LOCUS       pGG-C-LbCas12a-D        6510 bp    DNA     circular UNA 14-APR-2023
DEFINITION  .
ACCESSION   urn.local...t-946qqgc
VERSION     urn.local...t-946qqgc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..3835
                     /created_by="User"
                     /modified_by="User"
                     /label="hLbCpf1"
     NLS             3698..3745
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="AAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAG"
                     /annotation_group="NLS: 4,613 -> 4,660"
     Linker          3746..3751
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GGATCC"
                     /annotation_group="GS linker: 4,661 -> 4,666"
                     /label="GS linker"
     Tag             3752..3832
                     /%_Identity=100
                     /Motif="TACCCATACGATGTTCCAGATTACGCTTATCCCTACGACGTGCCTGATTA
                     TGCATACCCATATGATGTCCCCGACTATGCC"
                     /annotation_group="3X HA Tag: 4,667 -> 4,747"
                     /modified_by="mamee"
                     /label="3xHA"
     GG              complement(3836..3846)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="D"
     primer          complement(3866..3886)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        4366..4470
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    4471..5331
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5502..6090
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6454..6475
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgagca agctggagaa gtttacaaac tgctactccc tgtctaagac
       61 cctgaggttc aaggccatcc ctgtgggcaa gacccaggag aacatcgaca ataagcggct
      121 gctggtggag gacgagaaga gagccgagga ttataagggc gtgaagaagc tgctggatcg
      181 ctactatctg tcttttatca acgacgtgct gcacagcatc aagctgaaga atctgaacaa
      241 ttacatcagc ctgttccgga agaaaaccag aaccgagaag gagaataagg agctggagaa
      301 cctggagatc aatctgcgga aggagatcgc caaggccttc aagggcaacg agggctacaa
      361 gtccctgttt aagaaggata tcatcgagac aatcctgcca gagttcctgg acgataagga
      421 cgagatcgcc ctggtgaaca gcttcaatgg ctttaccaca gccttcaccg gcttctttga
      481 taacagagag aatatgtttt ccgaggaggc caagagcaca tccatcgcct tcaggtgtat
      541 caacgagaat ctgacccgct acatctctaa tatggacatc ttcgagaagg tggacgccat
      601 ctttgataag cacgaggtgc aggagatcaa ggagaagatc ctgaacagcg actatgatgt
      661 ggaggatttc tttgagggcg agttctttaa ctttgtgctg acacaggagg gcatcgacgt
      721 gtataacgcc atcatcggcg gcttcgtgac cgagagcggc gagaagatca agggcctgaa
      781 cgagtacatc aacctgtata atcagaaaac caagcagaag ctgcctaagt ttaagccact
      841 gtataagcag gtgctgagcg atcgggagtc tctgagcttc tacggcgagg gctatacatc
      901 cgatgaggag gtgctggagg tgtttagaaa caccctgaac aagaacagcg agatcttcag
      961 ctccatcaag aagctggaga agctgttcaa gaattttgac gagtactcta gcgccggcat
     1021 ctttgtgaag aacggccccg ccatcagcac aatctccaag gatatcttcg gcgagtggaa
     1081 cgtgatccgg gacaagtgga atgccgagta tgacgatatc cacctgaaga agaaggccgt
     1141 ggtgaccgag aagtacgagg acgatcggag aaagtccttc aagaagatcg gctccttttc
     1201 tctggagcag ctgcaggagt acgccgacgc cgatctgtct gtggtggaga agctgaagga
     1261 gatcatcatc cagaaggtgg atgagatcta caaggtgtat ggctcctctg agaagctgtt
     1321 cgacgccgat tttgtgctgg agaagagcct gaagaagaac gacgccgtgg tggccatcat
     1381 gaaggacctg ctggattctg tgaagagctt cgagaattac atcaaggcct tctttggcga
     1441 gggcaaggag acaaacaggg acgagtcctt ctatggcgat tttgtgctgg cctacgacat
     1501 cctgctgaag gtggaccaca tctacgatgc catccgcaat tatgtgaccc agaagcccta
     1561 ctctaaggat aagttcaagc tgtattttca gaaccctcag ttcatgggcg gctgggacaa
     1621 ggataaggag acagactatc gggccaccat cctgagatac ggctccaagt actatctggc
     1681 catcatggat aagaagtacg ccaagtgcct gcagaagatc gacaaggacg atgtgaacgg
     1741 caattacgag aagatcaact ataagctgct gcccggccct aataagatgc tgccaaaggt
     1801 gttcttttct aagaagtgga tggcctacta taaccccagc gaggacatcc agaagatcta
     1861 caagaatggc acattcaaga agggcgatat gtttaacctg aatgactgtc acaagctgat
     1921 cgacttcttt aaggatagca tctcccggta tccaaagtgg tccaatgcct acgatttcaa
     1981 cttttctgag acagagaagt ataaggacat cgccggcttt tacagagagg tggaggagca
     2041 gggctataag gtgagcttcg agtctgccag caagaaggag gtggataagc tggtggagga
     2101 gggcaagctg tatatgttcc agatctataa caaggacttt tccgataagt ctcacggcac
     2161 acccaatctg cacaccatgt acttcaagct gctgtttgac gagaacaatc acggacagat
