LOCUS       pGG-UM140_0016_250-bleGFP-tRbcS        4710 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6j-cgtp4xx
VERSION     urn.local.jonasblomme.6j-cgtp4xx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..250
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(285..292,790..1867)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          293..789
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          1872..1917
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      1922..2310
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2315..2363
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2400..2420)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2481..2531
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2532..3341
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3488..4076
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4675..4695
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gcgattggga cacctctggg gggggatctg atgcacgttc gacacgggtg ccgaacctgc
       61 gagaaggctc actacacgcg tgcggacgca tgcatgcagt gccaaagatc tcgtttgaac
      121 tgcgcgccag cacattgcag tgcacgcccc gtgccaggcg aaaacgtatt taaccccgaa
      181 gccagcatac tttctctacc agttcatctc ttgttgatct acctccacca cagtctatca
      241 gtgattaaca aacagtattc agtcgactgg taccaacagg ctccatggcc aagtgcgcat
      301 cgcgtgtcaa tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag
      361 tcgcaagtag tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg
      421 acgatcttga gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc
      481 caccctgccg ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat
      541 agatagcaac tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca
      601 cctactatcc gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac
      661 accatgcagg cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc
      721 atcgtgcctt ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga
      781 cccctgcagg atcaccagcg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
      841 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
      901 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
      961 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     1021 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     1081 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     1141 cgaggagcag gtacccaagg gcgaggagct gttcaccggt gtggtcccca tcctggtgga
     1201 gctggacggc gacgtgaacg gccacaagtt ctccgtctcc ggcgagggtg agggtgacgc
     1261 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1321 gcccaccctg gtcaccaccc tgacctacgg tgtgcagtgc ttctcccgct accccgacca
     1381 catgaagcag cacgacttct tcaagtccgc catgcccgag ggctacgtgc aggagcgcac
     1441 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtcaagt tcgagggcga
     1501 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1561 gggccacaag ctggagtaca actacaactc ccacaacgtg tacatcatgg ccgacaagca
     1621 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gctccgtgca
     1681 gctggccgac cactaccagc agaacacccc catcggcgat ggccccgtgc tgctgcccga
     1741 caaccactac ctgtccatcc agtccgccct gtccaaggac cccaacgaga agcgcgacca
     1801 catggtcctg ctggagttcg tcaccgctgc cggcatcacc cacggcatgg acgagctgta
     1861 caagtaatca ggtggatcct agataacctt taccttcatt ccctgtaaat tggtaccctg
     1921 cgcagtcagg cagagtcgtt gttgggtgta tggttgcaca aaactagacc tcgagctggt
     1981 cggttagaaa gagatgcttg tgcgactgga tgtaacaggc acaatccgag gaaaatgcaa
     2041 cagaaacgtt atgatgtaac atctgagcac agtcaataaa attgcatgtt gtatgccgca
     2101 cacaaaagtg ttgttaaaca tgcgatgtgg ttgtcaaatc attattcgca caaccatgga
     2161 ccgaacctct ggcacgctgc atacgctgta ttcattcaca tcatgttttc tacttgtcac
     2221 atgctctgca ccaagtaact ccgattagtt aggttcgcac atgtcatcgg agtggaccac
     2281 ttcgaatcat ttggttcgaa gataaaccgg actataagct aggtagtaac tagtgcgact
     2341 gtctagaatc gcgtatgcga gtgtatgagc tcgatatccc ctatagtgag tcgtattaca
     2401 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2461 cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2521 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2581 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2641 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2701 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2761 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2821 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2881 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2941 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     3001 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     3061 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3121 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3181 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3241 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3301 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3361 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3421 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4201 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4261 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4321 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4381 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4441 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4501 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4561 acagataaaa cgaaaggccc agacttccga ctgagccttt cgttttattt gatgcctggc
     4621 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     4681 cgacggccag tcttaagctc gggcccacct
//
