LOCUS pGG-UM140_0016_125-bleGFP-tRbcS 4585 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.6i-cgtp4xw
VERSION urn.local.jonasblomme.6i-cgtp4xw
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
promoter 1..125
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(160..167,665..1742)
/gene="ble/GFP fusion"
/note="Streptoalloteichus hindustanus ble gene fused with
Aequoria victoria GFP gene (synthetic Ulva codon adapted
construct)"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein/GFP fusion protein"
/protein_id="ABY60800.1"
/db_xref="GI:164523505"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
NEKRDHMVLLEFVTAAGITHGMDELYK"
intron 168..664
/gene="ble/GFP fusion"
/note="derived from Ulva RbcS; enhancer"
/number=1
/label="RbcsI"
Linker 1747..1792
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker"
terminator 1797..2185
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 2190..2238
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(2275..2295)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 2356..2406
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 2407..3216
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 3363..3951
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 4550..4570
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc ccgaagccag
61 catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc tatcagtgat
121 taacaaacag tattcagtcg actggtacca acaggctcca tggccaagtg cgcatcgcgt
181 gtcaatgctg gcgctgctga cgtccgctgc ctaaactgca tgaaagctcc tgcagtcgca
241 agtagtcctt ttaacttgtt gtatcggaac aaatggttgt agtctgtgcc gtgtgacgat
301 cttgagccgc ctggtcggca ggtccaatgt cggcctggac cctgcacacc ctgcccaccc
361 tgccgctgct cacccgttca cgaccccaca ggtgctcgca gcagtaattg tcgatagata
421 gcaactgtgt tctgactatt ctttgacctt ccgcatttgg gactgcggcg gcacacctac
481 tatccgcaat gcacaccctg gtgctggtac tgcctgcctt tccgcgcgag cccacaccat
541 gcaggcgcat agtaatggac tgtgattgat ttaggcaact gtatgaaacc tcttcatcgt
601 gccttggttg cttgcgatac cctcacgtga gttcatatga ccaacgctgg cacgacccct
661 gcaggatcac cagcgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
721 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
781 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
841 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
901 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
961 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
1021 agcaggtacc caagggcgag gagctgttca ccggtgtggt ccccatcctg gtggagctgg
1081 acggcgacgt gaacggccac aagttctccg tctccggcga gggtgagggt gacgccacct
1141 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
1201 ccctggtcac caccctgacc tacggtgtgc agtgcttctc ccgctacccc gaccacatga
1261 agcagcacga cttcttcaag tccgccatgc ccgagggcta cgtgcaggag cgcaccatct
1321 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt caagttcgag ggcgacaccc
1381 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctgggcc
1441 acaagctgga gtacaactac aactcccaca acgtgtacat catggccgac aagcagaaga
1501 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggctcc gtgcagctgg
1561 ccgaccacta ccagcagaac acccccatcg gcgatggccc cgtgctgctg cccgacaacc
1621 actacctgtc catccagtcc gccctgtcca aggaccccaa cgagaagcgc gaccacatgg
1681 tcctgctgga gttcgtcacc gctgccggca tcacccacgg catggacgag ctgtacaagt
1741 aatcaggtgg atcctagata acctttacct tcattccctg taaattggta ccctgcgcag
1801 tcaggcagag tcgttgttgg gtgtatggtt gcacaaaact agacctcgag ctggtcggtt
1861 agaaagagat gcttgtgcga ctggatgtaa caggcacaat ccgaggaaaa tgcaacagaa
1921 acgttatgat gtaacatctg agcacagtca ataaaattgc atgttgtatg ccgcacacaa
1981 aagtgttgtt aaacatgcga tgtggttgtc aaatcattat tcgcacaacc atggaccgaa
2041 cctctggcac gctgcatacg ctgtattcat tcacatcatg ttttctactt gtcacatgct
2101 ctgcaccaag taactccgat tagttaggtt cgcacatgtc atcggagtgg accacttcga
2161 atcatttggt tcgaagataa accggactat aagctaggta gtaactagtg cgactgtcta
2221 gaatcgcgta tgcgagtgta tgagctcgat atcccctata gtgagtcgta ttacatggtc
2281 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
2341 gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
2401 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
2461 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
2521 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
2581 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
2641 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
2701 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
2761 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
2821 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
2881 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
2941 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
3001 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
3061 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
3121 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
3181 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
3241 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
3301 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
3361 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
3421 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
3481 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
3541 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
3601 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
3661 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
3721 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
3781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
3841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
3901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
3961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
4021 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
4081 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
4141 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
4201 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
4261 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
4321 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
4381 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
4441 taaaacgaaa ggcccagact tccgactgag cctttcgttt tatttgatgc ctggcagttc
4501 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
4561 gccagtctta agctcgggcc cacct
//