LOCUS       pGG-pUM140_0016-RbcsI-YFP-NOST-PIBT3        7667 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.80-cn3z25s
VERSION     urn.local.jonasblomme.80-cn3z25s
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     intron          1936..2436
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     CDS             2470..3189
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3194..3461
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          3466..3514
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3527..5353
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3527..4072
                     /label="UmpRbcS"
     CDS             join(4073..4080,4578..4944)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4081..4577
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4945..5353
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(5358..5378)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        5439..5489
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5490..6299
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      6446..7034
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7633..7653
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
     1981 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
     2041 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
     2101 gtcggcctgg accctgcaca ccctgcccac cctgccgctg ctcacccgtt cacgacccca
     2161 caggtgctcg cagcagtaat tgtcgataga tagcaactgt gttctgacta ttctttgacc
     2221 ttccgcattt gggactgcgg cggcacacct actatccgca atgcacaccc tggtgctggt
     2281 actgcctgcc tttccgcgcg agcccacacc atgcaggcgc atagtaatgg actgtgattg
     2341 atttaggcaa ctgtatgaaa cctcttcatc gtgccttggt tgcttgcgat accctcacgt
     2401 gagttcatat gaccaacgct ggcacgaccc ctgcagggct ccatccagtc gaccggcacc
     2461 aactcagcta tggtgagcaa gggcgaggag ctgttcacgg gcgtggtgcc catcctggtg
     2521 gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgac
     2581 gccacctacg gcaagctgac cctgaagttc atctgcacga ccggcaagct gcccgtgccc
     2641 tggcccaccc tggtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac
     2701 cacatgaagc agcacgactt cttcaagagc gccatgccgg agggctacgt gcaggagcgc
     2761 accatcttct tcaaggacga cggcaactac aagacccgcg cggaggtgaa gttcgagggc
     2821 gacacgctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2881 ctgggccaca agctggagta caactacaac agccacaacg tgtacatcat ggccgacaag
     2941 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggctcggtg
     3001 cagctggcgg accactacca gcagaacacc cccatcggcg acggcccggt gctgctgccc
     3061 gacaaccact acctgtccta ccagagcgcc ctgtccaagg accccaacga gaagcgcgac
     3121 cacatggtgc tgctggagtt cgtgaccgcc gccggcatca ccctgggcat ggacgagctg
     3181 tacaagtgac tgcctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa
     3241 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     3301 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     3361 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     3421 ataaattatc gcgcgcggtg tcatctatgt tactagatcg aactataagc taggtagtaa
     3481 ctagtgcgac tgtctagaat cgcgtatgcg agtgtatgag ctcgatcgtt gttcgtacac
     3541 aaggatgtcg cttgtggctt tttcgctcgg ctttccagga gggcagtcca gataccatgc
     3601 ggccgccgtg tccatcactc gcgctgcagc tgatgtgggc agatactgca ctaatctgcc
     3661 gcacgacgcg tcggcctgag tcggcggaca ccggaggctt gctggagtac tggcacaagt
     3721 gtgcgtgtga ttgccagaaa cagcaaccca ctcactagat ataccgctcc aataatgggt
     3781 tcatgtgcaa gctcactcaa taacgaaacg cttcactatt gtgcacgtgc cggggttgct
     3841 tgggtatggc gtgccatacg ggttctgcac atgtcaccca cgtgggtgtg gcacgagata
     3901 tcgaggtggc catgtaaaag gcatttgcta gagggcagct cgtccatgcc gctcgagcac
     3961 accgagtgcg caccataaat gcagcacatg cctgtgcagc ctatctcctg tctctgttac
     4021 atcccaccac ctcctcacat atccccttcc acccttaaac cgcacagcag acatggccaa
     4081 gtgcgcatcg cgtgtcaatg ctggcgctgc tgacgtccgc tgcctaaact gcatgaaagc
     4141 tcctgcagtc gcaagtagtc cttttaactt gttgtatcgg aacaaatggt tgtagtctgt
     4201 gccgtgtgac gatcttgagc cgcctggtcg gcaggtccaa tgtcggcctg gaccctgcac
     4261 accctgccca ccctgccgct gctcacccgt tcacgacccc acaggtgctc gcagcagtaa
     4321 ttgtcgatag atagcaactg tgttctgact attctttgac cttccgcatt tgggactgcg
     4381 gcggcacacc tactatccgc aatgcacacc ctggtgctgg tactgcctgc ctttccgcgc
     4441 gagcccacac catgcaggcg catagtaatg gactgtgatt gatttaggca actgtatgaa
     4501 acctcttcat cgtgccttgg ttgcttgcga taccctcacg tgagttcata tgaccaacgc
     4561 tggcacgacc cctgcaggat caccagcgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     4621 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     4681 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     4741 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     4801 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     4861 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     4921 ttcgtggccg aggagcagga ctaagcagtc aggcagagtc gttgttgggt gtatggttgc
     4981 acaaagctag acctcgagct ggtcggttag aaagagatgc ttgtgcgact ggatgtaaca
     5041 ggcacaatcc gaggaaaatg caacagaaac gttatgatgt aacatctgag cacagtcaat
     5101 aaaattgcat gttgtatgcc gcacacaaaa gtgttgttaa acatgcgatg tggttgtcaa
     5161 atcattattc gcacaaccat ggaccgaacc tctggcacgc tgcatacgct gtattcattc
     5221 acatcatgtt ttctacttgt cacatgctct gcaccaagta actccgatta gttaggttcg
     5281 cacatgtcat cggagtggac cacttcgaat catttggttc gaagataaac cggatcccct
     5341 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     5401 atctctgatg ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg
     5461 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     5521 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     5581 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     5641 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     5701 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     5761 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     5821 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     5881 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     5941 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     6001 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     6061 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     6121 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     6181 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     6241 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     6301 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     6361 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7201 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7261 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7321 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     7381 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     7441 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     7501 agcgttcacc gacaaacaac agataaaacg aaaggcccag acttccgact gagcctttcg
     7561 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     7621 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gcccacc
//
