LOCUS pGG-pUM140_0016-UM021_0090.1_TUA2-YFP-NOST-PIBT3 9130 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.1d-cgtoeik VERSION urn.local.jonasblomme.1d-cgtoeik KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 2..1935 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(1970..2074,2576..3716,3817..3926) /note /label="UM021_0090.1" intron 2075..2575 /created_by="jonasblomme" /label="Intron" intron 3717..3816 /created_by="jonasblomme" /label="Intron" CDS 3933..4652 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /modified_by="jonasblomme" /label="YFP" terminator 4657..4924 /vntifkey=43 /modified_by="mamee" /label="tNOS" Linker 4929..4977 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" Plant_Selection 4990..6816 /created_by="mamee" /modified_by="mamee" /label="PIBT3" promoter 4990..5535 /label="UmpRbcS" CDS join(5536..5543,6041..6407) /modified_by="jonasblomme" /label="ble" intron 5544..6040 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RbcsI" /Transferred_Similarity="99.40%" /Primary_Match="99.40%; Adjusted-Interval-0:0/0; RbcsI" /modified_by="jonasblomme" /label="RbcsI" terminator 6408..6816 /modified_by="mamee" /label="Ulva tRbcS" primer complement(6821..6841) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 6902..6952 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 6953..7762 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 7909..8497 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 9096..9116 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca 61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg 121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg 181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag 241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca 301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt 361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt 421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct 481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta 541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt 601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt 661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag 721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat 781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg 841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg 901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg 961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag 1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga 1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg 1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg 1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg 1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct 1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag 1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc 1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt 1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat 1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc 1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg 1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa 1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt 1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc 1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc 1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tgcgtgaggt 1981 tatctccatt cacatcggcc aggccggcat ccaagtcgga aatgcctgct gggagcttta 2041 ttgtcttgag cacggaattc agcccgatgg gcaggtacgt cttgttcgag tttgcctcgt 2101 tcgttcgttc aggactttca ggcagttgca gctttggacc ccacaatcgc catttacaat 2161 cctcatatgt agtggattgt tattgctctg ctttgactat gatcacattt tcaagttcat 2221 cgaagctatt ttgtctctga cgctcgagtt gcccgacact ttgtgaacgg gtcgatactt 2281 cggatgttaa aatgtggctc aaataccatc aaatatgacg ttatgcatct attacgccgt 2341 ttaaaatatg catagtggtt gtcgcggcag ctattgcttg tttcagacct aaatttaaaa 2401 tagcggttgc accagacgct atttgaaggc gtcccgcaag ccatgtttga cttgaaagcc 2461 atccgtattg tgaattcgat gatttatctc gatcagcctt gaatgaagtg tccgaaatgc 2521 catcgcgagc ccattgtggc aacacgtgcg ctctcacgac gtactgctct tgcagatgcc 2581 ttctgacaag accatcggcg gaggtgacga cgctttcaac accttcttct ccgagactgg 2641 tgctggcaaa catgtccctc gtgctgtttt cctcgacttg gagcctaccg tcatcgacga 2701 agtccgcacc ggcacctacc gccagctgtt ccaccccgag cagctcatct caggcaagga 2761 ggatgccgct aacaacttcg ctcgtggcca ctacaccatc ggcaaggaga ttgttgacct 2821 ttgccttgac cgcattcgca agcttgcgga caactgcaca ggtctgcagg gcttcctggt 2881 cttcaacgct gtcggtggtg gtaccggctc cggcctgggc tctctcctcc tggagcgtct 2941 gtctgttgac tacggcaaga agtccaagct cggcttcacc gtctacccgt cccctcaagt 3001 ctcgactgcc gttgtagagc catacaactc tgtcttgtcc acccactctc tgctggagca 3061 cactgatgtt gctgtcatgc ttgacaacga ggctgtgtat gacatctgcc gccgctctct 3121 tgacatcgag cgccctacat acaccaacct gaacaggctc atcgctcagg tcatttcatc 3181 cctgactgct tctctgcgat ttgatggtgc cctcaacgtt gatgtgaccg agttccagac 3241 caaccttgtg ccctaccctc gcatccattt catgctttcc tcctacgcgc ctgtcatctc 3301 tgccgagaag gcataccatg agcagctgtc tgtggctgag atcaccaact ctgccttcga 3361 gcctgcctcc atgatggcta agtgtgaccc tcgtcatggc aagtacatgg cctgctgcct 3421 gatgtaccgt ggtgatgttg tccccaagga tgtcaatgcc gctgtcgcta ccatcaagac 3481 caagcgtacc atccagtttg ttgactggtg ccccaccggc ttcaagtgcg gcatcaacta 3541 ccagccaccc acggttgtcc caggaggtga ccttgccaag gtacaacgtg ccgtgtgcat 3601 gatctccaac tccaccgcca tcgctgaggt gttctcccgc ctggaccaca agttcgacct 3661 catgtacgcc aagcgtgcct tcgtgcactg gtatgtcggc gagggcatgg aggaaggtaa 3721 gtctttacgt ccataacttg ttcgtccttg cgctgccatc gttcccatta caggctagat 3781 ccattgtttc cttgttctga agattaaatt gtgtaggtga attctctgag gcccgtgagg 3841 atcttgctgc tctggagaag gactacgagg aggttggtgc tgagagtgct gagggtgagg 3901 gtgaagagga