LOCUS       pGG-pUM140_0016-UM120_0017.1_Ferredoxin-YFP-NOST-PIBT3        8310 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.1h-cgtoeim
VERSION     urn.local.jonasblomme.1h-cgtoeim
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1970..2149,2705..2773,2975..3106)
                     /note
                     /label="UM120_0017.1"
     intron          2150..2704
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          2774..2974
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             3113..3832
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3837..4104
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          4109..4157
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 4170..5996
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        4170..4715
                     /label="UmpRbcS"
     CDS             join(4716..4723,5221..5587)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4724..5220
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      5588..5996
                     /label="Ulva trbcS"
     primer          complement(6001..6021)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        6082..6132
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    6133..6942
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7089..7677
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          8276..8296
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tgatccacaa
     1981 cgtccgcact gtcaagccgg catctttcgg cctcgctgcc ccgaaggccc gtttcgcggc
     2041 tgctgctcgc ccatcagtga aggctaactt caaggtgaaa ctcgagacgc ccgatggaac
     2101 aaaggaggtc gagatgaacg gtgacgagta catcgttgac caagctgagg tgcgccatct
     2161 cgtacgccaa tgccagggca gcatggagcg aagaagactt catgaactgc gactcatatg
     2221 gcatcgtggg tgccgtctag tagcatgcaa agctcgatga cagccgcgat ctcaccctgc
     2281 attctcgacg gtccgatgac tggtcaagct attcagcaag ccgagtatgc cacgtatatt
     2341 aggccacata gtatctgcca ccgcggctgt ggcattgtga tgtggtggcc ttgccaggga
     2401 agatctgccg aggcaatgcg agggttcacc ctgcgcaccc tcggcgcatt cgtggcctca
     2461 cactgcattg gtcgcctcac aacatgatgt gccgcgccct cgtagcttcg gccacgttct
     2521 agcgcatgac atttgtatca tagtccggga ttccgtttca ctactcactg caggcttgac
     2581 ggtggatctg ccggaggcct acgatgaatt cccgtcttgt gctttcacct gcgtcgatgt
     2641 gcgtcgatgt acgctcttca taaaaaatct attacgtact ggcccaccaa ttttatgctt
     2701 gcaggaggct ggcattgagc tgccgtactc ttgccgtgcc ggcacctgct cttcttgcgc
     2761 cggtctggtt gtggtacgac cccaccctcg aaggctggaa tcttcagcct gtgcggtcat
     2821 ctgttcatta gatcgcttgg caggatgctc ggactgtact ctagtgcagc tgtgcaccaa
     2881 gctgccttgt agtttctaat aaatctagat tcaccagctt gtgtcataat ggtgtgctgg
     2941 aggccaccat gataaacagt ttgccaactt gcaggagggt gaggttgatc agtccgagca
     3001 gaacttcctt gatgatgagc agatggagaa tggctatgtg ctgacctgcg ttgcttaccc
     3061 aacctcggac cttaccatca agacccacca ggaggaggag ctgtactcag ctatggtgag
     3121 caagggcgag gagctgttca cgggcgtggt gcccatcctg gtggagctgg acggcgacgt
     3181 gaacggccac aagttcagcg tgtccggcga gggcgagggc gacgccacct acggcaagct
     3241 gaccctgaag ttcatctgca cgaccggcaa gctgcccgtg ccctggccca ccctggtgac
     3301 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     3361 cttcttcaag agcgccatgc cggagggcta cgtgcaggag cgcaccatct tcttcaagga
     3421 cgacggcaac tacaagaccc gcgcggaggt gaagttcgag ggcgacacgc tggtgaaccg
     3481 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctgggcc acaagctgga
     3541 gtacaactac aacagccaca acgtgtacat catggccgac aagcagaaga acggcatcaa
     3601 ggtgaacttc aagatccgcc acaacatcga ggacggctcg gtgcagctgg cggaccacta
     3661 ccagcagaac acccccatcg gcgacggccc ggtgctgctg cccgacaacc actacctgtc
     3721 ctaccagagc gccctgtcca aggaccccaa cgagaagcgc gaccacatgg tgctgctgga
     3781 gttcgtgacc gccgccggca tcaccctggg catggacgag ctgtacaagt gactgcctag
     3841 agtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     3901 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     3961 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     4021 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     4081 gtgtcatcta tgttactaga tcgaactata agctaggtag taactagtgc gactgtctag
