LOCUS       pGG-UmpRbcS_146-bleGFP-tRbcS        4606 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6o-cgtp4xy
VERSION     urn.local.jonasblomme.6o-cgtp4xy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..146
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /modified_by="jonasblomme"
                     /label="UmpRbcS"
     CDS             join(181..188,686..1763)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          189..685
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          1768..1813
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      1818..2206
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2211..2259
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2296..2316)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2377..2427
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2428..3237
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3384..3972
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4571..4591
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gctagagggc agctcgtcca tgccgctcga gcacaccgag tgcgcaccat aaatgcagca
       61 catgcctgtg cagcctatct cctgtctctg ttacatccca ccacctcctc acatatcccc
      121 ttccaccctt aaaccgcaca gcagacaaca gtattcagtc gactggtacc aacaggctcc
      181 atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg cctaaactgc
      241 atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa caaatggttg
      301 tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg tcggcctgga
      361 ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac aggtgctcgc
      421 agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct tccgcatttg
      481 ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct
      541 ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga tttaggcaac
      601 tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg agttcatatg
      661 accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc accgcgcgcg
      721 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
      781 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
      841 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
      901 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
      961 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     1021 gcgtgcactt cgtggccgag gagcaggtac ccaagggcga ggagctgttc accggtgtgg
     1081 tccccatcct ggtggagctg gacggcgacg tgaacggcca caagttctcc gtctccggcg
     1141 agggtgaggg tgacgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1201 agctgcccgt gccctggccc accctggtca ccaccctgac ctacggtgtg cagtgcttct
     1261 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgagggct
     1321 acgtgcagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1381 tcaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1441 aggacggcaa catcctgggc cacaagctgg agtacaacta caactcccac aacgtgtaca
     1501 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1561 aggacggctc cgtgcagctg gccgaccact accagcagaa cacccccatc ggcgatggcc
     1621 ccgtgctgct gcccgacaac cactacctgt ccatccagtc cgccctgtcc aaggacccca
     1681 acgagaagcg cgaccacatg gtcctgctgg agttcgtcac cgctgccggc atcacccacg
     1741 gcatggacga gctgtacaag taatcaggtg gatcctagat aacctttacc ttcattccct
     1801 gtaaattggt accctgcgca gtcaggcaga gtcgttgttg ggtgtatggt tgcacaaaac
     1861 tagacctcga gctggtcggt tagaaagaga tgcttgtgcg actggatgta acaggcacaa
     1921 tccgaggaaa atgcaacaga aacgttatga tgtaacatct gagcacagtc aataaaattg
     1981 catgttgtat gccgcacaca aaagtgttgt taaacatgcg atgtggttgt caaatcatta
     2041 ttcgcacaac catggaccga acctctggca cgctgcatac gctgtattca ttcacatcat
     2101 gttttctact tgtcacatgc tctgcaccaa gtaactccga ttagttaggt tcgcacatgt
     2161 catcggagtg gaccacttcg aatcatttgg ttcgaagata aaccggacta taagctaggt
     2221 agtaactagt gcgactgtct agaatcgcgt atgcgagtgt atgagctcga tatcccctat
     2281 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     2341 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct
     2401 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2461 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2521 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2581 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2641 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2701 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2761 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2821 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2881 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2941 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     3001 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     3061 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     3121 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     3181 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     3241 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     3301 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4021 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4081 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4141 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4201 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4261 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     4321 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     4381 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     4441 cgttcaccga caaacaacag ataaaacgaa aggcccagac ttccgactga gcctttcgtt
     4501 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     4561 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccacct
//
