LOCUS       pGG-UmpRbcS_246-bleGFP-tRbcS        4706 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6p-cgtp4xy
VERSION     urn.local.jonasblomme.6p-cgtp4xy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..246
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /modified_by="jonasblomme"
                     /label="UmpRbcS"
     CDS             join(281..288,786..1863)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          289..785
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          1868..1913
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      1918..2306
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2311..2359
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2396..2416)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2477..2527
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2528..3337
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3484..4072
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4671..4691
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
       61 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      121 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      181 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      241 gcagacaaca gtattcagtc gactggtacc aacaggctcc atggccaagt gcgcatcgcg
      301 tgtcaatgct ggcgctgctg acgtccgctg cctaaactgc atgaaagctc ctgcagtcgc
      361 aagtagtcct tttaacttgt tgtatcggaa caaatggttg tagtctgtgc cgtgtgacga
      421 tcttgagccg cctggtcggc aggtccaatg tcggcctgga ccctgcacac cctgcccacc
      481 ctgccgctgc tcacccgttc acgaccccac aggtgctcgc agcagtaatt gtcgatagat
      541 agcaactgtg ttctgactat tctttgacct tccgcatttg ggactgcggc ggcacaccta
      601 ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct ttccgcgcga gcccacacca
      661 tgcaggcgca tagtaatgga ctgtgattga tttaggcaac tgtatgaaac ctcttcatcg
      721 tgccttggtt gcttgcgata ccctcacgtg agttcatatg accaacgctg gcacgacccc
      781 tgcaggatca ccagcgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
      841 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
      901 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
      961 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     1021 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     1081 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     1141 gagcaggtac ccaagggcga ggagctgttc accggtgtgg tccccatcct ggtggagctg
     1201 gacggcgacg tgaacggcca caagttctcc gtctccggcg agggtgaggg tgacgccacc
     1261 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1321 accctggtca ccaccctgac ctacggtgtg cagtgcttct cccgctaccc cgaccacatg
     1381 aagcagcacg acttcttcaa gtccgccatg cccgagggct acgtgcagga gcgcaccatc
     1441 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tcaagttcga gggcgacacc
     1501 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggc
     1561 cacaagctgg agtacaacta caactcccac aacgtgtaca tcatggccga caagcagaag
     1621 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggctc cgtgcagctg
     1681 gccgaccact accagcagaa cacccccatc ggcgatggcc ccgtgctgct gcccgacaac
     1741 cactacctgt ccatccagtc cgccctgtcc aaggacccca acgagaagcg cgaccacatg
     1801 gtcctgctgg agttcgtcac cgctgccggc atcacccacg gcatggacga gctgtacaag
     1861 taatcaggtg gatcctagat aacctttacc ttcattccct gtaaattggt accctgcgca
     1921 gtcaggcaga gtcgttgttg ggtgtatggt tgcacaaaac tagacctcga gctggtcggt
     1981 tagaaagaga tgcttgtgcg actggatgta acaggcacaa tccgaggaaa atgcaacaga
     2041 aacgttatga tgtaacatct gagcacagtc aataaaattg catgttgtat gccgcacaca
     2101 aaagtgttgt taaacatgcg atgtggttgt caaatcatta ttcgcacaac catggaccga
     2161 acctctggca cgctgcatac gctgtattca ttcacatcat gttttctact tgtcacatgc
     2221 tctgcaccaa gtaactccga ttagttaggt tcgcacatgt catcggagtg gaccacttcg
     2281 aatcatttgg ttcgaagata aaccggacta taagctaggt agtaactagt gcgactgtct
     2341 agaatcgcgt atgcgagtgt atgagctcga tatcccctat agtgagtcgt attacatggt
     2401 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2461 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2521 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2581 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2641 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2701 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2761 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2821 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2881 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2941 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3001 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3061 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3121 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3181 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3241 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3301 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3361 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3421 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3601 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4141 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4201 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4261 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4321 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
     4381 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
     4441 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
     4501 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     4561 ataaaacgaa aggcccagac ttccgactga gcctttcgtt ttatttgatg cctggcagtt
     4621 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
     4681 ggccagtctt aagctcgggc ccacct
//
