LOCUS       pGG-pUM056_0044-RubiscoIntron-BleR-tRbcS        4975 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.hu-cgtpf03
VERSION     urn.local.jonasblomme.hu-cgtpf03
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..1304
                     /modified_by="jonasblomme"
                     /label="pUM056_0044"
     intron          1305..1805
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     CDS             1812..2186
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /modified_by="mamee"
     terminator      2187..2575
                     /regulatory_class="terminator"
                     /note="Ulva mutabilis RbcS terminator"
                     /label="UmtRbcS"
     Linker          2580..2628
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2665..2685)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2746..2796
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2797..3606
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3753..4341
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4940..4960
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cggaagagtc ggaacgacat taatcaatag ctgcagttga tggctggggt accagcagct
       61 gcagttgatg gctggggtgc accgcagctc caggtcgagg tccggtaccg gtgtcggggg
      121 ctgtcgcggt tgtgggcatg ccccgtgaag ccagtaagta tggcttccgt cccgcaagag
      181 cgagcatgtc caccgaaaat tgcttcacgt ggcatgaccc atagcgagtg cgccgtagct
      241 ccagagtggt gctctaagta accagtcgtg gggtcagcag gacatgtaca tatgtccgga
      301 cacagcacgc gcaggattgg aaacatcacc ccacgccaat ttcatctaca cagttctgat
      361 gtggtaaact gtcacgactg ctgcagcgtc gctcgatctc atgatcactg ccccttgacg
      421 cagatgagag tcacgatgac aatctgtacc agtgataagt ccaaaattag caagtaggta
      481 ctgcgtggtg tccgtttggt cgtgttcgca ttcctgcccg tgcatgcgcc cgcatcaatt
      541 tcgcccgcaa cgtacagagt ggtcactctg acagctatag gggggaaatg gtcacccggg
      601 tggccatgcg ggcaaaagtc gttcatgagt gttgcgacca ctgcccgagc gctgggcacg
      661 tgattggtat ttcacttgag ctgacattcc gcaacattcc attgcgctag agacatgcag
      721 gtttacgcgt gccagtggca tggcatgggg aagtcgagct gtgcgcagct gcaggggctg
      781 gattcgcgtt gggcccaatt gggctcggag gccaaatgat tgttttttgg tgcattgcca
      841 tgcatcgttc ctctatgttc taccaccttg tggagcatac atctgtaacg tatctcatga
      901 attgggctca ccggccgcgc gtggctctga tgcaacatat ggcgatcgac caatatcggt
      961 ggcgcgcgga tcgcgcgcgc cgagcgcatg caggattcat tcacttgatt attcggggat
     1021 attcgcagtg aaagatccag tggggatact cgttgcccga tcctcacccc cagatacagt
     1081 cgatcccgca cgcatgcgca cgcatggcac cgccagatcg atcggtgcgg atcatgcagg
     1141 attgtcagga tgcaccccag caggatcgcg tgtaggcatg gctgcttggt ccaaaaaacg
     1201 acagcatgcc cgtgtgtctg cctgagtgca tatatgcagc tgccgtccgc acaatcctta
     1261 ccagaaccaa acttcgccgc ccacatcgaa cagcactagt aacaaacagt gcgcatcgcg
     1321 tgtcaatgct ggcgctgctg acgtccgctg cctaaactgc atgaaagctc ctgcagtcgc
     1381 aagtagtcct tttaacttgt tgtatcggaa caaatggttg tagtctgtgc cgtgtgacga
     1441 tcttgagccg cctggtcggc aggtccaatg tcggcctgga ccctgcacac cctgcccacc
     1501 ctgccgctgc tcacccgttc acgaccccac aggtgctcgc agcagtaatt gtcgatagat
     1561 agcaactgtg ttctgactat tctttgacct tccgcatttg ggactgcggc ggcacaccta
     1621 ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct ttccgcgcga gcccacacca
     1681 tgcaggcgca tagtaatgga ctgtgattga tttaggcaac tgtatgaaac ctcttcatcg
     1741 tgccttggtt gcttgcgata ccctcacgtg agttcatatg accaacgctg gcacgacccc
     1801 tgcagggctc catggccaag atcaccagcg ccgttccggt gctcaccgcg cgcgacgtcg
     1861 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     1921 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     1981 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     2041 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     2101 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     2161 acttcgtggc cgaggagcag gactaagcag tcaggcagag tcgttgttgg gtgtatggtt
     2221 gcacaaagct agacctcgag ctggtcggtt agaaagagat gcttgtgcga ctggatgtaa
     2281 caggcacaat ccgaggaaaa tgcaacagaa acgttatgat gtaacatctg agcacagtca
     2341 ataaaattgc atgttgtatg ccgcacacaa aagtgttgtt aaacatgcga tgtggttgtc
     2401 aaatcattat tcgcacaacc atggaccgaa cctctggcac gctgcatacg ctgtattcat
     2461 tcacatcatg ttttctactt gtcacatgct ctgcaccaag taactccgat tagttaggtt
     2521 cgcacatgtc atcggagtgg accacttcga atcatttggt tcgaagataa accggtcagt
     2581 aagctaggta gtaactagtg cgactgtcta gaatcgcgta tgcgagtgta tgagctcgat
     2641 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2701 tctcaaaatc tctgatgtta cattgcacaa gataaaataa tatcatcatg aacaataaaa
     2761 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2821 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2881 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2941 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3001 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     3061 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     3121 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     3181 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     3241 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3301 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     3361 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3421 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3481 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3541 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3601 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3661 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4321 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4381 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4441 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4501 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4561 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4621 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     4681 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     4741 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     4801 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagact tccgactgag
     4861 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4921 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc cacct
//
