LOCUS pGG-pUM140_0016-UM021_0090.1_TUA2-YFP-NOST-PIBT3 9130 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.1d-cgtoeik
VERSION urn.local.jonasblomme.1d-cgtoeik
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
promoter 2..1935
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(1970..2074,2576..3716,3817..3926)
/note
/label="UM021_0090.1"
intron 2075..2575
/created_by="jonasblomme"
/label="Intron"
intron 3717..3816
/created_by="jonasblomme"
/label="Intron"
CDS 3933..4652
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
/modified_by="jonasblomme"
/label="YFP"
terminator 4657..4924
/vntifkey=43
/modified_by="mamee"
/label="tNOS"
Linker 4929..4977
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
Plant_Selection 4990..6816
/created_by="mamee"
/modified_by="mamee"
/label="PIBT3"
promoter 4990..5535
/label="UmpRbcS"
CDS join(5536..5543,6041..6407)
/modified_by="jonasblomme"
/label="ble"
intron 5544..6040
/gene="ble"
/note="Ulva mutabilis RbcS intron 1; enhancer"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RbcsI"
/Transferred_Similarity="99.40%"
/Primary_Match="99.40%; Adjusted-Interval-0:0/0; RbcsI"
/modified_by="jonasblomme"
/label="RbcsI"
terminator 6408..6816
/modified_by="mamee"
/label="Ulva tRbcS"
primer complement(6821..6841)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 6902..6952
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 6953..7762
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 7909..8497
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 9096..9116
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tgcgtgaggt
1981 tatctccatt cacatcggcc aggccggcat ccaagtcgga aatgcctgct gggagcttta
2041 ttgtcttgag cacggaattc agcccgatgg gcaggtacgt cttgttcgag tttgcctcgt
2101 tcgttcgttc aggactttca ggcagttgca gctttggacc ccacaatcgc catttacaat
2161 cctcatatgt agtggattgt tattgctctg ctttgactat gatcacattt tcaagttcat
2221 cgaagctatt ttgtctctga cgctcgagtt gcccgacact ttgtgaacgg gtcgatactt
2281 cggatgttaa aatgtggctc aaataccatc aaatatgacg ttatgcatct attacgccgt
2341 ttaaaatatg catagtggtt gtcgcggcag ctattgcttg tttcagacct aaatttaaaa
2401 tagcggttgc accagacgct atttgaaggc gtcccgcaag ccatgtttga cttgaaagcc
2461 atccgtattg tgaattcgat gatttatctc gatcagcctt gaatgaagtg tccgaaatgc
2521 catcgcgagc ccattgtggc aacacgtgcg ctctcacgac gtactgctct tgcagatgcc
2581 ttctgacaag accatcggcg gaggtgacga cgctttcaac accttcttct ccgagactgg
2641 tgctggcaaa catgtccctc gtgctgtttt cctcgacttg gagcctaccg tcatcgacga
2701 agtccgcacc ggcacctacc gccagctgtt ccaccccgag cagctcatct caggcaagga
2761 ggatgccgct aacaacttcg ctcgtggcca ctacaccatc ggcaaggaga ttgttgacct
2821 ttgccttgac cgcattcgca agcttgcgga caactgcaca ggtctgcagg gcttcctggt
2881 cttcaacgct gtcggtggtg gtaccggctc cggcctgggc tctctcctcc tggagcgtct
2941 gtctgttgac tacggcaaga agtccaagct cggcttcacc gtctacccgt cccctcaagt
3001 ctcgactgcc gttgtagagc catacaactc tgtcttgtcc acccactctc tgctggagca
3061 cactgatgtt gctgtcatgc ttgacaacga ggctgtgtat gacatctgcc gccgctctct
3121 tgacatcgag cgccctacat acaccaacct gaacaggctc atcgctcagg tcatttcatc
3181 cctgactgct tctctgcgat ttgatggtgc cctcaacgtt gatgtgaccg agttccagac
3241 caaccttgtg ccctaccctc gcatccattt catgctttcc tcctacgcgc ctgtcatctc
3301 tgccgagaag gcataccatg agcagctgtc tgtggctgag atcaccaact ctgccttcga
3361 gcctgcctcc atgatggcta agtgtgaccc tcgtcatggc aagtacatgg cctgctgcct
3421 gatgtaccgt ggtgatgttg tccccaagga tgtcaatgcc gctgtcgcta ccatcaagac
3481 caagcgtacc atccagtttg ttgactggtg ccccaccggc ttcaagtgcg gcatcaacta
3541 ccagccaccc acggttgtcc caggaggtga ccttgccaag gtacaacgtg ccgtgtgcat
3601 gatctccaac tccaccgcca tcgctgaggt gttctcccgc ctggaccaca agttcgacct
3661 catgtacgcc aagcgtgcct tcgtgcactg gtatgtcggc gagggcatgg aggaaggtaa
3721 gtctttacgt ccataacttg ttcgtccttg cgctgccatc gttcccatta caggctagat
3781 ccattgtttc cttgttctga agattaaatt gtgtaggtga attctctgag gcccgtgagg
3841 atcttgctgc tctggagaag gactacgagg aggttggtgc tgagagtgct gagggtgagg
3901 gtgaagagga aggtgaggag ggctactcag ctatggtgag caagggcgag gagctgttca
3961 cgggcgtggt gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg
4021 tgtccggcga gggcgagggc gacgccacct acggcaagct gaccctgaag ttcatctgca
4081 cgaccggcaa gctgcccgtg ccctggccca ccctggtgac caccttcggc tacggcctgc
4141 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag agcgccatgc
4201 cggagggcta cgtgcaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
4261 gcgcggaggt gaagttcgag ggcgacacgc tggtgaaccg catcgagctg aagggcatcg
4321 acttcaagga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca
4381 acgtgtacat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
4441 acaacatcga ggacggctcg gtgcagctgg cggaccacta ccagcagaac acccccatcg
4501 gcgacggccc ggtgctgctg cccgacaacc actacctgtc ctaccagagc gccctgtcca
4561 aggaccccaa cgagaagcgc gaccacatgg tgctgctgga gttcgtgacc gccgccggca
