LOCUS       pGG-UmpRbcS_46-bleGFP-tRbcS        4506 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6n-cgtp4xx
VERSION     urn.local.jonasblomme.6n-cgtp4xx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..46
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /modified_by="jonasblomme"
                     /label="UmpRbcS"
     CDS             join(81..88,586..1663)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          89..585
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          1668..1713
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      1718..2106
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /modified_by="jonasblomme"
                     /label="UmtRbcS"
     Linker          2111..2159
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2196..2216)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2277..2327
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2328..3137
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3284..3872
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4471..4491
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccacctcctc acatatcccc ttccaccctt aaaccgcaca gcagacaaca gtattcagtc
       61 gactggtacc aacaggctcc atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg
      121 acgtccgctg cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt
      181 tgtatcggaa caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc
      241 aggtccaatg tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc
      301 acgaccccac aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat
      361 tctttgacct tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct
      421 ggtgctggta ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga
      481 ctgtgattga tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata
      541 ccctcacgtg agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt
      601 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
      661 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
      721 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
      781 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
      841 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
      901 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggtac ccaagggcga
      961 ggagctgttc accggtgtgg tccccatcct ggtggagctg gacggcgacg tgaacggcca
     1021 caagttctcc gtctccggcg agggtgaggg tgacgccacc tacggcaagc tgaccctgaa
     1081 gttcatctgc accaccggca agctgcccgt gccctggccc accctggtca ccaccctgac
     1141 ctacggtgtg cagtgcttct cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1201 gtccgccatg cccgagggct acgtgcagga gcgcaccatc ttcttcaagg acgacggcaa
     1261 ctacaagacc cgcgccgagg tcaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1321 gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacaacta
     1381 caactcccac aacgtgtaca tcatggccga caagcagaag aacggcatca aggtgaactt
     1441 caagatccgc cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa
     1501 cacccccatc ggcgatggcc ccgtgctgct gcccgacaac cactacctgt ccatccagtc
     1561 cgccctgtcc aaggacccca acgagaagcg cgaccacatg gtcctgctgg agttcgtcac
     1621 cgctgccggc atcacccacg gcatggacga gctgtacaag taatcaggtg gatcctagat
     1681 aacctttacc ttcattccct gtaaattggt accctgcgca gtcaggcaga gtcgttgttg
     1741 ggtgtatggt tgcacaaaac tagacctcga gctggtcggt tagaaagaga tgcttgtgcg
     1801 actggatgta acaggcacaa tccgaggaaa atgcaacaga aacgttatga tgtaacatct
     1861 gagcacagtc aataaaattg catgttgtat gccgcacaca aaagtgttgt taaacatgcg
     1921 atgtggttgt caaatcatta ttcgcacaac catggaccga acctctggca cgctgcatac
     1981 gctgtattca ttcacatcat gttttctact tgtcacatgc tctgcaccaa gtaactccga
     2041 ttagttaggt tcgcacatgt catcggagtg gaccacttcg aatcatttgg ttcgaagata
     2101 aaccggacta taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt
     2161 atgagctcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2221 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat
     2281 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2341 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2401 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     2461 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     2521 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     2581 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     2641 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     2701 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     2761 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     2821 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     2881 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2941 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     3001 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     3061 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     3121 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     3181 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     3241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3961 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4021 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4081 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4141 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     4201 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4261 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4321 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagac
     4381 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     4441 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     4501 ccacct
//
