LOCUS       pGG-UM140_0016_1000-bleGFP-tRbcS        5460 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6l-cgtp4xx
VERSION     urn.local.jonasblomme.6l-cgtp4xx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1035..1042,1540..2617)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          1043..1539
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          2622..2667
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      2672..3060
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          3065..3113
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(3150..3170)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3231..3281
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3282..4091
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4238..4826
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5425..5445
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 acgggttggg cacattatgt ttgggtcgag gtcgccatgg cggacgtgca agcggctgtc
       61 gatcacgcct atcggagatg cccagcgcct gcatcgatat gcaaccagct ctgcaccagc
      121 gcagcaatac gacagctgca gccgatggcc tcctagacac tgtgcaacaa caaaaaacaa
      181 ttggaccgtg acggcacagg tgtcgaacac aggggctgag agaggactgc ctgtgcttgt
      241 gctccagtgc tcatacgcca tactgatacg cctccaccga tggcctcact gcctgcggac
      301 actggcagac gctgtcgttt ggatgtcgca tcgccatctt cagtgatgtt gtggcgttcg
      361 gcaggatgta cgcatgagcg attcttctaa acgagcagtg gtggcacatc agcggctccc
      421 tcaaaccaca atgccggctt ggaaggtaga gcatctgctc ggcaagccga catagacgat
      481 acagctagca cagccggctt gcagctcatc aatcagtaca cataccgtac gtgtctgaag
      541 tatgccttgt tgatatgaac actgtaccgt ggggccagac gacacggtct gaagcaccgc
      601 ctctgcagca tctcgcacga ttgatgcgca tcaattgctt gcaattcgat ttgcatttgg
      661 cgcagttgtc tgcacaatct ttgccaacga tcgcatgatc cgattcaatg atccgaagca
      721 tgatcaatac accgcattag cagtgcatgc gcgattggga cacctctggg gggggatctg
      781 atgcacgttc gacacgggtg ccgaacctgc gagaaggctc actacacgcg tgcggacgca
      841 tgcatgcagt gccaaagatc tcgtttgaac tgcgcgccag cacattgcag tgcacgcccc
      901 gtgccaggcg aaaacgtatt taaccccgaa gccagcatac tttctctacc agttcatctc
      961 ttgttgatct acctccacca cagtctatca gtgattaaca aacagtattc agtcgactgg
     1021 taccaacagg ctccatggcc aagtgcgcat cgcgtgtcaa tgctggcgct gctgacgtcc
     1081 gctgcctaaa ctgcatgaaa gctcctgcag tcgcaagtag tccttttaac ttgttgtatc
     1141 ggaacaaatg gttgtagtct gtgccgtgtg acgatcttga gccgcctggt cggcaggtcc
     1201 aatgtcggcc tggaccctgc acaccctgcc caccctgccg ctgctcaccc gttcacgacc
     1261 ccacaggtgc tcgcagcagt aattgtcgat agatagcaac tgtgttctga ctattctttg
     1321 accttccgca tttgggactg cggcggcaca cctactatcc gcaatgcaca ccctggtgct
     1381 ggtactgcct gcctttccgc gcgagcccac accatgcagg cgcatagtaa tggactgtga
     1441 ttgatttagg caactgtatg aaacctcttc atcgtgcctt ggttgcttgc gataccctca
     1501 cgtgagttca tatgaccaac gctggcacga cccctgcagg atcaccagcg ccgttccggt
     1561 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     1621 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     1681 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     1741 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     1801 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     1861 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gtacccaagg gcgaggagct
     1921 gttcaccggt gtggtcccca tcctggtgga gctggacggc gacgtgaacg gccacaagtt
     1981 ctccgtctcc ggcgagggtg agggtgacgc cacctacggc aagctgaccc tgaagttcat
     2041 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg gtcaccaccc tgacctacgg
     2101 tgtgcagtgc ttctcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2161 catgcccgag ggctacgtgc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2221 gacccgcgcc gaggtcaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2281 catcgacttc aaggaggacg gcaacatcct gggccacaag ctggagtaca actacaactc
     2341 ccacaacgtg tacatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2401 ccgccacaac atcgaggacg gctccgtgca gctggccgac cactaccagc agaacacccc
     2461 catcggcgat ggccccgtgc tgctgcccga caaccactac ctgtccatcc agtccgccct
     2521 gtccaaggac cccaacgaga agcgcgacca catggtcctg ctggagttcg tcaccgctgc
     2581 cggcatcacc cacggcatgg acgagctgta caagtaatca ggtggatcct agataacctt
     2641 taccttcatt ccctgtaaat tggtaccctg cgcagtcagg cagagtcgtt gttgggtgta
     2701 tggttgcaca aaactagacc tcgagctggt cggttagaaa gagatgcttg tgcgactgga
     2761 tgtaacaggc acaatccgag gaaaatgcaa cagaaacgtt atgatgtaac atctgagcac
     2821 agtcaataaa attgcatgtt gtatgccgca cacaaaagtg ttgttaaaca tgcgatgtgg
     2881 ttgtcaaatc attattcgca caaccatgga ccgaacctct ggcacgctgc atacgctgta
     2941 ttcattcaca tcatgttttc tacttgtcac atgctctgca ccaagtaact ccgattagtt
     3001 aggttcgcac atgtcatcgg agtggaccac ttcgaatcat ttggttcgaa gataaaccgg
     3061 actataagct aggtagtaac tagtgcgact gtctagaatc gcgtatgcga gtgtatgagc
     3121 tcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     3181 ccgtgtctca aaatctctga tgttacattg cacaagataa aataatatca tcatgaacaa
     3241 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     3301 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     3361 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     3421 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     3481 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     3541 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     3601 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     3661 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     3721 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3781 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3841 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3901 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3961 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     4021 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     4081 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     4141 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     4201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4381 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4981 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5101 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     5161 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     5221 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     5281 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agacttccga
     5341 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     5401 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggcccacct
//
