LOCUS       pGG-p35S-bleGFP-NOST        5203 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.g8-cgtpa2v
VERSION     urn.local.jonasblomme.g8-cgtpa2v
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1n-c4nnd54"">pCaMV 35S</a>"
                     /modified_by="mamee"
                     /label="p35S"
     CDS             join(899..906,1404..2481)
                     /gene="ble/GFP fusion"
                     /note="Streptoalloteichus hindustanus ble gene fused with
                     Aequoria victoria GFP gene (synthetic Ulva codon adapted
                     construct)"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein/GFP fusion protein"
                     /protein_id="ABY60800.1"
                     /db_xref="GI:164523505"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
                     ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP
                     NEKRDHMVLLEFVTAAGITHGMDELYK"
     intron          907..1403
                     /gene="ble/GFP fusion"
                     /note="derived from Ulva RbcS; enhancer"
                     /number=1
                     /label="RbcsI"
     Linker          2486..2531
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
     terminator      2536..2803
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          2808..2856
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2893..2913)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2974..3024
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3025..3834
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3981..4569
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5168..5188
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac gtccgctgcc taaactgcat
      961 gaaagctcct gcagtcgcaa gtagtccttt taacttgttg tatcggaaca aatggttgta
     1021 gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag gtccaatgtc ggcctggacc
     1081 ctgcacaccc tgcccaccct gccgctgctc acccgttcac gaccccacag gtgctcgcag
     1141 cagtaattgt cgatagatag caactgtgtt ctgactattc tttgaccttc cgcatttggg
     1201 actgcggcgg cacacctact atccgcaatg cacaccctgg tgctggtact gcctgccttt
     1261 ccgcgcgagc ccacaccatg caggcgcata gtaatggact gtgattgatt taggcaactg
     1321 tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc ctcacgtgag ttcatatgac
     1381 caacgctggc acgacccctg caggatcacc agcgccgttc cggtgctcac cgcgcgcgac
     1441 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     1501 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     1561 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     1621 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     1681 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     1741 gtgcacttcg tggccgagga gcaggtaccc aagggcgagg agctgttcac cggtgtggtc
     1801 cccatcctgg tggagctgga cggcgacgtg aacggccaca agttctccgt ctccggcgag
     1861 ggtgagggtg acgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1921 ctgcccgtgc cctggcccac cctggtcacc accctgacct acggtgtgca gtgcttctcc
     1981 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgagggctac
     2041 gtgcaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtc
     2101 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2161 gacggcaaca tcctgggcca caagctggag tacaactaca actcccacaa cgtgtacatc
     2221 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2281 gacggctccg tgcagctggc cgaccactac cagcagaaca cccccatcgg cgatggcccc
     2341 gtgctgctgc ccgacaacca ctacctgtcc atccagtccg ccctgtccaa ggaccccaac
     2401 gagaagcgcg accacatggt cctgctggag ttcgtcaccg ctgccggcat cacccacggc
     2461 atggacgagc tgtacaagta atcaggtgga tcctagataa cctttacctt cattccctgt
     2521 aaattggtac cctgcctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt
     2581 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     2641 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     2701 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     2761 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgaactataa gctaggtagt
     2821 aactagtgcg actgtctaga atcgcgtatg cgagtgtatg agctcgatat cccctatagt
     2881 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2941 tgatgttaca ttgcacaaga taaaataata tcatcatgaa caataaaact gtctgcttac
     3001 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     3061 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     3121 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     3181 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     3241 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3301 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     3361 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3421 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3481 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3541 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3601 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3661 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3721 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3781 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3841 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3901 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4621 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4681 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4741 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4801 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4861 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4921 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4981 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     5041 tcaccgacaa acaacagata aaacgaaagg cccagacttc cgactgagcc tttcgtttta
     5101 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     5161 cgacgttgta aaacgacggc cagtcttaag ctcgggccca cct
//
