LOCUS       pGG-E-tUM056_0044-F        3086 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.c3-cn405e0
VERSION     urn.local.jonasblomme.c3-cn405e0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-c4nnd55"">E</a>"
                     /label="E"
     terminator      12..411
                     /label="tUM056_0044"
     GG              complement(412..422)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29z-ciz79d7"">F</a>"
                     /label="F"
     primer          complement(442..462)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        942..1046
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1047..1907
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2078..2666
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3030..3051
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cgctggaggc gttcttactg tactgtacac ttggggggga ttttttttat
       61 gccggcagtg caatgatgtg tgagtagccg atgtgtttca taacacatgt ccaacaatgt
      121 aattcgctga tccggctaat aaaaggtgta tggttgtcat atgcaactgt ttgtgtgaaa
      181 aaacatgccg tgcaacacgt ggcatagttc cactacaaac attccactca ttcgtaattg
      241 tatacgaatt gagtgaaatg ttatgtgggg ttgaaacccc gctcaacggg caactacgca
      301 ccctgccacc gcactaatgc cccaaaaaaa aaaccaattc gagtgtgtgc acagactggt
      361 gaacggttga acagtccaaa tataaattgc acaatcccat acatgtcctg tactatgaga
      421 ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa cgtcgtgact
      481 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      601 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      661 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
      721 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      781 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      841 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      901 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1741 aatctggagc cggtgagcgt ggctctcgag gtatcattgc agcactgggg ccagatggta
     1801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acgagctatt
     3061 taggtgacac tatagaagtg aagctt
//
