LOCUS       pGG-pUM140_0016+Intron-BleR-tRbcS        5629 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.hv-cgtpf03
VERSION     urn.local.jonasblomme.hv-cgtpf03
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..2431
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     intron          1791..2287
                     /label="RbcsI"
     CDS             2466..2840
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /modified_by="mamee"
     terminator      2841..3229
                     /regulatory_class="terminator"
                     /note="Ulva mutabilis RbcS terminator"
                     /label="UmtRbcS"
     Linker          3234..3282
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(3319..3339)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3400..3450
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3451..4260
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4407..4995
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5594..5614
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
       61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
      121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
      181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
      241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
      301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
      361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
      421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
      481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
      541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
      601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
      661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
      721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
      781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
      841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
      901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
      961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
     1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
     1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
     1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
     1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
     1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
     1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
     1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
     1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
     1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
     1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
     1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
     1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
     1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gtgcgcatcg
     1801 cgtgtcaatg ctggcgctgc tgacgtccgc tgcctaaact gcatgaaagc tcctgcagtc
     1861 gcaagtagtc cttttaactt gttgtatcgg aacaaatggt tgtagtctgt gccgtgtgac
     1921 gatcttgagc cgcctggtcg gcaggtccaa tgtcggcctg gaccctgcac accctgccca
     1981 ccctgccgct gctcacccgt tcacgacccc acaggtgctc gcagcagtaa ttgtcgatag
     2041 atagcaactg tgttctgact attctttgac cttccgcatt tgggactgcg gcggcacacc
     2101 tactatccgc aatgcacacc ctggtgctgg tactgcctgc ctttccgcgc gagcccacac
     2161 catgcaggcg catagtaatg gactgtgatt gatttaggca actgtatgaa acctcttcat
     2221 cgtgccttgg ttgcttgcga taccctcacg tgagttcata tgaccaacgc tggcacgacc
     2281 cctgcaggat ctcgtttgaa ctgcgcgcca gcacattgca gtgcacgccc cgtgccaggc
     2341 gaaaacgtat ttaaccccga agccagcata ctttctctac cagttcatct cttgttgatc
     2401 tacctccacc acagtctatc agtgattaac aaacagtatt cagtcgactg gtaccaacag
     2461 gctccatggc caagatcacc agcgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     2521 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     2581 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     2641 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     2701 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     2761 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     2821 tggccgagga gcaggactaa gcagtcaggc agagtcgttg ttgggtgtat ggttgcacaa
     2881 agctagacct cgagctggtc ggttagaaag agatgcttgt gcgactggat gtaacaggca
     2941 caatccgagg aaaatgcaac agaaacgtta tgatgtaaca tctgagcaca gtcaataaaa
     3001 ttgcatgttg tatgccgcac acaaaagtgt tgttaaacat gcgatgtggt tgtcaaatca
     3061 ttattcgcac aaccatggac cgaacctctg gcacgctgca tacgctgtat tcattcacat
     3121 catgttttct acttgtcaca tgctctgcac caagtaactc cgattagtta ggttcgcaca
     3181 tgtcatcgga gtggaccact tcgaatcatt tggttcgaag ataaaccggt cagtaagcta
     3241 ggtagtaact agtgcgactg tctagaatcg cgtatgcgag tgtatgagct cgatatcccc
     3301 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     3361 aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct
     3421 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     3481 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3541 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3601 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3661 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3721 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3781 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3841 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3901 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3961 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     4021 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     4081 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     4141 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     4201 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     4261 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     4321 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     4381 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4441 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4501 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4561 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4621 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4681 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4741 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4801 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4861 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4921 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4981 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5041 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5101 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5161 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5221 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5281 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     5341 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     5401 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     5461 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gacttccgac tgagcctttc
     5521 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     5581 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccacct
//
