LOCUS       pGG-UM056_0044_500-bleGFP-tRbcS        4960 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6f-cgtp4xw
VERSION     urn.local.jonasblomme.6f-cgtp4xw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..500
                     /modified_by="jonasblomme"
                     /label="pUM056_0044"
     CDS             join(535..542,1040..2117)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          543..1039
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          2122..2167
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      2172..2560
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2565..2613
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2650..2670)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2731..2781
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2782..3591
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3738..4326
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4925..4945
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tcggaggcca aatgattgtt ttttggtgca ttgccatgca tcgttcctct atgttctacc
       61 accttgtgga gcatacatct gtaacgtatc tcatgaattg ggctcaccgg ccgcgcgtgg
      121 ctctgatgca acatatggcg atcgaccaat atcggtggcg cgcggatcgc gcgcgccgag
      181 cgcatgcagg attcattcac ttgattattc ggggatattc gcagtgaaag atccagtggg
      241 gatactcgtt gcccgatcct cacccccaga tacagtcgat cccgcacgca tgcgcacgca
      301 tggcaccgcc agatcgatcg gtgcggatca tgcaggattg tcaggatgca ccccagcagg
      361 atcgcgtgta ggcatggctg cttggtccaa aaaacgacag catgcccgtg tgtctgcctg
      421 agtgcatata tgcagctgcc gtccgcacaa tccttaccag aaccaaactt cgccgcccac
      481 atcgaacagc actagtaaca aacagtattc agtcgactgg taccaacagg ctccatggcc
      541 aagtgcgcat cgcgtgtcaa tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa
      601 gctcctgcag tcgcaagtag tccttttaac ttgttgtatc ggaacaaatg gttgtagtct
      661 gtgccgtgtg acgatcttga gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc
      721 acaccctgcc caccctgccg ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt
      781 aattgtcgat agatagcaac tgtgttctga ctattctttg accttccgca tttgggactg
      841 cggcggcaca cctactatcc gcaatgcaca ccctggtgct ggtactgcct gcctttccgc
      901 gcgagcccac accatgcagg cgcatagtaa tggactgtga ttgatttagg caactgtatg
      961 aaacctcttc atcgtgcctt ggttgcttgc gataccctca cgtgagttca tatgaccaac
     1021 gctggcacga cccctgcagg atcaccagcg ccgttccggt gctcaccgcg cgcgacgtcg
     1081 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     1141 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     1201 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     1261 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     1321 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     1381 acttcgtggc cgaggagcag gtacccaagg gcgaggagct gttcaccggt gtggtcccca
     1441 tcctggtgga gctggacggc gacgtgaacg gccacaagtt ctccgtctcc ggcgagggtg
     1501 agggtgacgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1561 ccgtgccctg gcccaccctg gtcaccaccc tgacctacgg tgtgcagtgc ttctcccgct
     1621 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgag ggctacgtgc
     1681 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtcaagt
     1741 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1801 gcaacatcct gggccacaag ctggagtaca actacaactc ccacaacgtg tacatcatgg
     1861 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1921 gctccgtgca gctggccgac cactaccagc agaacacccc catcggcgat ggccccgtgc
     1981 tgctgcccga caaccactac ctgtccatcc agtccgccct gtccaaggac cccaacgaga
     2041 agcgcgacca catggtcctg ctggagttcg tcaccgctgc cggcatcacc cacggcatgg
     2101 acgagctgta caagtaatca ggtggatcct agataacctt taccttcatt ccctgtaaat
     2161 tggtaccctg cgcagtcagg cagagtcgtt gttgggtgta tggttgcaca aaactagacc
     2221 tcgagctggt cggttagaaa gagatgcttg tgcgactgga tgtaacaggc acaatccgag
     2281 gaaaatgcaa cagaaacgtt atgatgtaac atctgagcac agtcaataaa attgcatgtt
     2341 gtatgccgca cacaaaagtg ttgttaaaca tgcgatgtgg ttgtcaaatc attattcgca
     2401 caaccatgga ccgaacctct ggcacgctgc atacgctgta ttcattcaca tcatgttttc
     2461 tacttgtcac atgctctgca ccaagtaact ccgattagtt aggttcgcac atgtcatcgg
     2521 agtggaccac ttcgaatcat ttggttcgaa gataaaccgg actataagct aggtagtaac
     2581 tagtgcgact gtctagaatc gcgtatgcga gtgtatgagc tcgatatccc ctatagtgag
     2641 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2701 tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata
     2761 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2821 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2881 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2941 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     3001 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     3061 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     3121 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3181 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     3241 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3301 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     3361 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3421 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3481 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3541 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3601 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3661 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3721 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3781 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3841 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3901 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3961 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4021 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4081 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4141 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4201 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4261 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4321 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4381 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4441 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4501 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4561 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     4621 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     4681 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     4741 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     4801 ccgacaaaca acagataaaa cgaaaggccc agacttccga ctgagccttt cgttttattt
     4861 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     4921 cgttgtaaaa cgacggccag tcttaagctc gggcccacct
//
