LOCUS       pGG-pUM140_0016-RbcsI-mTagBFP-NOST-PIBT3        7649 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.82-cn3z25s
VERSION     urn.local.jonasblomme.82-cn3z25s
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     intron          1936..2436
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     CDS             2470..3168
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mTagBFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mTagBFP
                     (<a
                     href=""urn:local:jonasblomme:2d-cnsqc2r"">mTagBFP2</a>)"
                     /modified_by="jonasblomme"
                     /label="mTagBFP"
     terminator      3176..3443
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          3448..3496
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3509..5335
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3509..4054
                     /label="UmpRbcS"
     CDS             join(4055..4062,4560..4926)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4063..4559
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4927..5335
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(5340..5360)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        5421..5471
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5472..6281
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      6428..7016
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7615..7635
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
     1981 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
     2041 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
     2101 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca
     2161 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc
     2221 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt
     2281 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg
     2341 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt
     2401 gagttcatat gaccaacgct ggcacgaccc ctgcagggct ccatccagtc gaccggcacc
     2461 aactcagcta tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc
     2521 accgtggaca accaccactt caagtgcacc agcgagggcg agggcaagcc ctacgagggc
     2581 acccagacca tgcgcatcaa ggtggtggag ggcggccccc tgcccttcgc cttcgacatc
     2641 ctggccacct cgttcctgta cggcagcaag accttcatca accacaccca gggcatcccc
     2701 gacttcttca agcagagctt ccccgagggc ttcacctggg agcgcgtgac cacctacgag
     2761 gacggcggcg tgctgaccgc cacccaggac accagcctcc aggacggctg cctgatctac
     2821 aacgtgaaga tccgcggcgt gaacttcacc agcaacggcc ccgtgatgca gaagaagacc
     2881 ctgggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggagggccgc
     2941 aacgacatgg ccctgaagct ggtgggcggc agccacctga tcgccaacat caagaccacc
     3001 taccgcagca agaagcccgc caagaacctg aagatgcccg gcgtgtacta cgtggactac
     3061 cgcctggagc gcatcaagga ggccaacaac gagacgtacg tggagcagca cgaggtggcc
     3121 gtggcccgct actgcgacct gcccagcaag ctgggccaca agctgaacta actgcctaga
     3181 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     3241 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     3301 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     3361 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     3421 tgtcatctat gttactagat cgaactataa gctaggtagt aactagtgcg actgtctaga
     3481 atcgcgtatg cgagtgtatg agctcgatcg ttgttcgtac acaaggatgt cgcttgtggc
     3541 tttttcgctc ggctttccag gagggcagtc cagataccat gcggccgccg tgtccatcac
     3601 tcgcgctgca gctgatgtgg gcagatactg cactaatctg ccgcacgacg cgtcggcctg
     3661 agtcggcgga caccggaggc ttgctggagt actggcacaa gtgtgcgtgt gattgccaga
     3721 aacagcaacc cactcactag atataccgct ccaataatgg gttcatgtgc aagctcactc
     3781 aataacgaaa cgcttcacta ttgtgcacgt gccggggttg cttgggtatg gcgtgccata
     3841 cgggttctgc acatgtcacc cacgtgggtg tggcacgaga tatcgaggtg gccatgtaaa
     3901 aggcatttgc tagagggcag ctcgtccatg ccgctcgagc acaccgagtg cgcaccataa
     3961 atgcagcaca tgcctgtgca gcctatctcc tgtctctgtt acatcccacc acctcctcac
     4021 atatcccctt ccacccttaa accgcacagc agacatggcc aagtgcgcat cgcgtgtcaa
     4081 tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag tcgcaagtag
     4141 tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg acgatcttga
     4201 gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc caccctgccg
     4261 ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat agatagcaac
     4321 tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca cctactatcc
     4381 gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac accatgcagg
     4441 cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc atcgtgcctt
     4501 ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga cccctgcagg
     4561 atcaccagcg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     4621 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     4681 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     4741 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     4801 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     4861 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     4921 gactaagcag tcaggcagag tcgttgttgg gtgtatggtt gcacaaagct agacctcgag
     4981 ctggtcggtt agaaagagat gcttgtgcga ctggatgtaa caggcacaat ccgaggaaaa
     5041 tgcaacagaa acgttatgat gtaacatctg agcacagtca ataaaattgc atgttgtatg
     5101 ccgcacacaa aagtgttgtt aaacatgcga tgtggttgtc aaatcattat tcgcacaacc
     5161 atggaccgaa cctctggcac gctgcatacg ctgtattcat tcacatcatg ttttctactt
     5221 gtcacatgct ctgcaccaag taactccgat tagttaggtt cgcacatgtc atcggagtgg
     5281 accacttcga atcatttggt tcgaagataa accggatccc ctatagtgag tcgtattaca
     5341 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     5401 cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     5461 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     5521 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     5581 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     5641 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     5701 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     5761 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     5821 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     5881 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     5941 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     6001 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     6061 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     6121 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     6181 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     6241 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     6301 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     6361 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7081 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7141 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7201 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7261 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     7321 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     7381 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     7441 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     7501 acagataaaa cgaaaggccc agacttccga ctgagccttt cgttttattt gatgcctggc
     7561 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     7621 cgacggccag tcttaagctc gggcccacc
//