4621 tcaccctggg catggacgag ctgtacaagt gactgcctag agtcaagcag atcgttcaaa
4681 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
4741 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
4801 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
4861 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
4921 tcgaactata agctaggtag taactagtgc gactgtctag aatcgcgtat gcgagtgtat
4981 gagctcgatc gttgttcgta cacaaggatg tcgcttgtgg ctttttcgct cggctttcca
5041 ggagggcagt ccagatacca tgcggccgcc gtgtccatca ctcgcgctgc agctgatgtg
5101 ggcagatact gcactaatct gccgcacgac gcgtcggcct gagtcggcgg acaccggagg
5161 cttgctggag tactggcaca agtgtgcgtg tgattgccag aaacagcaac ccactcacta
5221 gatataccgc tccaataatg ggttcatgtg caagctcact caataacgaa acgcttcact
5281 attgtgcacg tgccggggtt gcttgggtat ggcgtgccat acgggttctg cacatgtcac
5341 ccacgtgggt gtggcacgag atatcgaggt ggccatgtaa aaggcatttg ctagagggca
5401 gctcgtccat gccgctcgag cacaccgagt gcgcaccata aatgcagcac atgcctgtgc
5461 agcctatctc ctgtctctgt tacatcccac cacctcctca catatcccct tccaccctta
5521 aaccgcacag cagacatggc caagtgcgca tcgcgtgtca atgctggcgc tgctgacgtc
5581 cgctgcctaa actgcatgaa agctcctgca gtcgcaagta gtccttttaa cttgttgtat
5641 cggaacaaat ggttgtagtc tgtgccgtgt gacgatcttg agccgcctgg tcggcaggtc
5701 caatgtcggc ctggaccctg cacaccctgc ccaccctgcc gctgctcacc cgttcacgac
5761 cccacaggtg ctcgcagcag taattgtcga tagatagcaa ctgtgttctg actattcttt
5821 gaccttccgc atttgggact gcggcggcac acctactatc cgcaatgcac accctggtgc
5881 tggtactgcc tgcctttccg cgcgagccca caccatgcag gcgcatagta atggactgtg
5941 attgatttag gcaactgtat gaaacctctt catcgtgcct tggttgcttg cgataccctc
6001 acgtgagttc atatgaccaa cgctggcacg acccctgcag gatcaccagc gccgttccgg
6061 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
6121 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
6181 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
6241 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
6301 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
6361 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactaagca gtcaggcaga
6421 gtcgttgttg ggtgtatggt tgcacaaagc tagacctcga gctggtcggt tagaaagaga
6481 tgcttgtgcg actggatgta acaggcacaa tccgaggaaa atgcaacaga aacgttatga
6541 tgtaacatct gagcacagtc aataaaattg catgttgtat gccgcacaca aaagtgttgt
6601 taaacatgcg atgtggttgt caaatcatta ttcgcacaac catggaccga acctctggca
6661 cgctgcatac gctgtattca ttcacatcat gttttctact tgtcacatgc tctgcaccaa
6721 gtaactccga ttagttaggt tcgcacatgt catcggagtg gaccacttcg aatcatttgg
6781 ttcgaagata aaccggatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
6841 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaataatatc
6901 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
6961 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
7021 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
7081 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
7141 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
7201 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
7261 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
7321 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
7381 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
7441 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
7501 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
7561 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
7621 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
7681 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
7741 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
7801 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
7861 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
7921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
7981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
8041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
8101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
8161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
8221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
8281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
8341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
8401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
8461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
8521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
8581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
8641 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
8701 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
8761 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
8821 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
8881 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
8941 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
9001 cagacttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
9061 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
9121 cgggcccacc
//