LOCUS       pGG-pUM140_0016-YFP-NOST-PIBT3        7194 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.83-cn3z25s
VERSION     urn.local.jonasblomme.83-cn3z25s
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             1997..2716
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      2721..2988
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          2993..3041
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3054..4880
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3054..3599
                     /label="UmpRbcS"
     CDS             join(3600..3607,4105..4471)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          3608..4104
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4472..4880
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(4885..4905)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4966..5016
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5017..5826
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5973..6561
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7160..7180
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tccagtcgac
     1981 cggcaccaac tcagctatgg tgagcaaggg cgaggagctg ttcacgggcg tggtgcccat
     2041 cctggtggag ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga
     2101 gggcgacgcc acctacggca agctgaccct gaagttcatc tgcacgaccg gcaagctgcc
     2161 cgtgccctgg cccaccctgg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta
     2221 ccccgaccac atgaagcagc acgacttctt caagagcgcc atgccggagg gctacgtgca
     2281 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgcgg aggtgaagtt
     2341 cgagggcgac acgctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2401 caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaacgtgt acatcatggc
     2461 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2521 ctcggtgcag ctggcggacc actaccagca gaacaccccc atcggcgacg gcccggtgct
     2581 gctgcccgac aaccactacc tgtcctacca gagcgccctg tccaaggacc ccaacgagaa
     2641 gcgcgaccac atggtgctgc tggagttcgt gaccgccgcc ggcatcaccc tgggcatgga
     2701 cgagctgtac aagtgactgc ctagagtcaa gcagatcgtt caaacatttg gcaataaagt
     2761 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2821 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2881 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2941 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgaac tataagctag
     3001 gtagtaacta gtgcgactgt ctagaatcgc gtatgcgagt gtatgagctc gatcgttgtt
     3061 cgtacacaag gatgtcgctt gtggcttttt cgctcggctt tccaggaggg cagtccagat
     3121 accatgcggc cgccgtgtcc atcactcgcg ctgcagctga tgtgggcaga tactgcacta
     3181 atctgccgca cgacgcgtcg gcctgagtcg gcggacaccg gaggcttgct ggagtactgg
     3241 cacaagtgtg cgtgtgattg ccagaaacag caacccactc actagatata ccgctccaat
     3301 aatgggttca tgtgcaagct cactcaataa cgaaacgctt cactattgtg cacgtgccgg
     3361 ggttgcttgg gtatggcgtg ccatacgggt tctgcacatg tcacccacgt gggtgtggca
     3421 cgagatatcg aggtggccat gtaaaaggca tttgctagag ggcagctcgt ccatgccgct
     3481 cgagcacacc gagtgcgcac cataaatgca gcacatgcct gtgcagccta tctcctgtct
     3541 ctgttacatc ccaccacctc ctcacatatc cccttccacc cttaaaccgc acagcagaca
     3601 tggccaagtg cgcatcgcgt gtcaatgctg gcgctgctga cgtccgctgc ctaaactgca
     3661 tgaaagctcc tgcagtcgca agtagtcctt ttaacttgtt gtatcggaac aaatggttgt
     3721 agtctgtgcc gtgtgacgat cttgagccgc ctggtcggca ggtccaatgt cggcctggac
     3781 cctgcacacc ctgcccaccc tgccgctgct cacccgttca cgaccccaca ggtgctcgca
     3841 gcagtaattg tcgatagata gcaactgtgt tctgactatt ctttgacctt ccgcatttgg
     3901 gactgcggcg gcacacctac tatccgcaat gcacaccctg gtgctggtac tgcctgcctt
     3961 tccgcgcgag cccacaccat gcaggcgcat agtaatggac tgtgattgat ttaggcaact
     4021 gtatgaaacc tcttcatcgt gccttggttg cttgcgatac cctcacgtga gttcatatga
     4081 ccaacgctgg cacgacccct gcaggatcac cagcgccgtt ccggtgctca ccgcgcgcga
     4141 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     4201 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     4261 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     4321 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     4381 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     4441 cgtgcacttc gtggccgagg agcaggacta agcagtcagg cagagtcgtt gttgggtgta
     4501 tggttgcaca aagctagacc tcgagctggt cggttagaaa gagatgcttg tgcgactgga
     4561 tgtaacaggc acaatccgag gaaaatgcaa cagaaacgtt atgatgtaac atctgagcac
     4621 agtcaataaa attgcatgtt gtatgccgca cacaaaagtg ttgttaaaca tgcgatgtgg
     4681 ttgtcaaatc attattcgca caaccatgga ccgaacctct ggcacgctgc atacgctgta
     4741 ttcattcaca tcatgttttc tacttgtcac atgctctgca ccaagtaact ccgattagtt
     4801 aggttcgcac atgtcatcgg agtggaccac ttcgaatcat ttggttcgaa gataaaccgg
     4861 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     4921 tctcaaaatc tctgatgtta cattgcacaa gataaaataa tatcatcatg aacaataaaa
     4981 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     5041 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     5101 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     5161 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     5221 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     5281 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     5341 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     5401 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     5461 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     5521 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     5581 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     5641 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     5701 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     5761 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     5821 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     5881 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     5941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6601 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6661 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6721 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6781 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6841 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     6901 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     6961 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     7021 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagact tccgactgag
     7081 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     7141 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc cacc
//
