LOCUS pGG-pUM140_0016-mCherry-NOST-PIBT3 7185 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.85-cn3z25s VERSION urn.local.jonasblomme.85-cn3z25s KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/. FEATURES Location/Qualifiers promoter 2..1935 /modified_by="jonasblomme" /label="pUM140_0016" CDS 1997..2704 /SerialCloner_Color="&hFF0000" /SerialCloner_Show="True" /SerialCloner_Protect="True" /SerialCloner_Arrow="True" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="mCherry" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mCherry" /modified_by="jonasblomme" /label="mCherry" terminator 2712..2979 /vntifkey=43 /modified_by="mamee" /label="tNOS" Linker 2984..3032 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" Plant_Selection 3045..4871 /created_by="mamee" /modified_by="mamee" /label="PIBT3" promoter 3045..3590 /label="UmpRbcS" CDS join(3591..3598,4096..4462) /modified_by="jonasblomme" /label="ble" intron 3599..4095 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RbcsI" /Transferred_Similarity="99.40%" /Primary_Match="99.40%; Adjusted-Interval-0:0/0; RbcsI" /modified_by="jonasblomme" /label="RbcsI" terminator 4463..4871 /modified_by="mamee" /label="Ulva tRbcS" primer complement(4876..4896) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 4957..5007 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 5008..5817 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 5964..6552 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 7151..7171 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca 61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg 121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg 181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag 241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca 301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt 361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt 421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct 481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta 541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt 601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt 661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag 721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat 781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg 841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg 901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg 961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag 1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga 1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg 1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg 1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg 1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct 1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag 1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc 1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt 1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat 1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc 1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg 1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa 1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt 1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc 1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc 1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tccagtcgac 1981 cggcaccaac tcagctatgg tgtccaaggg cgaggaggac aacatggcca tcatcaagga 2041 gttcatgcgc ttcaaggtgc acatggaggg cagcgtgaac ggccacgagt tcgagatcga 2101 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 2161 gggcggcccc ctgcccttcg cctgggacat cctgagcccc cagttcatgt acggcagcaa 2221 ggcctacgtg aagcaccccg ccgacatccc cgactacctg aagctgagct tccccgaggg 2281 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 2341 cagcagcctc caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 2401 cagcgacggc cccgtgatgc agaagaagac catgggctgg gaggccagca gcgagcgcat 2461 gtaccccgag gacggcgccc tgaagggcga gatcaagcag cgcctgaagc tgaaggacgg 2521 cggccactac gacgccgagg tgaagaccac ctacaaggcc aagaagcccg tgcagctgcc 2581 cggcgcctac aacgtgaaca tcaagctgga catcaccagc cacaacgagg actacaccat 2641 cgtggagcag tacgagcgcg ctgagggccg ccacagcacc ggcggcatgg acgagctgta 2701 caagtaactg cctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga 2761 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag 2821 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga 2881 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat 2941 aaattatcgc gcgcggtgtc atctatgtta ctagatcgaa ctataagcta ggtagtaact 3001 agtgcgactg tctagaatcg cgtatgcgag tgtatgagct cgatcgttgt tcgtacacaa 3061 ggatgtcgct tgtggctttt tcgctcggct ttccaggagg gcagtccaga taccatgcgg 3121 ccgccgtgtc catcactcgc gctgcagctg atgtgggcag atactgcact aatctgccgc 3181 acgacgcgtc ggcctgagtc ggcggacacc ggaggcttgc tggagtactg gcacaagtgt 3241 gcgtgtgatt gccagaaaca gcaacccact cactagatat accgctccaa taatgggttc 3301 atgtgcaagc tcactcaata acgaaacgct tcactattgt gcacgtgccg gggttgcttg 3361 ggtatggcgt gccatacggg ttctgcacat gtcacccacg tgggtgtggc acgagatatc 3421 gaggtggcca tgtaaaaggc atttgctaga gggcagctcg tccatgccgc tcgagcacac 3481 cgagtgcgca ccataaatgc agcacatgcc tgtgcagcct atctcctgtc tctgttacat 3541 cccaccacct cctcacatat ccccttccac ccttaaaccg cacagcagac atggccaagt 3601 gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg cctaaactgc atgaaagctc 3661 ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa caaatggttg tagtctgtgc 3721 cgtgtgacga tcttgagccg cctggtcggc aggtccaatg tcggcctgga ccctgcacac 3781 cctgcccacc ctgccgctgc tcacccgttc acgaccccac aggtgctcgc agcagtaatt 3841 gtcgatagat agcaactgtg ttctgactat tctttgacct tccgcatttg ggactgcggc 3901 ggcacaccta ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct ttccgcgcga 3961 gcccacacca tgcaggcgca tagtaatgga ctgtgattga tttaggcaac tgtatgaaac 4021 ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg agttcatatg accaacgctg 4081 gcacgacccc tgcaggatca ccagcgccgt tccggtgctc accgcgcgcg acgtcgccgg 4141 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt 4201 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt 4261 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg 4321 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg 4381 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt 4441 cgtggccgag gagcaggact aagcagtcag gcagagtcgt tgttgggtgt atggttgcac 4501 aaagctagac ctcgagctgg tcggttagaa agagatgctt gtgcgactgg atgtaacagg 4561 cacaatccga ggaaaatgca acagaaacgt tatgatgtaa catctgagca cagtcaataa 4621 aattgcatgt tgtatgccgc acacaaaagt gttgttaaac atgcgatgtg gttgtcaaat 4681 cattattcgc acaaccatgg accgaacctc tggcacgctg catacgctgt attcattcac 4741 atcatgtttt ctacttgtca catgctctgc accaagtaac tccgattagt taggttcgca 4801 catgtcatcg gagtggacca cttcgaatca tttggttcga agataaaccg gatcccctat 4861 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat 4921 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct 4981 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 5041 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 5101 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 5161 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 5221 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 5281 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 5341 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 5401 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 5461 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 5521 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 5581 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 5641 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 5701 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 5761 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 5821 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca 5881 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc 5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 6601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 6661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 6721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 6781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 6841 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga 6901 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc 6961 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag 7021 cgttcaccga caaacaacag ataaaacgaa aggcccagac ttccgactga gcctttcgtt 7081 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag 7141 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccacc //