LOCUS       pGG-pUBI-bleGFP-tRbcS        5093 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.gh-cgtpa2w
VERSION     urn.local.jonasblomme.gh-cgtpa2w
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..633
                     /label="pUBI"
     CDS             join(668..675,1173..2250)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          676..1172
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          2255..2300
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      2305..2693
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2698..2746
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2783..2803)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2864..2914
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2915..3724
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3871..4459
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5058..5078
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
       61 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
      121 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
      181 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
      241 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
      301 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
      361 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
      421 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
      481 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
      541 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
      601 tttgtagatt aatctgagtt tttctgatta acaaacagta ttcagtcgac tggtaccaac
      661 aggctccatg gccaagtgcg catcgcgtgt caatgctggc gctgctgacg tccgctgcct
      721 aaactgcatg aaagctcctg cagtcgcaag tagtcctttt aacttgttgt atcggaacaa
      781 atggttgtag tctgtgccgt gtgacgatct tgagccgcct ggtcggcagg tccaatgtcg
      841 gcctggaccc tgcacaccct gcccaccctg ccgctgctca cccgttcacg accccacagg
      901 tgctcgcagc agtaattgtc gatagatagc aactgtgttc tgactattct ttgaccttcc
      961 gcatttggga ctgcggcggc acacctacta tccgcaatgc acaccctggt gctggtactg
     1021 cctgcctttc cgcgcgagcc cacaccatgc aggcgcatag taatggactg tgattgattt
     1081 aggcaactgt atgaaacctc ttcatcgtgc cttggttgct tgcgataccc tcacgtgagt
     1141 tcatatgacc aacgctggca cgacccctgc aggatcacca gcgccgttcc ggtgctcacc
     1201 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     1261 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     1321 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     1381 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     1441 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     1501 ggcaactgcg tgcacttcgt ggccgaggag caggtaccca agggcgagga gctgttcacc
     1561 ggtgtggtcc ccatcctggt ggagctggac ggcgacgtga acggccacaa gttctccgtc
     1621 tccggcgagg gtgagggtga cgccacctac ggcaagctga ccctgaagtt catctgcacc
     1681 accggcaagc tgcccgtgcc ctggcccacc ctggtcacca ccctgaccta cggtgtgcag
     1741 tgcttctccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1801 gagggctacg tgcaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1861 gccgaggtca agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1921 ttcaaggagg acggcaacat cctgggccac aagctggagt acaactacaa ctcccacaac
     1981 gtgtacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2041 aacatcgagg acggctccgt gcagctggcc gaccactacc agcagaacac ccccatcggc
     2101 gatggccccg tgctgctgcc cgacaaccac tacctgtcca tccagtccgc cctgtccaag
     2161 gaccccaacg agaagcgcga ccacatggtc ctgctggagt tcgtcaccgc tgccggcatc
     2221 acccacggca tggacgagct gtacaagtaa tcaggtggat cctagataac ctttaccttc
     2281 attccctgta aattggtacc ctgcgcagtc aggcagagtc gttgttgggt gtatggttgc
     2341 acaaaactag acctcgagct ggtcggttag aaagagatgc ttgtgcgact ggatgtaaca
     2401 ggcacaatcc gaggaaaatg caacagaaac gttatgatgt aacatctgag cacagtcaat
     2461 aaaattgcat gttgtatgcc gcacacaaaa gtgttgttaa acatgcgatg tggttgtcaa
     2521 atcattattc gcacaaccat ggaccgaacc tctggcacgc tgcatacgct gtattcattc
     2581 acatcatgtt ttctacttgt cacatgctct gcaccaagta actccgatta gttaggttcg
     2641 cacatgtcat cggagtggac cacttcgaat catttggttc gaagataaac cggactataa
     2701 gctaggtagt aactagtgcg actgtctaga atcgcgtatg cgagtgtatg agctcgatat
     2761 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2821 tcaaaatctc tgatgttaca ttgcacaaga taaaataata tcatcatgaa caataaaact
     2881 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2941 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     3001 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     3061 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     3121 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     3181 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     3241 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     3301 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     3361 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     3421 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     3481 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     3541 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     3601 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     3661 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     3721 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     3781 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3841 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3901 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3961 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4021 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4081 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4141 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4201 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4261 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4321 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4381 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4441 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4501 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4561 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4621 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4681 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4741 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     4801 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     4861 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     4921 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagacttc cgactgagcc
     4981 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     5041 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccca cct
//
