LOCUS pGG-UM140_0016_63-bleGFP-tRbcS 4523 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.6h-cgtp4xw
VERSION urn.local.jonasblomme.6h-cgtp4xw
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
promoter 1..63
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(98..105,603..1680)
/gene="ble/GFP fusion"
/note="Streptoalloteichus hindustanus ble gene fused with
Aequoria victoria GFP gene (synthetic Ulva codon adapted
construct)"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein/GFP fusion protein"
/protein_id="ABY60800.1"
/db_xref="GI:164523505"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
NEKRDHMVLLEFVTAAGITHGMDELYK"
intron 106..602
/gene="ble/GFP fusion"
/note="derived from Ulva RbcS; enhancer"
/number=1
/label="RbcsI"
Linker 1685..1730
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker"
terminator 1735..2123
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 2128..2176
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(2213..2233)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 2294..2344
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 2345..3154
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 3301..3889
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 4488..4508
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 tactttctct accagttcat ctcttgttga tctacctcca ccacagtcta tcagtgatta
61 acaaacagta ttcagtcgac tggtaccaac aggctccatg gccaagtgcg catcgcgtgt
121 caatgctggc gctgctgacg tccgctgcct aaactgcatg aaagctcctg cagtcgcaag
181 tagtcctttt aacttgttgt atcggaacaa atggttgtag tctgtgccgt gtgacgatct
241 tgagccgcct ggtcggcagg tccaatgtcg gcctggaccc tgcacaccct gcccaccctg
301 ccgctgctca cccgttcacg accccacagg tgctcgcagc agtaattgtc gatagatagc
361 aactgtgttc tgactattct ttgaccttcc gcatttggga ctgcggcggc acacctacta
421 tccgcaatgc acaccctggt gctggtactg cctgcctttc cgcgcgagcc cacaccatgc
481 aggcgcatag taatggactg tgattgattt aggcaactgt atgaaacctc ttcatcgtgc
541 cttggttgct tgcgataccc tcacgtgagt tcatatgacc aacgctggca cgacccctgc
601 aggatcacca gcgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
661 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
721 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
781 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
841 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
901 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
961 caggtaccca agggcgagga gctgttcacc ggtgtggtcc ccatcctggt ggagctggac
1021 ggcgacgtga acggccacaa gttctccgtc tccggcgagg gtgagggtga cgccacctac
1081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
1141 ctggtcacca ccctgaccta cggtgtgcag tgcttctccc gctaccccga ccacatgaag
1201 cagcacgact tcttcaagtc cgccatgccc gagggctacg tgcaggagcg caccatcttc
1261 ttcaaggacg acggcaacta caagacccgc gccgaggtca agttcgaggg cgacaccctg
1321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac
1381 aagctggagt acaactacaa ctcccacaac gtgtacatca tggccgacaa gcagaagaac
1441 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctccgt gcagctggcc
1501 gaccactacc agcagaacac ccccatcggc gatggccccg tgctgctgcc cgacaaccac
1561 tacctgtcca tccagtccgc cctgtccaag gaccccaacg agaagcgcga ccacatggtc
1621 ctgctggagt tcgtcaccgc tgccggcatc acccacggca tggacgagct gtacaagtaa
1681 tcaggtggat cctagataac ctttaccttc attccctgta aattggtacc ctgcgcagtc
1741 aggcagagtc gttgttgggt gtatggttgc acaaaactag acctcgagct ggtcggttag
1801 aaagagatgc ttgtgcgact ggatgtaaca ggcacaatcc gaggaaaatg caacagaaac
1861 gttatgatgt aacatctgag cacagtcaat aaaattgcat gttgtatgcc gcacacaaaa
1921 gtgttgttaa acatgcgatg tggttgtcaa atcattattc gcacaaccat ggaccgaacc
1981 tctggcacgc tgcatacgct gtattcattc acatcatgtt ttctacttgt cacatgctct
2041 gcaccaagta actccgatta gttaggttcg cacatgtcat cggagtggac cacttcgaat
2101 catttggttc gaagataaac cggactataa gctaggtagt aactagtgcg actgtctaga
2161 atcgcgtatg cgagtgtatg agctcgatat cccctatagt gagtcgtatt acatggtcat
2221 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
2281 taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
2341 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
2401 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
2461 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
2521 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
2581 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
2641 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
2701 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
2761 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
2821 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
2881 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
2941 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
3001 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
3061 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
3121 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
3181 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
3241 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
3301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
3361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
3421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
3481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
3541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
3601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
3661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
3721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
3781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
3841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
3901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
3961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
4021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
4081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
4141 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
4201 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
4261 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
4321 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
4381 aaacgaaagg cccagacttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
4441 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
4501 cagtcttaag ctcgggccca cct
//