LOCUS       pGG-UM140_0016_1500-bleGFP-tRbcS        5960 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6m-cgtp4xx
VERSION     urn.local.jonasblomme.6m-cgtp4xx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1500
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1535..1542,2040..3117)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          1543..2039
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          3122..3167
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      3172..3560
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          3565..3613
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(3650..3670)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3731..3781
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3782..4591
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4738..5326
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5925..5945
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 atctaggggc caatgtggca gtcaggaatg gctagagatg ctgctagtgt cgtgcatacc
       61 tggtgtactg ccaacagcct cacctgacca tggtgcttgt gtttaaagca agcatgccac
      121 aagtcggcat gtaggcatac ggcaagagct acacagttgg ctgttgacct gtggatgcgt
      181 gaaacagaag tttgaatcgt gccctttcgc gttgatgcac cctgtgtggg agccaggctg
      241 gtgtgtgtgg aagccaggct ggtggcacac ctgtgtgacc tggaggtgga cgaccgggtg
      301 gcgaggcaag tagcagtttc atcaaagaaa cacaagctga tgcatccgcg atagtagcac
      361 gattctaatg tgtgcacgca aacgcatcgg cccaactgga gcaggggatg cgcagcagcg
      421 gccgctggtt ccacgaactc gaagcgcaaa gagcagtccg ctgtgaccag cattccgcta
      481 gtgtcaggga cgatatgaat acgggttggg cacattatgt ttgggtcgag gtcgccatgg
      541 cggacgtgca agcggctgtc gatcacgcct atcggagatg cccagcgcct gcatcgatat
      601 gcaaccagct ctgcaccagc gcagcaatac gacagctgca gccgatggcc tcctagacac
      661 tgtgcaacaa caaaaaacaa ttggaccgtg acggcacagg tgtcgaacac aggggctgag
      721 agaggactgc ctgtgcttgt gctccagtgc tcatacgcca tactgatacg cctccaccga
      781 tggcctcact gcctgcggac actggcagac gctgtcgttt ggatgtcgca tcgccatctt
      841 cagtgatgtt gtggcgttcg gcaggatgta cgcatgagcg attcttctaa acgagcagtg
      901 gtggcacatc agcggctccc tcaaaccaca atgccggctt ggaaggtaga gcatctgctc
      961 ggcaagccga catagacgat acagctagca cagccggctt gcagctcatc aatcagtaca
     1021 cataccgtac gtgtctgaag tatgccttgt tgatatgaac actgtaccgt ggggccagac
     1081 gacacggtct gaagcaccgc ctctgcagca tctcgcacga ttgatgcgca tcaattgctt
     1141 gcaattcgat ttgcatttgg cgcagttgtc tgcacaatct ttgccaacga tcgcatgatc
     1201 cgattcaatg atccgaagca tgatcaatac accgcattag cagtgcatgc gcgattggga
     1261 cacctctggg gggggatctg atgcacgttc gacacgggtg ccgaacctgc gagaaggctc
     1321 actacacgcg tgcggacgca tgcatgcagt gccaaagatc tcgtttgaac tgcgcgccag
     1381 cacattgcag tgcacgcccc gtgccaggcg aaaacgtatt taaccccgaa gccagcatac
     1441 tttctctacc agttcatctc ttgttgatct acctccacca cagtctatca gtgattaaca
     1501 aacagtattc agtcgactgg taccaacagg ctccatggcc aagtgcgcat cgcgtgtcaa
     1561 tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag tcgcaagtag
     1621 tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg acgatcttga
     1681 gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc caccctgccg
     1741 ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat agatagcaac
     1801 tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca cctactatcc
     1861 gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac accatgcagg
     1921 cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc atcgtgcctt
     1981 ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga cccctgcagg
     2041 atcaccagcg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     2101 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     2161 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     2221 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     2281 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     2341 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     2401 gtacccaagg gcgaggagct gttcaccggt gtggtcccca tcctggtgga gctggacggc
     2461 gacgtgaacg gccacaagtt ctccgtctcc ggcgagggtg agggtgacgc cacctacggc
     2521 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg
     2581 gtcaccaccc tgacctacgg tgtgcagtgc ttctcccgct accccgacca catgaagcag
     2641 cacgacttct tcaagtccgc catgcccgag ggctacgtgc aggagcgcac catcttcttc
     2701 aaggacgacg gcaactacaa gacccgcgcc gaggtcaagt tcgagggcga caccctggtg
     2761 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct gggccacaag
     2821 ctggagtaca actacaactc ccacaacgtg tacatcatgg ccgacaagca gaagaacggc
     2881 atcaaggtga acttcaagat ccgccacaac atcgaggacg gctccgtgca gctggccgac
     2941 cactaccagc agaacacccc catcggcgat ggccccgtgc tgctgcccga caaccactac
     3001 ctgtccatcc agtccgccct gtccaaggac cccaacgaga agcgcgacca catggtcctg
     3061 ctggagttcg tcaccgctgc cggcatcacc cacggcatgg acgagctgta caagtaatca
     3121 ggtggatcct agataacctt taccttcatt ccctgtaaat tggtaccctg cgcagtcagg
     3181 cagagtcgtt gttgggtgta tggttgcaca aaactagacc tcgagctggt cggttagaaa
     3241 gagatgcttg tgcgactgga tgtaacaggc acaatccgag gaaaatgcaa cagaaacgtt
     3301 atgatgtaac atctgagcac agtcaataaa attgcatgtt gtatgccgca cacaaaagtg
     3361 ttgttaaaca tgcgatgtgg ttgtcaaatc attattcgca caaccatgga ccgaacctct
     3421 ggcacgctgc atacgctgta ttcattcaca tcatgttttc tacttgtcac atgctctgca
     3481 ccaagtaact ccgattagtt aggttcgcac atgtcatcgg agtggaccac ttcgaatcat
     3541 ttggttcgaa gataaaccgg actataagct aggtagtaac tagtgcgact gtctagaatc
     3601 gcgtatgcga gtgtatgagc tcgatatccc ctatagtgag tcgtattaca tggtcatagc
     3661 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3721 aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     3781 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     3841 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     3901 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     3961 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     4021 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     4081 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     4141 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     4201 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     4261 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     4321 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     4381 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     4441 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     4501 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     4561 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     4621 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     4681 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4741 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4801 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4861 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4921 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4981 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5041 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5101 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5161 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5221 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5281 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5341 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5401 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5461 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5521 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5581 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
     5641 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
     5701 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
     5761 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     5821 cgaaaggccc agacttccga ctgagccttt cgttttattt gatgcctggc agttccctac
     5881 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
     5941 tcttaagctc gggcccacct
//
