LOCUS pGG-UM140_0016_125-bleGFP-tRbcS 4585 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.6i-cgtp4xw VERSION urn.local.jonasblomme.6i-cgtp4xw KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..125 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(160..167,665..1742) /gene="ble/GFP fusion" /note="Streptoalloteichus hindustanus ble gene fused with Aequoria victoria GFP gene (synthetic Ulva codon adapted construct)" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein/GFP fusion protein" /protein_id="ABY60800.1" /db_xref="GI:164523505" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" intron 168..664 /gene="ble/GFP fusion" /note="derived from Ulva RbcS; enhancer" /number=1 /label="RbcsI" Linker 1747..1792 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" terminator 1797..2185 /regulatory_class="terminator" /note="Ulva RbcS terminator" /label="UmtRbcS" Linker 2190..2238 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(2275..2295) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 2356..2406 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 2407..3216 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 3363..3951 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4550..4570 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc ccgaagccag 61 catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc tatcagtgat 121 taacaaacag tattcagtcg actggtacca acaggctcca tggccaagtg cgcatcgcgt 181 gtcaatgctg gcgctgctga cgtccgctgc ctaaactgca tgaaagctcc tgcagtcgca 241 agtagtcctt ttaacttgtt gtatcggaac aaatggttgt agtctgtgcc gtgtgacgat 301 cttgagccgc ctggtcggca ggtccaatgt cggcctggac cctgcacacc ctgcccaccc 361 tgccgctgct cacccgttca cgaccccaca ggtgctcgca gcagtaattg tcgatagata 421 gcaactgtgt tctgactatt ctttgacctt ccgcatttgg gactgcggcg gcacacctac 481 tatccgcaat gcacaccctg gtgctggtac tgcctgcctt tccgcgcgag cccacaccat 541 gcaggcgcat agtaatggac tgtgattgat ttaggcaact gtatgaaacc tcttcatcgt 601 gccttggttg cttgcgatac cctcacgtga gttcatatga ccaacgctgg cacgacccct 661 gcaggatcac cagcgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt 721 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg 781 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca 841 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg 901 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt 961 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg 1021 agcaggtacc caagggcgag gagctgttca ccggtgtggt ccccatcctg gtggagctgg 1081 acggcgacgt gaacggccac aagttctccg tctccggcga gggtgagggt gacgccacct 1141 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 1201 ccctggtcac caccctgacc tacggtgtgc agtgcttctc ccgctacccc gaccacatga 1261 agcagcacga cttcttcaag tccgccatgc ccgagggcta cgtgcaggag cgcaccatct 1321 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt caagttcgag ggcgacaccc 1381 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctgggcc 1441 acaagctgga gtacaactac aactcccaca acgtgtacat catggccgac aagcagaaga 1501 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggctcc gtgcagctgg 1561 ccgaccacta ccagcagaac acccccatcg gcgatggccc cgtgctgctg cccgacaacc 1621 actacctgtc catccagtcc gccctgtcca aggaccccaa cgagaagcgc gaccacatgg 1681 tcctgctgga gttcgtcacc gctgccggca tcacccacgg catggacgag ctgtacaagt 1741 aatcaggtgg atcctagata acctttacct tcattccctg taaattggta ccctgcgcag 1801 tcaggcagag tcgttgttgg gtgtatggtt gcacaaaact agacctcgag ctggtcggtt 1861 agaaagagat gcttgtgcga ctggatgtaa caggcacaat ccgaggaaaa tgcaacagaa 1921 acgttatgat gtaacatctg agcacagtca ataaaattgc atgttgtatg ccgcacacaa 1981 aagtgttgtt aaacatgcga tgtggttgtc aaatcattat tcgcacaacc atggaccgaa 2041 cctctggcac gctgcatacg ctgtattcat tcacatcatg ttttctactt gtcacatgct 2101 ctgcaccaag taactccgat tagttaggtt cgcacatgtc atcggagtgg accacttcga 2161 atcatttggt tcgaagataa accggactat aagctaggta gtaactagtg cgactgtcta 2221 gaatcgcgta tgcgagtgta tgagctcgat atcccctata gtgagtcgta ttacatggtc 2281 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa 2341 gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg 2401 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat 2461 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 2521 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 2581 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 2641 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 2701 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 2761 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 2821 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 2881 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 2941 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 3001 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 3061 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 3121 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 3181 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 3241 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 3301 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 3361 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 3421 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 3481 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 3541 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 3601 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 3661 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 3721 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 3781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 3841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 3901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 3961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 4021 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 4081 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 4141 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 4201 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag 4261 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat 4321 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt 4381 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 4441 taaaacgaaa ggcccagact tccgactgag cctttcgttt tatttgatgc ctggcagttc 4501 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg 4561 gccagtctta agctcgggcc cacct //