LOCUS       pGG-E-tUM10_0172-F        3236 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.c2-cn405e0
VERSION     urn.local.jonasblomme.c2-cn405e0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-c4nnd55"">E</a>"
                     /label="E"
     terminator      12..561
                     /label="tUM010_0172"
     GG              complement(562..572)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29z-ciz79d7"">F</a>"
                     /label="F"
     primer          complement(592..612)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1092..1196
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1197..2057
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2228..2816
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3180..3201
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cattctctct ggtcagtgtc ctaattcagg aaccgtctga tttcgggctg
       61 tagctagaga gtgtgaaagc gagtgtaagg caaacattct gcagagatcg gccaccaaat
      121 tttagacgtg agccattgct ggctcagtct ataccatatt tctccggcac ggtgggttgt
      181 tgcataggca cgatgatgta atcttccccg gagtgtacaa cccgctggca tggtgtgcga
      241 tatgactgtt gacgctccgg caattgagag agggtgatgt cgtgaatctg tgttgtggcc
      301 aaatcaaacc ttccgaaagt taatcatcgt caactgctta catgatttgt gtcacgatta
      361 tctgtcagtt tgaacatctt gtgggcacat gatctcagaa gaaggtcgca actcaacacc
      421 gcacatgttg tacgtcacct aaatcaaagt tgtgcgtgct tgtcaacatc actctgacac
      481 aatctagctt gcagcggcta gaaaattaac taagtcagca gcgcgggttg ccaggtgcct
      541 gcaatgcaag taacatgcta tactatgaga ccgaattctc gccctatagt gagtcgtatt
      601 acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      721 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
      781 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      841 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      901 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      961 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     1021 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1081 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1141 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1201 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1261 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1321 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1381 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1441 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1501 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1561 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1621 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1681 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1741 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1801 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1861 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggctctcgag
     1921 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1981 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2041 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2101 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2161 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2221 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2281 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2341 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2401 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2461 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2521 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2581 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2641 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2701 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2761 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2821 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2881 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2941 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3001 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3061 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3181 tgagcggata acaatttcac acgagctatt taggtgacac tatagaagtg aagctt
//