     2221 caggctgagc ggaggagcag agctgttcat gaggcgcgcc tccctgaaga aggaggagct
     2281 ggtggtgcac ccagccaact cccctatcgc caacaagaat ccagataatc ccaagaaaac
     2341 cacaaccctg tcctacgacg tgtataagga taagaggttt tctgaggacc agtacgagct
     2401 gcacatccca atcgccatca ataagtgccc caagaacatc ttcaagatca atacagaggt
     2461 gcgcgtgctg ctgaagcacg acgataaccc ctatgtgatc ggcatcgata ggggcgagcg
     2521 caatctgctg tatatcgtgg tggtggacgg caagggcaac atcgtggagc agtattccct
     2581 gaacgagatc atcaacaact tcaacggcat caggatcaag acagattacc actctctgct
     2641 ggacaagaag gagaaggaga ggttcgaggc ccgccagaac tggacctcca tcgagaatat
     2701 caaggagctg aaggccggct atatctctca ggtggtgcac aagatctgcg agctggtgga
     2761 gaagtacgat gccgtgatcg ccctggagga cctgaactct ggctttaaga atagccgcgt
     2821 gaaggtggag aagcaggtgt atcagaagtt cgagaagatg ctgatcgata agctgaacta
     2881 catggtggac aagaagtcta atccttgtgc aacaggcggc gccctgaagg gctatcagat
     2941 caccaataag ttcgagagct ttaagtccat gtctacccag aacggcttca tcttttacat
     3001 ccctgcctgg ctgacatcca agatcgatcc atctaccggc tttgtgaacc tgctgaaaac
     3061 caagtatacc agcatcgccg attccaagaa gttcatcagc tcctttgaca ggatcatgta
     3121 cgtgcccgag gaggatctgt tcgagtttgc cctggactat aagaacttct ctcgcacaga
     3181 cgccgattac atcaagaagt ggaagctgta ctcctacggc aaccggatca gaatcttccg
     3241 gaatcctaag aagaacaacg tgttcgactg ggaggaggtg tgcctgacca gcgcctataa
     3301 ggagctgttc aacaagtacg gcatcaatta tcagcagggc gatatcagag ccctgctgtg
     3361 cgagcagtcc gacaaggcct tctactctag ctttatggcc ctgatgagcc tgatgctgca
     3421 gatgcggaac agcatcacag gccgcaccga cgtggatttt ctgatcagcc ctgtgaagaa
     3481 ctccgacggc atcttctacg atagccggaa ctatgaggcc caggagaatg ccatcctgcc
     3541 aaagaacgcc gacgccaatg gcgcctataa catcgccaga aaggtgctgt gggccatcgg
     3601 ccagttcaag aaggccgagg acgagaagct ggataaggtg aagatcgcca tctctaacaa
     3661 ggagtggctg gagtacgccc agaccagcgt gaagcacaaa aggccggcgg ccacgaaaaa
     3721 ggccggccag gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta
     3781 tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg cctaatcagt
     3841 gagaccgaat tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt
     3901 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3961 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4021 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4081 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4141 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4201 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4261 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4321 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4381 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4441 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4501 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4561 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4621 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4681 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4741 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4801 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4861 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4921 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4981 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5041 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5101 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5161 gataaatctg gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat
     5221 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5281 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5341 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5641 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5821 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5881 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5941 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6001 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6061 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6121 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6181 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6241 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6301 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6361 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6421 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc
     6481 tatttaggtg acactataga agtgaagctt
//
