LOCUS pGG-UmpRbcS_346-bleGFP-tRbcS 4806 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.6q-cgtp4xy VERSION urn.local.jonasblomme.6q-cgtp4xy KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..346 /regulatory_class="promoter" /note="Ulva RbcS promoter" /modified_by="jonasblomme" /label="UmpRbcS" CDS join(381..388,886..1963) /gene="ble/GFP fusion" /note="Streptoalloteichus hindustanus ble gene fused with Aequoria victoria GFP gene (synthetic Ulva codon adapted construct)" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein/GFP fusion protein" /protein_id="ABY60800.1" /db_xref="GI:164523505" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" intron 389..885 /gene="ble/GFP fusion" /note="derived from Ulva RbcS; enhancer" /number=1 /label="RbcsI" Linker 1968..2013 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" terminator 2018..2406 /regulatory_class="terminator" /note="Ulva RbcS terminator" /label="UmtRbcS" Linker 2411..2459 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(2496..2516) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 2577..2627 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 2628..3437 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 3584..4172 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4771..4791 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 gtgattgcca gaaacagcaa cccactcact agatataccg ctccaataat gggttcatgt 61 gcaagctcac tcaataacga aacgcttcac tattgtgcac gtgccggggt tgcttgggta 121 tggcgtgcca tacgggttct gcacatgtca cccacgtggg tgtggcacga gatatcgagg 181 tggccatgta aaaggcattt gctagagggc agctcgtcca tgccgctcga gcacaccgag 241 tgcgcaccat aaatgcagca catgcctgtg cagcctatct cctgtctctg ttacatccca 301 ccacctcctc acatatcccc ttccaccctt aaaccgcaca gcagacaaca gtattcagtc 361 gactggtacc aacaggctcc atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg 421 acgtccgctg cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt 481 tgtatcggaa caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc 541 aggtccaatg tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc 601 acgaccccac aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat 661 tctttgacct tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct 721 ggtgctggta ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga 781 ctgtgattga tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata 841 ccctcacgtg agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt 901 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg 961 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct 1021 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt 1081 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga 1141 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct 1201 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggtac ccaagggcga 1261 ggagctgttc accggtgtgg tccccatcct ggtggagctg gacggcgacg tgaacggcca 1321 caagttctcc gtctccggcg agggtgaggg tgacgccacc tacggcaagc tgaccctgaa 1381 gttcatctgc accaccggca agctgcccgt gccctggccc accctggtca ccaccctgac 1441 ctacggtgtg cagtgcttct cccgctaccc cgaccacatg aagcagcacg acttcttcaa 1501 gtccgccatg cccgagggct acgtgcagga gcgcaccatc ttcttcaagg acgacggcaa 1561 ctacaagacc cgcgccgagg tcaagttcga gggcgacacc ctggtgaacc gcatcgagct 1621 gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacaacta 1681 caactcccac aacgtgtaca tcatggccga caagcagaag aacggcatca aggtgaactt 1741 caagatccgc cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa 1801 cacccccatc ggcgatggcc ccgtgctgct gcccgacaac cactacctgt ccatccagtc 1861 cgccctgtcc aaggacccca acgagaagcg cgaccacatg gtcctgctgg agttcgtcac 1921 cgctgccggc atcacccacg gcatggacga gctgtacaag taatcaggtg gatcctagat 1981 aacctttacc ttcattccct gtaaattggt accctgcgca gtcaggcaga gtcgttgttg 2041 ggtgtatggt tgcacaaaac tagacctcga gctggtcggt tagaaagaga tgcttgtgcg 2101 actggatgta acaggcacaa tccgaggaaa atgcaacaga aacgttatga tgtaacatct 2161 gagcacagtc aataaaattg catgttgtat gccgcacaca aaagtgttgt taaacatgcg 2221 atgtggttgt caaatcatta ttcgcacaac catggaccga acctctggca cgctgcatac 2281 gctgtattca ttcacatcat gttttctact tgtcacatgc tctgcaccaa gtaactccga 2341 ttagttaggt tcgcacatgt catcggagtg gaccacttcg aatcatttgg ttcgaagata 2401 aaccggacta taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt 2461 atgagctcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc 2521 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat 2581 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 2641 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 2701 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc 2761 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 2821 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 2881 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat 2941 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 3001 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 3061 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 3121 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 3181 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 3241 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 3301 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 3361 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 3421 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc 3481 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt 3541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 3781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 3841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 3901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 4381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 4441 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc 4501 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 4561 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 4621 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagac 4681 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc 4741 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc 4801 ccacct //