LOCUS       pGG-pUM140_0016-UM119_0052.1_Vacuole-YFP-NOST-PIBT3        8378 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.1g-cgtoeil
VERSION     urn.local.jonasblomme.1g-cgtoeil
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1970..1984,2068..2154,2240..2340,2434..2587,
                     2656..2864,3054..3174)
                     /note
                     /label="UM119_0052.1"
     intron          1985..2067
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          2155..2239
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          2341..2433
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          2588..2655
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          2865..3053
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             3181..3900
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3905..4172
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          4177..4225
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 4238..6064
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        4238..4783
                     /label="UmpRbcS"
     CDS             join(4784..4791,5289..5655)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4792..5288
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      5656..6064
                     /label="Ulva trbcS"
     primer          complement(6069..6089)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        6150..6200
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    6201..7010
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7157..7745
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          8344..8364
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tgaacgagag
     1981 tgaggtgcgc tttgacatgt cgtaccggcc gagtgaagcg tctggtgcat aacgcgatgg
     2041 aggccttttg aaccaccgat cctgtaggtc aacagacaga ttgaacagat ggtgatgttc
     2101 atcaagcagg aagctgagga aaaagccaac gaaatccgca tgtctgctga ggaggcgcgt
     2161 cacccgcttg tccagaatgc cttttgttct gatgtttcaa caaattaact cagcggccga
     2221 ttaaaatgca tgcatgcagg aatttaatct ggaaaagctc aacctgctcg aaactgaaaa
     2281 gcagcgcatc cgcaaagaat acgagcgcaa ggaggcgcag gtggaggcca acaaaaagat
     2341 gtgcgcagtc aaatttaagt gtgcgtgtga gccggaaaaa tatggcattg cgtacctcat
     2401 atctcagcgt tgacccgacc gactgtgccg cagtgaggcc tcgaagcagc ataatgaaca
     2461 acggctgaaa gtgcttggtg caaagcagcg cttgatcaaa gacgtgttac aggctgcaaa
     2521 ggatgagttg aagaagaaag ctggtggtag tggttacgct gatctcgtga gcgacctttt
     2581 ggtgcaggta tgcttcgctg tttgcgtgcg ctgtgacttg taacatcgct ctcaaatgca
     2641 cgtgactata tttaggcgat gtacaaactt caagagccaa gccagatcgt tcagtgccgg
     2701 gaggcagatc tgaaggtcat agagtcatgt gtgccaaagg cacaggagaa gtataaaagt
     2761 gtatatggca gtgatgcacc gcagctcacc attgatcgca agcactttct tcccgcagct
     2821 gcaacatctc aggaacaaga ggatgacccg gatgcgccta cttggtgcgg cagctgaatt
     2881 tgtgatcagc tcgggtgata agttgtttgt gatagctctc tttgcacagt ttgtgttttt
     2941 ctgctgcatg tcgctctctc atttaccatt ttacctgagg gacactttct gactacctgc
     3001 ggacttggtc tactaacaat tacattctgc taatttgagt tgagtttgtg cagcctggga
     3061 ggtgttgtgg tgacctctat tagtggccgc attgttgtcg ataacacact ggacgccagg
     3121 ctcaaaatcg tcgaggacaa gcagcttcca gcgatcagag ctcaattgtt cgagtcagct
     3181 atggtgagca agggcgagga gctgttcacg ggcgtggtgc ccatcctggt ggagctggac
     3241 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga cgccacctac
     3301 ggcaagctga ccctgaagtt catctgcacg accggcaagc tgcccgtgcc ctggcccacc
     3361 ctggtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     3421 cagcacgact tcttcaagag cgccatgccg gagggctacg tgcaggagcg caccatcttc
     3481 ttcaaggacg acggcaacta caagacccgc gcggaggtga agttcgaggg cgacacgctg
     3541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac
     3601 aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa gcagaagaac
     3661 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctcggt gcagctggcg
     3721 gaccactacc agcagaacac ccccatcggc gacggcccgg tgctgctgcc cgacaaccac
     3781 tacctgtcct accagagcgc cctgtccaag gaccccaacg agaagcgcga ccacatggtg
     3841 ctgctggagt tcgtgaccgc cgccggcatc accctgggca tggacgagct gtacaagtga
