATTACGCTTATCCCTACGACGTGCCTGATTA
                     TGCATACCCATATGATGTCCCCGACTATGCC"
                     /annotation_group="3X HA Tag: 4,883 -> 4,963"
                     /modified_by="mamee"
                     /label="3xHA"
     GG              complement(4052..4062)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="D"
     primer          complement(4082..4102)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        4582..4686
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    4687..5547
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5718..6306
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6670..6691
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgagca tctaccagga gttcgtcaac aagtattcac tgagtaagac
       61 actgcggttc gagctgatcc cacagggcaa gacactggag aacatcaagg cccgaggcct
      121 gattctggac gatgagaagc gggcaaaaga ctataagaaa gccaagcaga tcattgataa
      181 ataccaccag ttctttatcg aggaaattct gagctccgtg tgcatcagtg aggatctgct
      241 gcagaattac tcagacgtgt acttcaagct gaagaagagc gacgatgaca acctgcagaa
      301 ggacttcaag tccgccaagg acaccatcaa gaaacagatt agcgagtaca tcaaggactc
      361 cgaaaagttt aaaaatctgt tcaaccagaa tctgatcgat gctaagaaag gccaggagtc
      421 cgacctgatc ctgtggctga aacagtctaa ggacaatggg attgaactgt tcaaggctaa
      481 ctccgatatc actgatattg acgaggcact ggaaatcatc aagagcttca agggatggac
      541 cacatacttt aaaggcttcc acgagaaccg caagaacgtg tactccagca acgacattcc
      601 tacctccatc atctaccgaa tcgtcgatga caatctgcca aagttcctgg agaacaaggc
      661 caaatatgaa tctctgaagg acaaagctcc cgaggcaatt aattacgaac agatcaagaa
      721 agatctggct gaggaactga cattcgatat cgactataag actagcgagg tgaaccagag
      781 ggtcttttcc ctggacgagg tgtttgaaat cgccaatttc aacaattacc tgaaccagtc
      841 cggcattact aaattcaata ccatcattgg cgggaagttt gtgaacgggg agaataccaa
      901 gcgcaaggga attaacgaat acatcaatct gtatagccag cagatcaacg acaaaactct
      961 gaagaaatac aagatgtctg tgctgttcaa acagatcctg agtgataccg agtccaagtc
     1021 ttttgtcatt gataaactgg aagatgactc agacgtggtc actaccatgc agagctttta
     1081 tgagcagatc gccgctttca agacagtgga ggaaaaatct attaaggaaa ctctgagtct
     1141 gctgttcgat gacctgaaag cccagaagct ggacctgagt aagatctact tcaaaaacga
     1201 taagagtctg acagacctgt cacagcaggt gtttgatgac tattccgtga ttgggaccgc
     1261 cgtcctggag tacattacac agcagatcgc tccaaagaac ctggataatc cctctaagaa
     1321 agagcaggaa ctgatcgcta agaaaaccga gaaggcaaaa tatctgagtc tggaaacaat
     1381 taagctggca ctggaggagt tcaacaagca cagggatatt gacaaacagt gccgctttga
     1441 ggaaatcctg gccaacttcg cagccatccc catgattttt gatgagatcg cccagaacaa
     1501 agacaatctg gctcagatca gtattaagta ccagaaccag ggcaagaaag acctgctgca
     1561 ggcttcagca gaagatgacg tgaaagccat caaggatctg ctggaccaga ccaacaatct
     1621 gctgcacaag ctgaaaatct tccatattag tcagtcagag gataaggcta atatcctgga
     1681 taaagacgaa cacttctacc tggtgttcga ggaatgttac ttcgagctgg caaacattgt
     1741 ccccctgtat aacaagatta ggaactacat cacacagaag ccttactctg acgagaagtt
     1801 taaactgaac ttcgaaaata gtaccctggc caacgggtgg gataagaaca aggagcctga
     1861 caacacagct atcctgttca tcaaggatga caagtactat ctgggagtga tgaataagaa
     1921 aaacaataag atcttcgatg acaaagccat taaggagaac aaaggggaag gatacaagaa
     1981 aatcgtgtat aagctgctgc ccggcgcaaa taagatgctg cctaaggtgt tcttcagcgc
     2041 caagagtatc aaattctaca acccatccga ggacatcctg cggattagaa atcactcaac
     2101 acatactaag aacgggagcc cccagaaggg atatgagaaa tttgagttca acatcgagga
     2161 ttgcaggaag tttattgact tctacaagca gagcatctcc aaacaccctg aatggaagga
     2221 ttttggcttc cggttttccg acacacagag atataactct atcgacgagt tctaccgcga
     2281 ggtggaaaat caggggtata agctgacttt tgagaacatt tctgaaagtt acatcgacag
     2341 cgtggtcaat cagggaaagc tgtacctgtt ccagatctat aacaaagatt tttcagcata
     2401 cagcaagggc agaccaaacc tgcatacact gtactggaag gccctgttcg atgagaggaa
     2461 tctgcaggac gtggtctata aactgaacgg agaggccgaa ctgttttacc ggaagcagtc
     2521 tattcctaag aaaatcactc acccagctaa ggaggccatc gctaacaaga acaaggacaa
     2581 tcctaagaaa gagagcgtgt tcgaatacga tctgattaag gacaagcggt tcaccgaaga
     2641 taagttcttt ttccattgtc caatcaccat taacttcaag tcaagcggcg ctaacaagtt
     2701 caacgacgag atcaatctgc tgctgaagga aaaagcaaac gatgtgcaca tcctgagcat
