LOCUS       pGG-UM056_0044_63-bleGFP-tRbcS        4523 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.6c-cgtp4xv
VERSION     urn.local.jonasblomme.6c-cgtp4xv
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..63
                     /modified_by="jonasblomme"
                     /label="pUM056_0044"
     CDS             join(98..105,603..1680)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          106..602
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          1685..1730
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      1735..2123
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2128..2176
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2213..2233)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2294..2344
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2345..3154
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3301..3889
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4488..4508
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gccgtccgca caatccttac cagaaccaaa cttcgccgcc cacatcgaac agcactagta
       61 acaaacagta ttcagtcgac tggtaccaac aggctccatg gccaagtgcg catcgcgtgt
      121 caatgctggc gctgctgacg tccgctgcct aaactgcatg aaagctcctg cagtcgcaag
      181 tagtcctttt aacttgttgt atcggaacaa atggttgtag tctgtgccgt gtgacgatct
      241 tgagccgcct ggtcggcagg tccaatgtcg gcctggaccc tgcacaccct gcccaccctg
      301 ccgctgctca cccgttcacg accccacagg tgctcgcagc agtaattgtc gatagatagc
      361 aactgtgttc tgactattct ttgaccttcc gcatttggga ctgcggcggc acacctacta
      421 tccgcaatgc acaccctggt gctggtactg cctgcctttc cgcgcgagcc cacaccatgc
      481 aggcgcatag taatggactg tgattgattt aggcaactgt atgaaacctc ttcatcgtgc
      541 cttggttgct tgcgataccc tcacgtgagt tcatatgacc aacgctggca cgacccctgc
      601 aggatcacca gcgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
      661 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
      721 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
      781 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
      841 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
      901 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
      961 caggtaccca agggcgagga gctgttcacc ggtgtggtcc ccatcctggt ggagctggac
     1021 ggcgacgtga acggccacaa gttctccgtc tccggcgagg gtgagggtga cgccacctac
     1081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1141 ctggtcacca ccctgaccta cggtgtgcag tgcttctccc gctaccccga ccacatgaag
     1201 cagcacgact tcttcaagtc cgccatgccc gagggctacg tgcaggagcg caccatcttc
     1261 ttcaaggacg acggcaacta caagacccgc gccgaggtca agttcgaggg cgacaccctg
     1321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac
     1381 aagctggagt acaactacaa ctcccacaac gtgtacatca tggccgacaa gcagaagaac
     1441 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctccgt gcagctggcc
     1501 gaccactacc agcagaacac ccccatcggc gatggccccg tgctgctgcc cgacaaccac
     1561 tacctgtcca tccagtccgc cctgtccaag gaccccaacg agaagcgcga ccacatggtc
     1621 ctgctggagt tcgtcaccgc tgccggcatc acccacggca tggacgagct gtacaagtaa
     1681 tcaggtggat cctagataac ctttaccttc attccctgta aattggtacc ctgcgcagtc
     1741 aggcagagtc gttgttgggt gtatggttgc acaaaactag acctcgagct ggtcggttag
     1801 aaagagatgc ttgtgcgact ggatgtaaca ggcacaatcc gaggaaaatg caacagaaac
     1861 gttatgatgt aacatctgag cacagtcaat aaaattgcat gttgtatgcc gcacacaaaa
     1921 gtgttgttaa acatgcgatg tggttgtcaa atcattattc gcacaaccat ggaccgaacc
     1981 tctggcacgc tgcatacgct gtattcattc acatcatgtt ttctacttgt cacatgctct
     2041 gcaccaagta actccgatta gttaggttcg cacatgtcat cggagtggac cacttcgaat
     2101 catttggttc gaagataaac cggactataa gctaggtagt aactagtgcg actgtctaga
     2161 atcgcgtatg cgagtgtatg agctcgatat cccctatagt gagtcgtatt acatggtcat
     2221 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2281 taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2341 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     2401 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2461 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2521 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2581 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2641 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2701 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2761 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2821 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2881 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2941 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3001 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3061 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3121 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3181 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3241 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4141 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
     4201 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
     4261 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     4321 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     4381 aaacgaaagg cccagacttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     4441 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     4501 cagtcttaag ctcgggccca cct
//
