LOCUS pGG-UmpRbcS_346-bleGFP-tRbcS 4806 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.6q-cgtp4xy
VERSION urn.local.jonasblomme.6q-cgtp4xy
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
promoter 1..346
/regulatory_class="promoter"
/note="Ulva RbcS promoter"
/modified_by="jonasblomme"
/label="UmpRbcS"
CDS join(381..388,886..1963)
/gene="ble/GFP fusion"
/note="Streptoalloteichus hindustanus ble gene fused with
Aequoria victoria GFP gene (synthetic Ulva codon adapted
construct)"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein/GFP fusion protein"
/protein_id="ABY60800.1"
/db_xref="GI:164523505"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
NEKRDHMVLLEFVTAAGITHGMDELYK"
intron 389..885
/gene="ble/GFP fusion"
/note="derived from Ulva RbcS; enhancer"
/number=1
/label="RbcsI"
Linker 1968..2013
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker"
terminator 2018..2406
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 2411..2459
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(2496..2516)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 2577..2627
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 2628..3437
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 3584..4172
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 4771..4791
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 gtgattgcca gaaacagcaa cccactcact agatataccg ctccaataat gggttcatgt
61 gcaagctcac tcaataacga aacgcttcac tattgtgcac gtgccggggt tgcttgggta
121 tggcgtgcca tacgggttct gcacatgtca cccacgtggg tgtggcacga gatatcgagg
181 tggccatgta aaaggcattt gctagagggc agctcgtcca tgccgctcga gcacaccgag
241 tgcgcaccat aaatgcagca catgcctgtg cagcctatct cctgtctctg ttacatccca
301 ccacctcctc acatatcccc ttccaccctt aaaccgcaca gcagacaaca gtattcagtc
361 gactggtacc aacaggctcc atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg
421 acgtccgctg cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt
481 tgtatcggaa caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc
541 aggtccaatg tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc
601 acgaccccac aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat
661 tctttgacct tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct
721 ggtgctggta ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga
781 ctgtgattga tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata
841 ccctcacgtg agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt
901 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
961 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
1021 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
1081 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
1141 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
1201 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggtac ccaagggcga
1261 ggagctgttc accggtgtgg tccccatcct ggtggagctg gacggcgacg tgaacggcca
1321 caagttctcc gtctccggcg agggtgaggg tgacgccacc tacggcaagc tgaccctgaa
1381 gttcatctgc accaccggca agctgcccgt gccctggccc accctggtca ccaccctgac
1441 ctacggtgtg cagtgcttct cccgctaccc cgaccacatg aagcagcacg acttcttcaa
1501 gtccgccatg cccgagggct acgtgcagga gcgcaccatc ttcttcaagg acgacggcaa
1561 ctacaagacc cgcgccgagg tcaagttcga gggcgacacc ctggtgaacc gcatcgagct
1621 gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacaacta
1681 caactcccac aacgtgtaca tcatggccga caagcagaag aacggcatca aggtgaactt
1741 caagatccgc cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa
1801 cacccccatc ggcgatggcc ccgtgctgct gcccgacaac cactacctgt ccatccagtc
1861 cgccctgtcc aaggacccca acgagaagcg cgaccacatg gtcctgctgg agttcgtcac
1921 cgctgccggc atcacccacg gcatggacga gctgtacaag taatcaggtg gatcctagat
1981 aacctttacc ttcattccct gtaaattggt accctgcgca gtcaggcaga gtcgttgttg
2041 ggtgtatggt tgcacaaaac tagacctcga gctggtcggt tagaaagaga tgcttgtgcg
2101 actggatgta acaggcacaa tccgaggaaa atgcaacaga aacgttatga tgtaacatct
2161 gagcacagtc aataaaattg catgttgtat gccgcacaca aaagtgttgt taaacatgcg
2221 atgtggttgt caaatcatta ttcgcacaac catggaccga acctctggca cgctgcatac
2281 gctgtattca ttcacatcat gttttctact tgtcacatgc tctgcaccaa gtaactccga
2341 ttagttaggt tcgcacatgt catcggagtg gaccacttcg aatcatttgg ttcgaagata
2401 aaccggacta taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt
2461 atgagctcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
2521 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat
2581 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
2641 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
2701 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
2761 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
2821 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
2881 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
2941 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
3001 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
3061 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
3121 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
3181 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
3241 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
3301 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
3361 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
3421 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
3481 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
3541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
3601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
3661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
3721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
3781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
3841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
3901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
3961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
4021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
4081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
4141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
4201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
4261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
4321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
4381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
4441 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
4501 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
4561 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
4621 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagac
4681 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
4741 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
4801 ccacct
//