LOCUS       pGG-pUM140_0016-UM003_0201.1_HistoneH3-YFP-NOST-PIBT3        7783 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.18-cgtoeih
VERSION     urn.local.jonasblomme.18-cgtoeih
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1970..2298,2389..2428,2541..2579)
                     /note
                     /label="UM003_0201.1"
     intron          2299..2388
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          2429..2540
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             2586..3305
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3310..3577
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          3582..3630
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3643..5469
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3643..4188
                     /label="UmpRbcS"
     CDS             join(4189..4196,4694..5060)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4197..4693
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      5061..5469
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(5474..5494)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        5555..5605
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5606..6415
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      6562..7150
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7749..7769
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tggctcgtac
     1981 caagcagacc gcacgtaagt ccacaggtgg caaggcccct cgtaagcagc tcgctaccaa
     2041 ggcagctcgt aagtcagccc ctgctacggg cggtgtcaag aagccccacc gctacaggcc
     2101 cggtaccgtc gctcttcgtg agatccgtaa gtaccagaag tctactgagc tcctcatccg
     2161 caagctgcct ttccagcggc tggtgcgtga gattgctcag gactacaaga cggacctgcg
     2221 tttccagagc tctgccgtcc tggcgctgca ggaggctgct gaggcctacc tggttggcct
     2281 gttcgaggac accaacttgt acgctctcaa tccggttgat atattttcaa ctttcaatgc
     2341 atttgtttgt attgagtgct gtatcatcaa gtcaacgttt gtgtgcaggt gtgctatcca
     2401 cgccaagcgc gtgactatca tgcccaaggt aagcttccgg cgataacaaa ctatcaaatt
     2461 tgtcttgtct cgagtttctt gtgtcggttg cctgtgaatg cgtctccgcg ccattctgac
     2521 cctttttcgt tgttttgcag gacgtccagc tcgcccgccg catccgtggt gagcgtgcct
     2581 cagctatggt gagcaagggc gaggagctgt tcacgggcgt ggtgcccatc ctggtggagc
     2641 tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgacgcca
     2701 cctacggcaa gctgaccctg aagttcatct gcacgaccgg caagctgccc gtgccctggc
     2761 ccaccctggt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     2821 tgaagcagca cgacttcttc aagagcgcca tgccggaggg ctacgtgcag gagcgcacca
     2881 tcttcttcaa ggacgacggc aactacaaga cccgcgcgga ggtgaagttc gagggcgaca
     2941 cgctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3001 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
     3061 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc tcggtgcagc
     3121 tggcggacca ctaccagcag aacaccccca tcggcgacgg cccggtgctg ctgcccgaca
     3181 accactacct gtcctaccag agcgccctgt ccaaggaccc caacgagaag cgcgaccaca
     3241 tggtgctgct ggagttcgtg accgccgccg gcatcaccct gggcatggac gagctgtaca
     3301 agtgactgcc tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt
     3361 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3421 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3481 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3541 attatcgcgc gcggtgtcat ctatgttact agatcgaact ataagctagg tagtaactag
     3601 tgcgactgtc tagaatcgcg tatgcgagtg tatgagctcg atcgttgttc gtacacaagg
     3661 atgtcgcttg tggctttttc gctcggcttt ccaggagggc agtccagata ccatgcggcc
     3721 gccgtgtcca tcactcgcgc tgcagctgat gtgggcagat actgcactaa tctgccgcac
     3781 gacgcgtcgg cctgagtcgg cggacaccgg aggcttgctg gagtactggc acaagtgtgc
     3841 gtgtgattgc cagaaacagc aacccactca ctagatatac cgctccaata atgggttcat
     3901 gtgcaagctc actcaataac gaaacgcttc actattgtgc acgtgccggg gttgcttggg
     3961 tatggcgtgc catacgggtt ctgcacatgt cacccacgtg ggtgtggcac gagatatcga
     4021 ggtggccatg taaaaggcat ttgctagagg gcagctcgtc catgccgctc gagcacaccg
     4081 agtgcgcacc ataaatgcag cacatgcctg tgcagcctat ctcctgtctc tgttacatcc
     4141 caccacctcc tcacatatcc ccttccaccc ttaaaccgca cagcagacat ggccaagtgc
     4201 gcatcgcgtg tcaatgctgg cgctgctgac gtccgctgcc taaactgcat gaaagctcct
     4261 gcagtcgcaa gtagtccttt taacttgttg tatcggaaca aatggttgta gtctgtgccg
     4321 tgtgacgatc ttgagccgcc tggtcggcag gtccaatgtc ggcctggacc ctgcacaccc
     4381 tgcccaccct gccgctgctc acccgttcac gaccccacag gtgctcgcag cagtaattgt
     4441 cgatagatag caactgtgtt ctgactattc tttgaccttc cgcatttggg actgcggcgg
     4501 cacacctact atccgcaatg cacaccctgg tgctggtact gcctgccttt ccgcgcgagc
     4561 ccacaccatg caggcgcata gtaatggact gtgattgatt taggcaactg tatgaaacct
     4621 cttcatcgtg ccttggttgc ttgcgatacc ctcacgtgag ttcatatgac caacgctggc
     4681 acgacccctg caggatcacc agcgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     4741 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     4801 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     4861 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     4921 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     4981 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     5041 tggccgagga gcaggactaa gcagtcaggc agagtcgttg ttgggtgtat ggttgcacaa
     5101 agctagacct cgagctggtc ggttagaaag agatgcttgt gcgactggat gtaacaggca
     5161 caatccgagg aaaatgcaac agaaacgtta tgatgtaaca tctgagcaca gtcaataaaa
     5221 ttgcatgttg tatgccgcac acaaaagtgt tgttaaacat gcgatgtggt tgtcaaatca
     5281 ttattcgcac aaccatggac cgaacctctg gcacgctgca tacgctgtat tcattcacat
     5341 catgttttct acttgtcaca tgctctgcac caagtaactc cgattagtta ggttcgcaca
     5401 tgtcatcgga gtggaccact tcgaatcatt tggttcgaag ataaaccgga tcccctatag
     5461 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     5521 ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta
     5581 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     5641 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     5701 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     5761 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     5821 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     5881 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     5941 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     6001 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     6061 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     6121 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     6181 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     6241 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     6301 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     6361 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     6421 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     6481 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     6541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7201 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7261 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7321 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7381 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7441 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     7501 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     7561 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     7621 ttcaccgaca aacaacagat aaaacgaaag gcccagactt ccgactgagc ctttcgtttt
     7681 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     7741 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc acc
//