aggtgaggag ggctactcag ctatggtgag caagggcgag gagctgttca 3961 cgggcgtggt gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg 4021 tgtccggcga gggcgagggc gacgccacct acggcaagct gaccctgaag ttcatctgca 4081 cgaccggcaa gctgcccgtg ccctggccca ccctggtgac caccttcggc tacggcctgc 4141 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag agcgccatgc 4201 cggagggcta cgtgcaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 4261 gcgcggaggt gaagttcgag ggcgacacgc tggtgaaccg catcgagctg aagggcatcg 4321 acttcaagga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca 4381 acgtgtacat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 4441 acaacatcga ggacggctcg gtgcagctgg cggaccacta ccagcagaac acccccatcg 4501 gcgacggccc ggtgctgctg cccgacaacc actacctgtc ctaccagagc gccctgtcca 4561 aggaccccaa cgagaagcgc gaccacatgg tgctgctgga gttcgtgacc gccgccggca 4621 tcaccctggg catggacgag ctgtacaagt gactgcctag agtcaagcag atcgttcaaa 4681 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat 4741 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt 4801 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa 4861 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga 4921 tcgaactata agctaggtag taactagtgc gactgtctag aatcgcgtat gcgagtgtat 4981 gagctcgatc gttgttcgta cacaaggatg tcgcttgtgg ctttttcgct cggctttcca 5041 ggagggcagt ccagatacca tgcggccgcc gtgtccatca ctcgcgctgc agctgatgtg 5101 ggcagatact gcactaatct gccgcacgac gcgtcggcct gagtcggcgg acaccggagg 5161 cttgctggag tactggcaca agtgtgcgtg tgattgccag aaacagcaac ccactcacta 5221 gatataccgc tccaataatg ggttcatgtg caagctcact caataacgaa acgcttcact 5281 attgtgcacg tgccggggtt gcttgggtat ggcgtgccat acgggttctg cacatgtcac 5341 ccacgtgggt gtggcacgag atatcgaggt ggccatgtaa aaggcatttg ctagagggca 5401 gctcgtccat gccgctcgag cacaccgagt gcgcaccata aatgcagcac atgcctgtgc 5461 agcctatctc ctgtctctgt tacatcccac cacctcctca catatcccct tccaccctta 5521 aaccgcacag cagacatggc caagtgcgca tcgcgtgtca atgctggcgc tgctgacgtc 5581 cgctgcctaa actgcatgaa agctcctgca gtcgcaagta gtccttttaa cttgttgtat 5641 cggaacaaat ggttgtagtc tgtgccgtgt gacgatcttg agccgcctgg tcggcaggtc 5701 caatgtcggc ctggaccctg cacaccctgc ccaccctgcc gctgctcacc cgttcacgac 5761 cccacaggtg ctcgcagcag taattgtcga tagatagcaa ctgtgttctg actattcttt 5821 gaccttccgc atttgggact gcggcggcac acctactatc cgcaatgcac accctggtgc 5881 tggtactgcc tgcctttccg cgcgagccca caccatgcag gcgcatagta atggactgtg 5941 attgatttag gcaactgtat gaaacctctt catcgtgcct tggttgcttg cgataccctc 6001 acgtgagttc atatgaccaa cgctggcacg acccctgcag gatcaccagc gccgttccgg 6061 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct 6121 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 6181 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 6241 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 6301 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 6361 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactaagca gtcaggcaga 6421 gtcgttgttg ggtgtatggt tgcacaaagc tagacctcga gctggtcggt tagaaagaga 6481 tgcttgtgcg actggatgta acaggcacaa tccgaggaaa atgcaacaga aacgttatga 6541 tgtaacatct gagcacagtc aataaaattg catgttgtat gccgcacaca aaagtgttgt 6601 taaacatgcg atgtggttgt caaatcatta ttcgcacaac catggaccga acctctggca 6661 cgctgcatac gctgtattca ttcacatcat gttttctact tgtcacatgc tctgcaccaa 6721 gtaactccga ttagttaggt tcgcacatgt catcggagtg gaccacttcg aatcatttgg 6781 ttcgaagata aaccggatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 6841 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaataatatc 6901 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca 6961 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg 7021 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg 7081 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 7141 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 7201 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac 7261 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 7321 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 7381 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 7441 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 7501 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 7561 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 7621 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 7681 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 7741 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat 7801 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc 7861 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 7921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 7981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 8041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 8101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 8161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 8221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 8281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 8341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 8401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 8461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 8521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 8581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 8641 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 8701 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 8761 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga 8821 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat 8881 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc 8941 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc 9001 cagacttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt 9061 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct 9121 cgggcccacc //