LOCUS       pGG-pUM140_0016-UM017_0086.1_Cytc6-YFP-NOST-PIBT3        8327 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.1c-cgtoeij
VERSION     urn.local.jonasblomme.1c-cgtoeij
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1970..2131,3019..3123)
                     /note
                     /label="UM017_0086.1"
     intron          2132..3018
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             3130..3849
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3854..4121
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          4126..4174
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 4187..6013
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        4187..4732
                     /label="UmpRbcS"
     CDS             join(4733..4740,5238..5604)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4741..5237
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      5605..6013
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(6018..6038)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        6099..6149
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    6150..6959
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7106..7694
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          8293..8313
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tggctgctga
     1981 ccttgcgctc ggcgaggagg tcttcagtgc aaactgcgct gtctgccaca gcggaggcaa
     2041 gaacgtgctc atccccacca aaaccctcga gaaggccgct attgagaagt acctcgaggg
     2101 aggcttcaac ctttcctcta tcctgtacca ggtgcgcgtt cggcgaatcg atgttgaccg
     2161 agttcaagtg catacagaca atttcccaag cgcccggaac gagcatcatg cacgtgacgg
     2221 cgccaatgct aggcaacgga cgcttcgaag tccctgaata gacagatctt tgtagaagcg
     2281 tggttgagca gtacttctgg gttggttatt gataacaaac gcagaggtag gggtgcttct
     2341 gtacgaacaa tctccggtac tagtactgat tcttgggtac ccgctggatc tgcagtaccg
     2401 gcgcgccggc agtacccgcg atcgcgagct gcacccacgt ggccttgggg actcgaagcg
     2461 agcgctgagt cacgccggta cctctgcgcg cagctgcgta cttgctatcg aaatcgagtt
     2521 tagactgacc ggctgtgata tcgatagcat gggcatgagg cagcatccaa tactgcctag
     2581 gccaatcatg catgctcatg atcaaaccac atgcaccttg caccttattg agttacaaac
     2641 cactgtccac gacataactc gtcactggtc accggtcatc gcagcctgta ttagctgtgc
     2701 gaccagcagc acccagtgta cctgctactt ctttgacagt tcgacgcgca ctgaacactt
     2761 ggacttcgtt gtgcaacatg ccacgaaggc tgtcaaagta tgcgtggctg cagctagtgg
     2821 cactggcatg agttctgtca ggcatcatca tgatggtatg tcagaagcca tacgtctgcc
     2881 atcaccttga tgagcaaact ggctcacagc agcgcttgac agtgcagact agtgatggct
     2941 tggctgaata gtgccatgtc catagcctga tctactgaag gttacaccaa tgctgatatt
     3001 ggagcggtgg gcatgcaggt gcagaatggc aagggccaga tgcccgcctg ggaaggtgtt
     3061 cttgaggacg aggagatcga tgctgtctcc aactacgtct acgcgaccgc ccaggctgga
     3121 tggtcagcta tggtgagcaa gggcgaggag ctgttcacgg gcgtggtgcc catcctggtg
     3181 gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgac
     3241 gccacctacg gcaagctgac cctgaagttc atctgcacga ccggcaagct gcccgtgccc
     3301 tggcccaccc tggtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac
     3361 cacatgaagc agcacgactt cttcaagagc gccatgccgg agggctacgt gcaggagcgc
     3421 accatcttct tcaaggacga cggcaactac aagacccgcg cggaggtgaa gttcgagggc
     3481 gacacgctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3541 ctgggccaca agctggagta caactacaac agccacaacg tgtacatcat ggccgacaag
     3601 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggctcggtg
     3661 cagctggcgg accactacca gcagaacacc cccatcggcg acggcccggt gctgctgccc
     3721 gacaaccact acctgtccta ccagagcgcc ctgtccaagg accccaacga gaagcgcgac
     3781 cacatggtgc tgctggagtt cgtgaccgcc gccggcatca ccctgggcat ggacgagctg
     3841 tacaagtgac tgcctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa
     3901 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     3961 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     4021 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     4081 ataaattatc gcgcgcggtg tcatctatgt tactagatcg aactataagc taggtagtaa
     4141 ctagtgcgac tgtctagaat cgcgtatgcg agtgtatgag ctcgatcgtt gttcgtacac
     4201 aaggatgtcg cttgtggctt tttcgctcgg ctttccagga gggcagtcca gataccatgc
     4261 ggccgccgtg tccatcactc gcgctgcagc tgatgtgggc agatactgca ctaatctgcc
     4321 gcacgacgcg tcggcctgag tcggcggaca ccggaggctt gctggagtac tggcacaagt
     4381 gtgcgtgtga ttgccagaaa cagcaaccca ctcactagat ataccgctcc aataatgggt
     4441 tcatgtgcaa gctcactcaa taacgaaacg cttcactatt gtgcacgtgc cggggttgct
     4501 tgggtatggc gtgccatacg ggttctgcac atgtcaccca cgtgggtgtg gcacgagata
     4561 tcgaggtggc catgtaaaag gcatttgcta gagggcagct cgtccatgcc gctcgagcac
     4621 accgagtgcg caccataaat gcagcacatg cctgtgcagc ctatctcctg tctctgttac
     4681 atcccaccac ctcctcacat atccccttcc acccttaaac cgcacagcag acatggccaa
     4741 gtgcgcatcg cgtgtcaatg ctggcgctgc tgacgtccgc tgcctaaact gcatgaaagc
     4801 tcctgcagtc gcaagtagtc cttttaactt gttgtatcgg aacaaatggt tgtagtctgt
     4861 gccgtgtgac gatcttgagc cgcctggtcg gcaggtccaa tgtcggcctg gaccctgcac
     4921 accctgccca ccctgccgct gctcacccgt tcacgacccc acaggtgctc gcagcagtaa
     4981 ttgtcgatag atagcaactg tgttctgact attctttgac cttccgcatt tgggactgcg
     5041 gcggcacacc tactatccgc aatgcacacc ctggtgctgg tactgcctgc ctttccgcgc
     5101 gagcccacac catgcaggcg catagtaatg gactgtgatt gatttaggca actgtatgaa
     5161 acctcttcat cgtgccttgg ttgcttgcga taccctcacg tgagttcata tgaccaacgc
     5221 tggcacgacc cctgcaggat caccagcgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     5281 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     5341 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     5401 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     5461 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     5521 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     5581 ttcgtggccg aggagcagga ctaagcagtc aggcagagtc gttgttgggt gtatggttgc
     5641 acaaagctag acctcgagct ggtcggttag aaagagatgc ttgtgcgact ggatgtaaca
     5701 ggcacaatcc gaggaaaatg caacagaaac gttatgatgt aacatctgag cacagtcaat
     5761 aaaattgcat gttgtatgcc gcacacaaaa gtgttgttaa acatgcgatg tggttgtcaa
     5821 atcattattc gcacaaccat ggaccgaacc tctggcacgc tgcatacgct gtattcattc
     5881 acatcatgtt ttctacttgt cacatgctct gcaccaagta actccgatta gttaggttcg
     5941 cacatgtcat cggagtggac cacttcgaat catttggttc gaagataaac cggatcccct
     6001 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     6061 atctctgatg ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg
     6121 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     6181 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     6241 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     6301 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     6361 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     6421 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     6481 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     6541 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     6601 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     6661 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     6721 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     6781 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     6841 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     6901 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     6961 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     7021 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     7081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7861 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7981 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     8041 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     8101 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     8161 agcgttcacc gacaaacaac agataaaacg aaaggcccag acttccgact gagcctttcg
     8221 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     8281 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gcccacc
//
