LOCUS       pGG-pUM140_0016-RbcsI-mCherry-NOST-PIBT3        7658 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.81-cn3z25s
VERSION     urn.local.jonasblomme.81-cn3z25s
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     intron          1936..2436
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     CDS             2470..3177
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:2i-cnsqdta"">mCherry</a>"
                     /modified_by="jonasblomme"
                     /label="mCherry"
     terminator      3185..3452
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          3457..3505
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3518..5344
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3518..4063
                     /label="UmpRbcS"
     CDS             join(4064..4071,4569..4935)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4072..4568
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4936..5344
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(5349..5369)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        5430..5480
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5481..6290
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      6437..7025
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7624..7644
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
     1981 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
     2041 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
     2101 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca
     2161 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc
     2221 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt
     2281 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg
     2341 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt
     2401 gagttcatat gaccaacgct ggcacgaccc ctgcagggct ccatccagtc gaccggcacc
     2461 aactcagcta tggtgtccaa gggcgaggag gacaacatgg ccatcatcaa ggagttcatg
     2521 cgcttcaagg tgcacatgga gggcagcgtg aacggccacg agttcgagat cgagggcgag
     2581 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     2641 cccctgccct tcgcctggga catcctgagc ccccagttca tgtacggcag caaggcctac
     2701 gtgaagcacc ccgccgacat ccccgactac ctgaagctga gcttccccga gggcttcaag
     2761 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggacagcagc
     2821 ctccaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt ccccagcgac
     2881 ggccccgtga tgcagaagaa gaccatgggc tgggaggcca gcagcgagcg catgtacccc
     2941 gaggacggcg ccctgaaggg cgagatcaag cagcgcctga agctgaagga cggcggccac
     3001 tacgacgccg aggtgaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3061 tacaacgtga acatcaagct ggacatcacc agccacaacg aggactacac catcgtggag
     3121 cagtacgagc gcgctgaggg ccgccacagc accggcggca tggacgagct gtacaagtaa
     3181 ctgcctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3241 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     3301 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     3361 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     3421 cgcgcgcggt gtcatctatg ttactagatc gaactataag ctaggtagta actagtgcga
     3481 ctgtctagaa tcgcgtatgc gagtgtatga gctcgatcgt tgttcgtaca caaggatgtc
     3541 gcttgtggct ttttcgctcg gctttccagg agggcagtcc agataccatg cggccgccgt
     3601 gtccatcact cgcgctgcag ctgatgtggg cagatactgc actaatctgc cgcacgacgc
     3661 gtcggcctga gtcggcggac accggaggct tgctggagta ctggcacaag tgtgcgtgtg
     3721 attgccagaa acagcaaccc actcactaga tataccgctc caataatggg ttcatgtgca
     3781 agctcactca ataacgaaac gcttcactat tgtgcacgtg ccggggttgc ttgggtatgg
     3841 cgtgccatac gggttctgca catgtcaccc acgtgggtgt ggcacgagat atcgaggtgg
     3901 ccatgtaaaa ggcatttgct agagggcagc tcgtccatgc cgctcgagca caccgagtgc
     3961 gcaccataaa tgcagcacat gcctgtgcag cctatctcct gtctctgtta catcccacca
     4021 cctcctcaca tatccccttc cacccttaaa ccgcacagca gacatggcca agtgcgcatc
     4081 gcgtgtcaat gctggcgctg ctgacgtccg ctgcctaaac tgcatgaaag ctcctgcagt
     4141 cgcaagtagt ccttttaact tgttgtatcg gaacaaatgg ttgtagtctg tgccgtgtga
     4201 cgatcttgag ccgcctggtc ggcaggtcca atgtcggcct ggaccctgca caccctgccc
     4261 accctgccgc tgctcacccg ttcacgaccc cacaggtgct cgcagcagta attgtcgata
     4321 gatagcaact gtgttctgac tattctttga ccttccgcat ttgggactgc ggcggcacac
     4381 ctactatccg caatgcacac cctggtgctg gtactgcctg cctttccgcg cgagcccaca
     4441 ccatgcaggc gcatagtaat ggactgtgat tgatttaggc aactgtatga aacctcttca
     4501 tcgtgccttg gttgcttgcg ataccctcac gtgagttcat atgaccaacg ctggcacgac
     4561 ccctgcagga tcaccagcgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     4621 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     4681 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     4741 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     4801 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     4861 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     4921 gaggagcagg actaagcagt caggcagagt cgttgttggg tgtatggttg cacaaagcta
     4981 gacctcgagc tggtcggtta gaaagagatg cttgtgcgac tggatgtaac aggcacaatc
     5041 cgaggaaaat gcaacagaaa cgttatgatg taacatctga gcacagtcaa taaaattgca
     5101 tgttgtatgc cgcacacaaa agtgttgtta aacatgcgat gtggttgtca aatcattatt
     5161 cgcacaacca tggaccgaac ctctggcacg ctgcatacgc tgtattcatt cacatcatgt
     5221 tttctacttg tcacatgctc tgcaccaagt aactccgatt agttaggttc gcacatgtca
     5281 tcggagtgga ccacttcgaa tcatttggtt cgaagataaa ccggatcccc tatagtgagt
     5341 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     5401 gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa
     5461 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     5521 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     5581 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     5641 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     5701 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     5761 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     5821 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     5881 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     5941 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     6001 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     6061 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     6121 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     6181 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     6241 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     6301 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     6361 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     6421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7081 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7141 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7201 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7261 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     7321 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     7381 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     7441 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     7501 cgacaaacaa cagataaaac gaaaggccca gacttccgac tgagcctttc gttttatttg
     7561 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     7621 gttgtaaaac gacggccagt cttaagctcg ggcccacc
//
