LOCUS       pGG-pUM140_0016-UM014_0107.1_TIC20-YFP-NOST-PIBT3        7488 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.1b-cgtoeij
VERSION     urn.local.jonasblomme.1b-cgtoeij
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             1970..2284
                     /note
                     /label="UM014_0107.1"
     CDS             2291..3010
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3015..3282
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          3287..3335
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3348..5174
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3348..3893
                     /label="UmpRbcS"
     CDS             join(3894..3901,4399..4765)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          3902..4398
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4766..5174
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(5179..5199)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        5260..5310
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5311..6120
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      6267..6855
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7454..7474
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tggttcccct
     1981 cgtgctgttt ttcctcgttt tcctggcaat tgtgaagaac aagcgactgc atcattttgt
     2041 ccggttcaat gcgatgcaag caatgatgct ggatattttg atcatgctgc caacagttgt
     2101 caatgcatac atccctgcag agctcttttg gacggtgctg ggcatgggct ttacctcaat
     2161 ctgcttcatg ggcatgttca tcgcacttgc atatgcagtc attttcacgc tggccgggaa
     2221 gtatgcggac atcccatttg tgtcggactc cgtgtacatg caggtttatc agatcgaatt
     2281 tttgtcagct atggtgagca agggcgagga gctgttcacg ggcgtggtgc ccatcctggt
     2341 ggagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2401 cgccacctac ggcaagctga ccctgaagtt catctgcacg accggcaagc tgcccgtgcc
     2461 ctggcccacc ctggtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga
     2521 ccacatgaag cagcacgact tcttcaagag cgccatgccg gagggctacg tgcaggagcg
     2581 caccatcttc ttcaaggacg acggcaacta caagacccgc gcggaggtga agttcgaggg
     2641 cgacacgctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2701 cctgggccac aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa
     2761 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctcggt
     2821 gcagctggcg gaccactacc agcagaacac ccccatcggc gacggcccgg tgctgctgcc
     2881 cgacaaccac tacctgtcct accagagcgc cctgtccaag gaccccaacg agaagcgcga
     2941 ccacatggtg ctgctggagt tcgtgaccgc cgccggcatc accctgggca tggacgagct
     3001 gtacaagtga ctgcctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta
     3061 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     3121 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     3181 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     3241 gataaattat cgcgcgcggt gtcatctatg ttactagatc gaactataag ctaggtagta
     3301 actagtgcga ctgtctagaa tcgcgtatgc gagtgtatga gctcgatcgt tgttcgtaca
     3361 caaggatgtc gcttgtggct ttttcgctcg gctttccagg agggcagtcc agataccatg
     3421 cggccgccgt gtccatcact cgcgctgcag ctgatgtggg cagatactgc actaatctgc
     3481 cgcacgacgc gtcggcctga gtcggcggac accggaggct tgctggagta ctggcacaag
     3541 tgtgcgtgtg attgccagaa acagcaaccc actcactaga tataccgctc caataatggg
     3601 ttcatgtgca agctcactca ataacgaaac gcttcactat tgtgcacgtg ccggggttgc
     3661 ttgggtatgg cgtgccatac gggttctgca catgtcaccc acgtgggtgt ggcacgagat
     3721 atcgaggtgg ccatgtaaaa ggcatttgct agagggcagc tcgtccatgc cgctcgagca
     3781 caccgagtgc gcaccataaa tgcagcacat gcctgtgcag cctatctcct gtctctgtta
     3841 catcccacca cctcctcaca tatccccttc cacccttaaa ccgcacagca gacatggcca
     3901 agtgcgcatc gcgtgtcaat gctggcgctg ctgacgtccg ctgcctaaac tgcatgaaag
     3961 ctcctgcagt cgcaagtagt ccttttaact tgttgtatcg gaacaaatgg ttgtagtctg
     4021 tgccgtgtga cgatcttgag ccgcctggtc ggcaggtcca atgtcggcct ggaccctgca
     4081 caccctgccc accctgccgc tgctcacccg ttcacgaccc cacaggtgct cgcagcagta
     4141 attgtcgata gatagcaact gtgttctgac tattctttga ccttccgcat ttgggactgc
     4201 ggcggcacac ctactatccg caatgcacac cctggtgctg gtactgcctg cctttccgcg
     4261 cgagcccaca ccatgcaggc gcatagtaat ggactgtgat tgatttaggc aactgtatga
     4321 aacctcttca tcgtgccttg gttgcttgcg ataccctcac gtgagttcat atgaccaacg
     4381 ctggcacgac ccctgcagga tcaccagcgc cgttccggtg ctcaccgcgc gcgacgtcgc
     4441 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     4501 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     4561 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     4621 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     4681 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     4741 cttcgtggcc gaggagcagg actaagcagt caggcagagt cgttgttggg tgtatggttg
     4801 cacaaagcta gacctcgagc tggtcggtta gaaagagatg cttgtgcgac tggatgtaac
     4861 aggcacaatc cgaggaaaat gcaacagaaa cgttatgatg taacatctga gcacagtcaa
     4921 taaaattgca tgttgtatgc cgcacacaaa agtgttgtta aacatgcgat gtggttgtca
     4981 aatcattatt cgcacaacca tggaccgaac ctctggcacg ctgcatacgc tgtattcatt
     5041 cacatcatgt tttctacttg tcacatgctc tgcaccaagt aactccgatt agttaggttc
     5101 gcacatgtca tcggagtgga ccacttcgaa tcatttggtt cgaagataaa ccggatcccc
     5161 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     5221 aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct
     5281 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     5341 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     5401 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     5461 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     5521 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     5581 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     5641 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     5701 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     5761 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     5821 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     5881 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     5941 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     6001 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     6061 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     6121 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     6181 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     6241 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6301 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6361 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6421 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6481 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6541 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6601 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6661 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6721 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6781 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6841 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6901 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6961 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7021 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7081 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7141 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     7201 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     7261 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     7321 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gacttccgac tgagcctttc
     7381 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     7441 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccacc
//
