LOCUS pGG-UM140_0016_63-bleGFP-tRbcS 4523 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.6h-cgtp4xw VERSION urn.local.jonasblomme.6h-cgtp4xw KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..63 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(98..105,603..1680) /gene="ble/GFP fusion" /note="Streptoalloteichus hindustanus ble gene fused with Aequoria victoria GFP gene (synthetic Ulva codon adapted construct)" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein/GFP fusion protein" /protein_id="ABY60800.1" /db_xref="GI:164523505" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" intron 106..602 /gene="ble/GFP fusion" /note="derived from Ulva RbcS; enhancer" /number=1 /label="RbcsI" Linker 1685..1730 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" terminator 1735..2123 /regulatory_class="terminator" /note="Ulva RbcS terminator" /label="UmtRbcS" Linker 2128..2176 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(2213..2233) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 2294..2344 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 2345..3154 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 3301..3889 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4488..4508 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 tactttctct accagttcat ctcttgttga tctacctcca ccacagtcta tcagtgatta 61 acaaacagta ttcagtcgac tggtaccaac aggctccatg gccaagtgcg catcgcgtgt 121 caatgctggc gctgctgacg tccgctgcct aaactgcatg aaagctcctg cagtcgcaag 181 tagtcctttt aacttgttgt atcggaacaa atggttgtag tctgtgccgt gtgacgatct 241 tgagccgcct ggtcggcagg tccaatgtcg gcctggaccc tgcacaccct gcccaccctg 301 ccgctgctca cccgttcacg accccacagg tgctcgcagc agtaattgtc gatagatagc 361 aactgtgttc tgactattct ttgaccttcc gcatttggga ctgcggcggc acacctacta 421 tccgcaatgc acaccctggt gctggtactg cctgcctttc cgcgcgagcc cacaccatgc 481 aggcgcatag taatggactg tgattgattt aggcaactgt atgaaacctc ttcatcgtgc 541 cttggttgct tgcgataccc tcacgtgagt tcatatgacc aacgctggca cgacccctgc 601 aggatcacca gcgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc 661 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc 721 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc 781 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg 841 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg 901 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag 961 caggtaccca agggcgagga gctgttcacc ggtgtggtcc ccatcctggt ggagctggac 1021 ggcgacgtga acggccacaa gttctccgtc tccggcgagg gtgagggtga cgccacctac 1081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 1141 ctggtcacca ccctgaccta cggtgtgcag tgcttctccc gctaccccga ccacatgaag 1201 cagcacgact tcttcaagtc cgccatgccc gagggctacg tgcaggagcg caccatcttc 1261 ttcaaggacg acggcaacta caagacccgc gccgaggtca agttcgaggg cgacaccctg 1321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac 1381 aagctggagt acaactacaa ctcccacaac gtgtacatca tggccgacaa gcagaagaac 1441 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctccgt gcagctggcc 1501 gaccactacc agcagaacac ccccatcggc gatggccccg tgctgctgcc cgacaaccac 1561 tacctgtcca tccagtccgc cctgtccaag gaccccaacg agaagcgcga ccacatggtc 1621 ctgctggagt tcgtcaccgc tgccggcatc acccacggca tggacgagct gtacaagtaa 1681 tcaggtggat cctagataac ctttaccttc attccctgta aattggtacc ctgcgcagtc 1741 aggcagagtc gttgttgggt gtatggttgc acaaaactag acctcgagct ggtcggttag 1801 aaagagatgc ttgtgcgact ggatgtaaca ggcacaatcc gaggaaaatg caacagaaac 1861 gttatgatgt aacatctgag cacagtcaat aaaattgcat gttgtatgcc gcacacaaaa 1921 gtgttgttaa acatgcgatg tggttgtcaa atcattattc gcacaaccat ggaccgaacc 1981 tctggcacgc tgcatacgct gtattcattc acatcatgtt ttctacttgt cacatgctct 2041 gcaccaagta actccgatta gttaggttcg cacatgtcat cggagtggac cacttcgaat 2101 catttggttc gaagataaac cggactataa gctaggtagt aactagtgcg actgtctaga 2161 atcgcgtatg cgagtgtatg agctcgatat cccctatagt gagtcgtatt acatggtcat 2221 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 2281 taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 2341 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc 2401 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 2461 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 2521 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct 2581 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 2641 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 2701 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 2761 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 2821 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 2881 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 2941 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 3001 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 3061 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 3121 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca 3181 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa 3241 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 3481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 3961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 4021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 4081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 4141 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga 4201 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc 4261 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc 4321 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata 4381 aaacgaaagg cccagacttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc 4441 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc 4501 cagtcttaag ctcgggccca cct //