LOCUS pGG-pUM140_0016-mCherry-NOST-PIBT3 7185 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.85-cn3z25s
VERSION urn.local.jonasblomme.85-cn3z25s
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/.
FEATURES Location/Qualifiers
promoter 2..1935
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS 1997..2704
/SerialCloner_Color="&hFF0000"
/SerialCloner_Show="True"
/SerialCloner_Protect="True"
/SerialCloner_Arrow="True"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="mCherry"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; mCherry"
/modified_by="jonasblomme"
/label="mCherry"
terminator 2712..2979
/vntifkey=43
/modified_by="mamee"
/label="tNOS"
Linker 2984..3032
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
Plant_Selection 3045..4871
/created_by="mamee"
/modified_by="mamee"
/label="PIBT3"
promoter 3045..3590
/label="UmpRbcS"
CDS join(3591..3598,4096..4462)
/modified_by="jonasblomme"
/label="ble"
intron 3599..4095
/gene="ble"
/note="Ulva mutabilis RbcS intron 1; enhancer"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RbcsI"
/Transferred_Similarity="99.40%"
/Primary_Match="99.40%; Adjusted-Interval-0:0/0; RbcsI"
/modified_by="jonasblomme"
/label="RbcsI"
terminator 4463..4871
/modified_by="mamee"
/label="Ulva tRbcS"
primer complement(4876..4896)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 4957..5007
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 5008..5817
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 5964..6552
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 7151..7171
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tccagtcgac
1981 cggcaccaac tcagctatgg tgtccaaggg cgaggaggac aacatggcca tcatcaagga
2041 gttcatgcgc ttcaaggtgc acatggaggg cagcgtgaac ggccacgagt tcgagatcga
2101 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
2161 gggcggcccc ctgcccttcg cctgggacat cctgagcccc cagttcatgt acggcagcaa
2221 ggcctacgtg aagcaccccg ccgacatccc cgactacctg aagctgagct tccccgaggg
2281 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
2341 cagcagcctc caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
2401 cagcgacggc cccgtgatgc agaagaagac catgggctgg gaggccagca gcgagcgcat
2461 gtaccccgag gacggcgccc tgaagggcga gatcaagcag cgcctgaagc tgaaggacgg
2521 cggccactac gacgccgagg tgaagaccac ctacaaggcc aagaagcccg tgcagctgcc
2581 cggcgcctac aacgtgaaca tcaagctgga catcaccagc cacaacgagg actacaccat
2641 cgtggagcag tacgagcgcg ctgagggccg ccacagcacc ggcggcatgg acgagctgta
2701 caagtaactg cctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
2761 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
2821 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
2881 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
2941 aaattatcgc gcgcggtgtc atctatgtta ctagatcgaa ctataagcta ggtagtaact
3001 agtgcgactg tctagaatcg cgtatgcgag tgtatgagct cgatcgttgt tcgtacacaa
3061 ggatgtcgct tgtggctttt tcgctcggct ttccaggagg gcagtccaga taccatgcgg
3121 ccgccgtgtc catcactcgc gctgcagctg atgtgggcag atactgcact aatctgccgc
3181 acgacgcgtc ggcctgagtc ggcggacacc ggaggcttgc tggagtactg gcacaagtgt
3241 gcgtgtgatt gccagaaaca gcaacccact cactagatat accgctccaa taatgggttc
3301 atgtgcaagc tcactcaata acgaaacgct tcactattgt gcacgtgccg gggttgcttg
3361 ggtatggcgt gccatacggg ttctgcacat gtcacccacg tgggtgtggc acgagatatc
3421 gaggtggcca tgtaaaaggc atttgctaga gggcagctcg tccatgccgc tcgagcacac
3481 cgagtgcgca ccataaatgc agcacatgcc tgtgcagcct atctcctgtc tctgttacat
3541 cccaccacct cctcacatat ccccttccac ccttaaaccg cacagcagac atggccaagt
3601 gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg cctaaactgc atgaaagctc
3661 ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa caaatggttg tagtctgtgc
3721 cgtgtgacga tcttgagccg cctggtcggc aggtccaatg tcggcctgga ccctgcacac
3781 cctgcccacc ctgccgctgc tcacccgttc acgaccccac aggtgctcgc agcagtaatt
3841 gtcgatagat agcaactgtg ttctgactat tctttgacct tccgcatttg ggactgcggc
3901 ggcacaccta ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct ttccgcgcga
3961 gcccacacca tgcaggcgca tagtaatgga ctgtgattga tttaggcaac tgtatgaaac
4021 ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg agttcatatg accaacgctg
4081 gcacgacccc tgcaggatca ccagcgccgt tccggtgctc accgcgcgcg acgtcgccgg
4141 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
4201 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
4261 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
4321 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
4381 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
4441 cgtggccgag gagcaggact aagcagtcag gcagagtcgt tgttgggtgt atggttgcac
4501 aaagctagac ctcgagctgg tcggttagaa agagatgctt gtgcgactgg atgtaacagg
4561 cacaatccga ggaaaatgca acagaaacgt tatgatgtaa catctgagca cagtcaataa
4621 aattgcatgt tgtatgccgc acacaaaagt gttgttaaac atgcgatgtg gttgtcaaat
4681 cattattcgc acaaccatgg accgaacctc tggcacgctg catacgctgt attcattcac
4741 atcatgtttt ctacttgtca catgctctgc accaagtaac tccgattagt taggttcgca
4801 catgtcatcg gagtggacca cttcgaatca tttggttcga agataaaccg gatcccctat
4861 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
4921 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct
4981 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
5041 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
5101 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
5161 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
5221 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
5281 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
5341 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
5401 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
5461 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
5521 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
5581 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
5641 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
5701 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
5761 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
5821 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
5881 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
6601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
6661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
6721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
6781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
6841 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
6901 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
6961 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
7021 cgttcaccga caaacaacag ataaaacgaa aggcccagac ttccgactga gcctttcgtt
7081 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
7141 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccacc
//