     2761 tgaccgagga gagcggcatc tggcctacta taccctggtg gatggcaaag ggaatatcat
     2821 taagcaggat acattcaaca tcattggcaa tgaccggatg aaaaccaact accacgataa
     2881 actggctgca atcgagaagg atagagactc agctaggaag gactggaaga aaatcaacaa
     2941 cattaaggag atgaaggaag gctatctgag ccaggtggtc catgagattg caaagctggt
     3001 catcgaatac aatgccattg tggtgttcga ggatctgaac ttcggcttta agagggggcg
     3061 ctttaaggtg gaaaaacagg tctatcagaa gctggagaaa atgctgatcg aaaagctgaa
     3121 ttacctggtg tttaaagata acgagttcga caagaccgga ggcgtcctga gagcctacca
     3181 gctgacagct ccctttgaaa ctttcaagaa aatgggaaaa cagacaggca tcatctacta
     3241 tgtgccagcc ggattcactt ccaagatctg ccccgtgacc ggctttgtca accagctgta
     3301 ccctaaatat gagtcagtga gcaagtccca ggaatttttc agcaagttcg ataagatctg
     3361 ttataatctg gacaaggggt acttcgagtt ttccttcgat tacaagaact tcggcgacaa
     3421 ggccgctaag gggaaatgga ccattgcctc cttcggatct cgcctgatca actttcgaaa
     3481 ttccgataaa aaccacaatt gggacactag ggaggtgtac ccaaccaagg agctggaaaa
     3541 gctgctgaaa gactactcta tcgagtatgg acatggcgaa tgcatcaagg cagccatctg
     3601 tggcgagagt gataagaaat ttttcgccaa gctgacctca gtgctgaata caatcctgca
     3661 gatgcggaac tcaaagaccg ggacagaact ggactatctg attagccccg tggctgatgt
     3721 caacggaaac ttcttcgaca gcagacaggc acccaaaaat atgcctcagg atgcagacgc
     3781 caacggggcc taccacatcg ggctgaaggg actgatgctg ctgggccgga tcaagaacaa
     3841 tcaggagggg aagaagctga acctggtcat taagaacgag gaatacttcg agtttgtcca
     3901 gaatagaaat aacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa
     3961 gggatcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc
     4021 atacccatat gatgtccccg actatgccta atcagtgaga ccgaattctc gccctatagt
     4081 gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4141 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4201 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     4261 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     4321 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4381 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4441 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4501 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4621 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4681 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4741 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4801 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4861 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4921 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4981 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5041 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5101 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5161 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5221 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5281 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5341 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5401 ggctctcgag gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5461 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5521 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5581 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5641 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5761 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5881 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5941 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6001 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6061 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6121 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6181 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6241 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6301 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6361 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6421 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6481 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6541 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6601 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6661 tgtggaattg tgagcggata acaatttcac acgagctatt taggtgacac tatagaagtg
     6721 aagctt
//
