LOCUS       pGG-pUM056_0044-GS-RbcSIntron-BleR-tRbcS        4993 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.ht-cgtpf03
VERSION     urn.local.jonasblomme.ht-cgtpf03
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..1304
                     /modified_by="jonasblomme"
                     /label="pUM056_0044"
     CDS             join(1309..1316,1814..1823)
                     /gene="GS LINKER"
                     /codon_start=1
     intron          1317..1813
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /label="RbcsI"
     CDS             1830..2204
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /modified_by="mamee"
     terminator      2205..2593
                     /regulatory_class="terminator"
                     /note="Ulva mutabilis RbcS terminator"
                     /label="UmtRbcS"
     Linker          2598..2646
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2683..2703)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2764..2814
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2815..3624
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3771..4359
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4958..4978
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cggaagagtc ggaacgacat taatcaatag ctgcagttga tggctggggt accagcagct
       61 gcagttgatg gctggggtgc accgcagctc caggtcgagg tccggtaccg gtgtcggggg
      121 ctgtcgcggt tgtgggcatg ccccgtgaag ccagtaagta tggcttccgt cccgcaagag
      181 cgagcatgtc caccgaaaat tgcttcacgt ggcatgaccc atagcgagtg cgccgtagct
      241 ccagagtggt gctctaagta accagtcgtg gggtcagcag gacatgtaca tatgtccgga
      301 cacagcacgc gcaggattgg aaacatcacc ccacgccaat ttcatctaca cagttctgat
      361 gtggtaaact gtcacgactg ctgcagcgtc gctcgatctc atgatcactg ccccttgacg
      421 cagatgagag tcacgatgac aatctgtacc agtgataagt ccaaaattag caagtaggta
      481 ctgcgtggtg tccgtttggt cgtgttcgca ttcctgcccg tgcatgcgcc cgcatcaatt
      541 tcgcccgcaa cgtacagagt ggtcactctg acagctatag gggggaaatg gtcacccggg
      601 tggccatgcg ggcaaaagtc gttcatgagt gttgcgacca ctgcccgagc gctgggcacg
      661 tgattggtat ttcacttgag ctgacattcc gcaacattcc attgcgctag agacatgcag
      721 gtttacgcgt gccagtggca tggcatgggg aagtcgagct gtgcgcagct gcaggggctg
      781 gattcgcgtt gggcccaatt gggctcggag gccaaatgat tgttttttgg tgcattgcca
      841 tgcatcgttc ctctatgttc taccaccttg tggagcatac atctgtaacg tatctcatga
      901 attgggctca ccggccgcgc gtggctctga tgcaacatat ggcgatcgac caatatcggt
      961 ggcgcgcgga tcgcgcgcgc cgagcgcatg caggattcat tcacttgatt attcggggat
     1021 attcgcagtg aaagatccag tggggatact cgttgcccga tcctcacccc cagatacagt
     1081 cgatcccgca cgcatgcgca cgcatggcac cgccagatcg atcggtgcgg atcatgcagg
     1141 attgtcagga tgcaccccag caggatcgcg tgtaggcatg gctgcttggt ccaaaaaacg
     1201 acagcatgcc cgtgtgtctg cctgagtgca tatatgcagc tgccgtccgc acaatcctta
     1261 ccagaaccaa acttcgccgc ccacatcgaa cagcactagt aacaaacaat gggcgggtgc
     1321 gcatcgcgtg tcaatgctgg cgctgctgac gtccgctgcc taaactgcat gaaagctcct
     1381 gcagtcgcaa gtagtccttt taacttgttg tatcggaaca aatggttgta gtctgtgccg
     1441 tgtgacgatc ttgagccgcc tggtcggcag gtccaatgtc ggcctggacc ctgcacaccc
     1501 tgcccaccct gccgctgctc acccgttcac gaccccacag gtgctcgcag cagtaattgt
     1561 cgatagatag caactgtgtt ctgactattc tttgaccttc cgcatttggg actgcggcgg
     1621 cacacctact atccgcaatg cacaccctgg tgctggtact gcctgccttt ccgcgcgagc
     1681 ccacaccatg caggcgcata gtaatggact gtgattgatt taggcaactg tatgaaacct
     1741 cttcatcgtg ccttggttgc ttgcgatacc ctcacgtgag ttcatatgac caacgctggc
     1801 acgacccctg caggggcggc agcggctcca tggccaagat caccagcgcc gttccggtgc
     1861 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     1921 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     1981 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     2041 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     2101 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     2161 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctaagcagtc aggcagagtc
     2221 gttgttgggt gtatggttgc acaaagctag acctcgagct ggtcggttag aaagagatgc
     2281 ttgtgcgact ggatgtaaca ggcacaatcc gaggaaaatg caacagaaac gttatgatgt
     2341 aacatctgag cacagtcaat aaaattgcat gttgtatgcc gcacacaaaa gtgttgttaa
     2401 acatgcgatg tggttgtcaa atcattattc gcacaaccat ggaccgaacc tctggcacgc
     2461 tgcatacgct gtattcattc acatcatgtt ttctacttgt cacatgctct gcaccaagta
     2521 actccgatta gttaggttcg cacatgtcat cggagtggac cacttcgaat catttggttc
     2581 gaagataaac cggtcagtaa gctaggtagt aactagtgcg actgtctaga atcgcgtatg
     2641 cgagtgtatg agctcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2701 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaataata
     2761 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2821 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2881 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2941 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     3001 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     3061 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     3121 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     3181 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     3241 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3301 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     3361 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3421 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3481 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3541 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3601 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3661 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3721 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3781 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3841 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3901 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3961 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4021 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4081 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4141 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4201 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4261 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4321 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4381 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4441 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4501 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4561 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4621 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     4681 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4741 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4801 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4861 cccagacttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4921 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4981 ctcgggccca cct
//