     4141 aatcgcgtat gcgagtgtat gagctcgatc gttgttcgta cacaaggatg tcgcttgtgg
     4201 ctttttcgct cggctttcca ggagggcagt ccagatacca tgcggccgcc gtgtccatca
     4261 ctcgcgctgc agctgatgtg ggcagatact gcactaatct gccgcacgac gcgtcggcct
     4321 gagtcggcgg acaccggagg cttgctggag tactggcaca agtgtgcgtg tgattgccag
     4381 aaacagcaac ccactcacta gatataccgc tccaataatg ggttcatgtg caagctcact
     4441 caataacgaa acgcttcact attgtgcacg tgccggggtt gcttgggtat ggcgtgccat
     4501 acgggttctg cacatgtcac ccacgtgggt gtggcacgag atatcgaggt ggccatgtaa
     4561 aaggcatttg ctagagggca gctcgtccat gccgctcgag cacaccgagt gcgcaccata
     4621 aatgcagcac atgcctgtgc agcctatctc ctgtctctgt tacatcccac cacctcctca
     4681 catatcccct tccaccctta aaccgcacag cagacatggc caagtgcgca tcgcgtgtca
     4741 atgctggcgc tgctgacgtc cgctgcctaa actgcatgaa agctcctgca gtcgcaagta
     4801 gtccttttaa cttgttgtat cggaacaaat ggttgtagtc tgtgccgtgt gacgatcttg
     4861 agccgcctgg tcggcaggtc caatgtcggc ctggaccctg cacaccctgc ccaccctgcc
     4921 gctgctcacc cgttcacgac cccacaggtg ctcgcagcag taattgtcga tagatagcaa
     4981 ctgtgttctg actattcttt gaccttccgc atttgggact gcggcggcac acctactatc
     5041 cgcaatgcac accctggtgc tggtactgcc tgcctttccg cgcgagccca caccatgcag
     5101 gcgcatagta atggactgtg attgatttag gcaactgtat gaaacctctt catcgtgcct
     5161 tggttgcttg cgataccctc acgtgagttc atatgaccaa cgctggcacg acccctgcag
     5221 gatcaccagc gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     5281 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     5341 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     5401 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     5461 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     5521 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     5581 ggactaagca gtcaggcaga gtcgttgttg ggtgtatggt tgcacaaagc tagacctcga
     5641 gctggtcggt tagaaagaga tgcttgtgcg actggatgta acaggcacaa tccgaggaaa
     5701 atgcaacaga aacgttatga tgtaacatct gagcacagtc aataaaattg catgttgtat
     5761 gccgcacaca aaagtgttgt taaacatgcg atgtggttgt caaatcatta ttcgcacaac
     5821 catggaccga acctctggca cgctgcatac gctgtattca ttcacatcat gttttctact
     5881 tgtcacatgc tctgcaccaa gtaactccga ttagttaggt tcgcacatgt catcggagtg
     5941 gaccacttcg aatcatttgg ttcgaagata aaccggatcc cctatagtga gtcgtattac
     6001 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     6061 gcacaagata aaataatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     6121 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     6181 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     6241 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     6301 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     6361 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     6421 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     6481 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     6541 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     6601 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     6661 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     6721 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     6781 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     6841 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     6901 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     6961 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     7021 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7201 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7861 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7921 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     7981 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     8041 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     8101 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     8161 aacagataaa acgaaaggcc cagacttccg actgagcctt tcgttttatt tgatgcctgg
     8221 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     8281 acgacggcca gtcttaagct cgggcccacc
//
