LOCUS       pGG-pUM140_0016-mTagBFP-NOST-PIBT3        7176 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.84-cn3z25s
VERSION     urn.local.jonasblomme.84-cn3z25s
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             1997..2695
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mTagBFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mTagBFP
                     (<a
                     href=""urn:local:jonasblomme:2d-cnsqc2r"">mTagBFP2</a>)"
                     /modified_by="jonasblomme"
                     /label="mTagBFP"
     terminator      2703..2970
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          2975..3023
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3036..4862
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3036..3581
                     /label="UmpRbcS"
     CDS             join(3582..3589,4087..4453)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          3590..4086
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4454..4862
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(4867..4887)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4948..4998
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4999..5808
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5955..6543
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7142..7162
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tccagtcgac
     1981 cggcaccaac tcagctatga gcgagctgat caaggagaac atgcacatga agctgtacat
     2041 ggagggcacc gtggacaacc accacttcaa gtgcaccagc gagggcgagg gcaagcccta
     2101 cgagggcacc cagaccatgc gcatcaaggt ggtggagggc ggccccctgc ccttcgcctt
     2161 cgacatcctg gccacctcgt tcctgtacgg cagcaagacc ttcatcaacc acacccaggg
     2221 catccccgac ttcttcaagc agagcttccc cgagggcttc acctgggagc gcgtgaccac
     2281 ctacgaggac ggcggcgtgc tgaccgccac ccaggacacc agcctccagg acggctgcct
     2341 gatctacaac gtgaagatcc gcggcgtgaa cttcaccagc aacggccccg tgatgcagaa
     2401 gaagaccctg ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
     2461 gggccgcaac gacatggccc tgaagctggt gggcggcagc cacctgatcg ccaacatcaa
     2521 gaccacctac cgcagcaaga agcccgccaa gaacctgaag atgcccggcg tgtactacgt
     2581 ggactaccgc ctggagcgca tcaaggaggc caacaacgag acgtacgtgg agcagcacga
     2641 ggtggccgtg gcccgctact gcgacctgcc cagcaagctg ggccacaagc tgaactaact
     2701 gcctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     2761 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     2821 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     2881 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     2941 cgcgcggtgt catctatgtt actagatcga actataagct aggtagtaac tagtgcgact
     3001 gtctagaatc gcgtatgcga gtgtatgagc tcgatcgttg ttcgtacaca aggatgtcgc
     3061 ttgtggcttt ttcgctcggc tttccaggag ggcagtccag ataccatgcg gccgccgtgt
     3121 ccatcactcg cgctgcagct gatgtgggca gatactgcac taatctgccg cacgacgcgt
     3181 cggcctgagt cggcggacac cggaggcttg ctggagtact ggcacaagtg tgcgtgtgat
     3241 tgccagaaac agcaacccac tcactagata taccgctcca ataatgggtt catgtgcaag
     3301 ctcactcaat aacgaaacgc ttcactattg tgcacgtgcc ggggttgctt gggtatggcg
     3361 tgccatacgg gttctgcaca tgtcacccac gtgggtgtgg cacgagatat cgaggtggcc
     3421 atgtaaaagg catttgctag agggcagctc gtccatgccg ctcgagcaca ccgagtgcgc
     3481 accataaatg cagcacatgc ctgtgcagcc tatctcctgt ctctgttaca tcccaccacc
     3541 tcctcacata tccccttcca cccttaaacc gcacagcaga catggccaag tgcgcatcgc
     3601 gtgtcaatgc tggcgctgct gacgtccgct gcctaaactg catgaaagct cctgcagtcg
     3661 caagtagtcc ttttaacttg ttgtatcgga acaaatggtt gtagtctgtg ccgtgtgacg
     3721 atcttgagcc gcctggtcgg caggtccaat gtcggcctgg accctgcaca ccctgcccac
     3781 cctgccgctg ctcacccgtt cacgacccca caggtgctcg cagcagtaat tgtcgataga
     3841 tagcaactgt gttctgacta ttctttgacc ttccgcattt gggactgcgg cggcacacct
     3901 actatccgca atgcacaccc tggtgctggt actgcctgcc tttccgcgcg agcccacacc
     3961 atgcaggcgc atagtaatgg actgtgattg atttaggcaa ctgtatgaaa cctcttcatc
     4021 gtgccttggt tgcttgcgat accctcacgt gagttcatat gaccaacgct ggcacgaccc
     4081 ctgcaggatc accagcgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     4141 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     4201 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     4261 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     4321 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     4381 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     4441 ggagcaggac taagcagtca ggcagagtcg ttgttgggtg tatggttgca caaagctaga
     4501 cctcgagctg gtcggttaga aagagatgct tgtgcgactg gatgtaacag gcacaatccg
     4561 aggaaaatgc aacagaaacg ttatgatgta acatctgagc acagtcaata aaattgcatg
     4621 ttgtatgccg cacacaaaag tgttgttaaa catgcgatgt ggttgtcaaa tcattattcg
     4681 cacaaccatg gaccgaacct ctggcacgct gcatacgctg tattcattca catcatgttt
     4741 tctacttgtc acatgctctg caccaagtaa ctccgattag ttaggttcgc acatgtcatc
     4801 ggagtggacc acttcgaatc atttggttcg aagataaacc ggatccccta tagtgagtcg
     4861 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     4921 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac
     4981 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     5041 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     5101 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     5161 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     5221 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     5281 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     5341 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     5401 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     5461 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     5521 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     5581 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     5641 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     5701 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     5761 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     5821 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     5881 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     6841 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     6901 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     6961 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     7021 acaaacaaca gataaaacga aaggcccaga cttccgactg agcctttcgt tttatttgat
     7081 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     7141 tgtaaaacga cggccagtct taagctcggg cccacc
//
