LOCUS       pGG-UmpRbcS_446-bleGFP-tRbcS        4906 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6r-cgtp4xy
VERSION     urn.local.jonasblomme.6r-cgtp4xy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..446
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /modified_by="jonasblomme"
                     /label="UmpRbcS"
     CDS             join(481..488,986..2063)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          489..985
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          2068..2113
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      2118..2506
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2511..2559
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2596..2616)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2677..2727
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2728..3537
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3684..4272
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4871..4891
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cagctgatgt gggcagatac tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg
       61 gacaccggag gcttgctgga gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa
      121 cccactcact agatataccg ctccaataat gggttcatgt gcaagctcac tcaataacga
      181 aacgcttcac tattgtgcac gtgccggggt tgcttgggta tggcgtgcca tacgggttct
      241 gcacatgtca cccacgtggg tgtggcacga gatatcgagg tggccatgta aaaggcattt
      301 gctagagggc agctcgtcca tgccgctcga gcacaccgag tgcgcaccat aaatgcagca
      361 catgcctgtg cagcctatct cctgtctctg ttacatccca ccacctcctc acatatcccc
      421 ttccaccctt aaaccgcaca gcagacaaca gtattcagtc gactggtacc aacaggctcc
      481 atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg cctaaactgc
      541 atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa caaatggttg
      601 tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg tcggcctgga
      661 ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac aggtgctcgc
      721 agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct tccgcatttg
      781 ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct
      841 ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga tttaggcaac
      901 tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg agttcatatg
      961 accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc accgcgcgcg
     1021 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     1081 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     1141 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     1201 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     1261 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     1321 gcgtgcactt cgtggccgag gagcaggtac ccaagggcga ggagctgttc accggtgtgg
     1381 tccccatcct ggtggagctg gacggcgacg tgaacggcca caagttctcc gtctccggcg
     1441 agggtgaggg tgacgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1501 agctgcccgt gccctggccc accctggtca ccaccctgac ctacggtgtg cagtgcttct
     1561 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgagggct
     1621 acgtgcagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1681 tcaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1741 aggacggcaa catcctgggc cacaagctgg agtacaacta caactcccac aacgtgtaca
     1801 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1861 aggacggctc cgtgcagctg gccgaccact accagcagaa cacccccatc ggcgatggcc
     1921 ccgtgctgct gcccgacaac cactacctgt ccatccagtc cgccctgtcc aaggacccca
     1981 acgagaagcg cgaccacatg gtcctgctgg agttcgtcac cgctgccggc atcacccacg
     2041 gcatggacga gctgtacaag taatcaggtg gatcctagat aacctttacc ttcattccct
     2101 gtaaattggt accctgcgca gtcaggcaga gtcgttgttg ggtgtatggt tgcacaaaac
     2161 tagacctcga gctggtcggt tagaaagaga tgcttgtgcg actggatgta acaggcacaa
     2221 tccgaggaaa atgcaacaga aacgttatga tgtaacatct gagcacagtc aataaaattg
     2281 catgttgtat gccgcacaca aaagtgttgt taaacatgcg atgtggttgt caaatcatta
     2341 ttcgcacaac catggaccga acctctggca cgctgcatac gctgtattca ttcacatcat
     2401 gttttctact tgtcacatgc tctgcaccaa gtaactccga ttagttaggt tcgcacatgt
     2461 catcggagtg gaccacttcg aatcatttgg ttcgaagata aaccggacta taagctaggt
     2521 agtaactagt gcgactgtct agaatcgcgt atgcgagtgt atgagctcga tatcccctat
     2581 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     2641 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct
     2701 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2761 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2821 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2881 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2941 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     3001 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     3061 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     3121 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     3181 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     3241 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     3301 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     3361 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     3421 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     3481 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     3541 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     3601 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3661 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3721 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3781 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3841 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3901 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3961 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4021 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4081 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4141 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4201 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4261 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4321 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4381 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4441 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4501 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4561 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     4621 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     4681 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     4741 cgttcaccga caaacaacag ataaaacgaa aggcccagac ttccgactga gcctttcgtt
     4801 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     4861 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccacct
//