     3901 ctgcctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3961 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     4021 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     4081 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     4141 cgcgcgcggt gtcatctatg ttactagatc gaactataag ctaggtagta actagtgcga
     4201 ctgtctagaa tcgcgtatgc gagtgtatga gctcgatcgt tgttcgtaca caaggatgtc
     4261 gcttgtggct ttttcgctcg gctttccagg agggcagtcc agataccatg cggccgccgt
     4321 gtccatcact cgcgctgcag ctgatgtggg cagatactgc actaatctgc cgcacgacgc
     4381 gtcggcctga gtcggcggac accggaggct tgctggagta ctggcacaag tgtgcgtgtg
     4441 attgccagaa acagcaaccc actcactaga tataccgctc caataatggg ttcatgtgca
     4501 agctcactca ataacgaaac gcttcactat tgtgcacgtg ccggggttgc ttgggtatgg
     4561 cgtgccatac gggttctgca catgtcaccc acgtgggtgt ggcacgagat atcgaggtgg
     4621 ccatgtaaaa ggcatttgct agagggcagc tcgtccatgc cgctcgagca caccgagtgc
     4681 gcaccataaa tgcagcacat gcctgtgcag cctatctcct gtctctgtta catcccacca
     4741 cctcctcaca tatccccttc cacccttaaa ccgcacagca gacatggcca agtgcgcatc
     4801 gcgtgtcaat gctggcgctg ctgacgtccg ctgcctaaac tgcatgaaag ctcctgcagt
     4861 cgcaagtagt ccttttaact tgttgtatcg gaacaaatgg ttgtagtctg tgccgtgtga
     4921 cgatcttgag ccgcctggtc ggcaggtcca atgtcggcct ggaccctgca caccctgccc
     4981 accctgccgc tgctcacccg ttcacgaccc cacaggtgct cgcagcagta attgtcgata
     5041 gatagcaact gtgttctgac tattctttga ccttccgcat ttgggactgc ggcggcacac
     5101 ctactatccg caatgcacac cctggtgctg gtactgcctg cctttccgcg cgagcccaca
     5161 ccatgcaggc gcatagtaat ggactgtgat tgatttaggc aactgtatga aacctcttca
     5221 tcgtgccttg gttgcttgcg ataccctcac gtgagttcat atgaccaacg ctggcacgac
     5281 ccctgcagga tcaccagcgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     5341 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     5401 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     5461 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     5521 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     5581 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     5641 gaggagcagg actaagcagt caggcagagt cgttgttggg tgtatggttg cacaaagcta
     5701 gacctcgagc tggtcggtta gaaagagatg cttgtgcgac tggatgtaac aggcacaatc
     5761 cgaggaaaat gcaacagaaa cgttatgatg taacatctga gcacagtcaa taaaattgca
     5821 tgttgtatgc cgcacacaaa agtgttgtta aacatgcgat gtggttgtca aatcattatt
     5881 cgcacaacca tggaccgaac ctctggcacg ctgcatacgc tgtattcatt cacatcatgt
     5941 tttctacttg tcacatgctc tgcaccaagt aactccgatt agttaggttc gcacatgtca
     6001 tcggagtgga ccacttcgaa tcatttggtt cgaagataaa ccggatcccc tatagtgagt
     6061 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     6121 gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa
     6181 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     6241 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     6301 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     6361 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     6421 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     6481 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     6541 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     6601 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     6661 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     6721 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     6781 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     6841 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     6901 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     6961 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     7021 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     7081 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     7141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     8041 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     8101 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     8161 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     8221 cgacaaacaa cagataaaac gaaaggccca gacttccgac tgagcctttc gttttatttg
     8281 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     8341 gttgtaaaac gacggccagt cttaagctcg ggcccacc
//
