LOCUS       pGG-pUBI-bleGFP-NOST        4972 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.ga-cgtpa2v
VERSION     urn.local.jonasblomme.ga-cgtpa2v
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..633
                     /label="pUBI"
     CDS             join(668..675,1173..2250)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          676..1172
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          2255..2300
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      2305..2572
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          2577..2625
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2662..2682)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2743..2793
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2794..3603
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3750..4338
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4937..4957
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
       61 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
      121 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
      181 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
      241 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
      301 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
      361 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
      421 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
      481 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
      541 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
      601 tttgtagatt aatctgagtt tttctgatta acaaacagta ttcagtcgac tggtaccaac
      661 aggctccatg gccaagtgcg catcgcgtgt caatgctggc gctgctgacg tccgctgcct
      721 aaactgcatg aaagctcctg cagtcgcaag tagtcctttt aacttgttgt atcggaacaa
      781 atggttgtag tctgtgccgt gtgacgatct tgagccgcct ggtcggcagg tccaatgtcg
      841 gcctggaccc tgcacaccct gcccaccctg ccgctgctca cccgttcacg accccacagg
      901 tgctcgcagc agtaattgtc gatagatagc aactgtgttc tgactattct ttgaccttcc
      961 gcatttggga ctgcggcggc acacctacta tccgcaatgc acaccctggt gctggtactg
     1021 cctgcctttc cgcgcgagcc cacaccatgc aggcgcatag taatggactg tgattgattt
     1081 aggcaactgt atgaaacctc ttcatcgtgc cttggttgct tgcgataccc tcacgtgagt
     1141 tcatatgacc aacgctggca cgacccctgc aggatcacca gcgccgttcc ggtgctcacc
     1201 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     1261 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     1321 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     1381 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     1441 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     1501 ggcaactgcg tgcacttcgt ggccgaggag caggtaccca agggcgagga gctgttcacc
     1561 ggtgtggtcc ccatcctggt ggagctggac ggcgacgtga acggccacaa gttctccgtc
     1621 tccggcgagg gtgagggtga cgccacctac ggcaagctga ccctgaagtt catctgcacc
     1681 accggcaagc tgcccgtgcc ctggcccacc ctggtcacca ccctgaccta cggtgtgcag
     1741 tgcttctccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1801 gagggctacg tgcaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1861 gccgaggtca agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1921 ttcaaggagg acggcaacat cctgggccac aagctggagt acaactacaa ctcccacaac
     1981 gtgtacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2041 aacatcgagg acggctccgt gcagctggcc gaccactacc agcagaacac ccccatcggc
     2101 gatggccccg tgctgctgcc cgacaaccac tacctgtcca tccagtccgc cctgtccaag
     2161 gaccccaacg agaagcgcga ccacatggtc ctgctggagt tcgtcaccgc tgccggcatc
     2221 acccacggca tggacgagct gtacaagtaa tcaggtggat cctagataac ctttaccttc
     2281 attccctgta aattggtacc ctgcctagag tcaagcagat cgttcaaaca tttggcaata
     2341 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     2401 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     2461 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     2521 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gaactataag
     2581 ctaggtagta actagtgcga ctgtctagaa tcgcgtatgc gagtgtatga gctcgatatc
     2641 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     2701 caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac aataaaactg
     2761 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     2821 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2881 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2941 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     3001 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     3061 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     3121 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     3181 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     3241 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     3301 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     3361 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     3421 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     3481 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3541 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3601 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3661 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3721 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3781 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3841 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3901 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3961 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4021 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4081 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4141 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4201 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4261 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4321 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4381 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4441 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4501 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4561 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4621 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     4681 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     4741 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     4801 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagacttcc gactgagcct
     4861 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     4921 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccac ct
//
