LOCUS       pGG-pUM056_0044+Intron-BleR-tRbcS        4999 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.hs-cgtpf03
VERSION     urn.local.jonasblomme.hs-cgtpf03
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..1801
                     /modified_by="jonasblomme"
                     /label="pUM056_0044"
     intron          1162..1658
                     /label="RbcsI"
     CDS             1836..2210
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /modified_by="mamee"
     terminator      2211..2599
                     /regulatory_class="terminator"
                     /note="Ulva mutabilis RbcS terminator"
                     /label="UmtRbcS"
     Linker          2604..2652
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2689..2709)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2770..2820
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2821..3630
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3777..4365
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4964..4984
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cggaagagtc ggaacgacat taatcaatag ctgcagttga tggctggggt accagcagct
       61 gcagttgatg gctggggtgc accgcagctc caggtcgagg tccggtaccg gtgtcggggg
      121 ctgtcgcggt tgtgggcatg ccccgtgaag ccagtaagta tggcttccgt cccgcaagag
      181 cgagcatgtc caccgaaaat tgcttcacgt ggcatgaccc atagcgagtg cgccgtagct
      241 ccagagtggt gctctaagta accagtcgtg gggtcagcag gacatgtaca tatgtccgga
      301 cacagcacgc gcaggattgg aaacatcacc ccacgccaat ttcatctaca cagttctgat
      361 gtggtaaact gtcacgactg ctgcagcgtc gctcgatctc atgatcactg ccccttgacg
      421 cagatgagag tcacgatgac aatctgtacc agtgataagt ccaaaattag caagtaggta
      481 ctgcgtggtg tccgtttggt cgtgttcgca ttcctgcccg tgcatgcgcc cgcatcaatt
      541 tcgcccgcaa cgtacagagt ggtcactctg acagctatag gggggaaatg gtcacccggg
      601 tggccatgcg ggcaaaagtc gttcatgagt gttgcgacca ctgcccgagc gctgggcacg
      661 tgattggtat ttcacttgag ctgacattcc gcaacattcc attgcgctag agacatgcag
      721 gtttacgcgt gccagtggca tggcatgggg aagtcgagct gtgcgcagct gcaggggctg
      781 gattcgcgtt gggcccaatt gggctcggag gccaaatgat tgttttttgg tgcattgcca
      841 tgcatcgttc ctctatgttc taccaccttg tggagcatac atctgtaacg tatctcatga
      901 attgggctca ccggccgcgc gtggctctga tgcaacatat ggcgatcgac caatatcggt
      961 ggcgcgcgga tcgcgcgcgc cgagcgcatg caggattcat tcacttgatt attcggggat
     1021 attcgcagtg aaagatccag tggggatact cgttgcccga tcctcacccc cagatacagt
     1081 cgatcccgca cgcatgcgca cgcatggcac cgccagatcg atcggtgcgg atcatgcagg
     1141 attgtcagga tgcaccccag cgtgcgcatc gcgtgtcaat gctggcgctg ctgacgtccg
     1201 ctgcctaaac tgcatgaaag ctcctgcagt cgcaagtagt ccttttaact tgttgtatcg
     1261 gaacaaatgg ttgtagtctg tgccgtgtga cgatcttgag ccgcctggtc ggcaggtcca
     1321 atgtcggcct ggaccctgca caccctgccc accctgccgc tgctcacccg ttcacgaccc
     1381 cacaggtgct cgcagcagta attgtcgata gatagcaact gtgttctgac tattctttga
     1441 ccttccgcat ttgggactgc ggcggcacac ctactatccg caatgcacac cctggtgctg
     1501 gtactgcctg cctttccgcg cgagcccaca ccatgcaggc gcatagtaat ggactgtgat
     1561 tgatttaggc aactgtatga aacctcttca tcgtgccttg gttgcttgcg ataccctcac
     1621 gtgagttcat atgaccaacg ctggcacgac ccctgcagag gatcgcgtgt aggcatggct
     1681 gcttggtcca aaaaacgaca gcatgcccgt gtgtctgcct gagtgcatat atgcagctgc
     1741 cgtccgcaca atccttacca gaaccaaact tcgccgccca catcgaacag cactagtaac
     1801 aaacagtatt cagtcgactg gtaccaacag gctccatggc caagatcacc agcgccgttc
     1861 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     1921 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     1981 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2041 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2101 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2161 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactaa gcagtcaggc
     2221 agagtcgttg ttgggtgtat ggttgcacaa agctagacct cgagctggtc ggttagaaag
     2281 agatgcttgt gcgactggat gtaacaggca caatccgagg aaaatgcaac agaaacgtta
     2341 tgatgtaaca tctgagcaca gtcaataaaa ttgcatgttg tatgccgcac acaaaagtgt
     2401 tgttaaacat gcgatgtggt tgtcaaatca ttattcgcac aaccatggac cgaacctctg
     2461 gcacgctgca tacgctgtat tcattcacat catgttttct acttgtcaca tgctctgcac
     2521 caagtaactc cgattagtta ggttcgcaca tgtcatcgga gtggaccact tcgaatcatt
     2581 tggttcgaag ataaaccggt cagtaagcta ggtagtaact agtgcgactg tctagaatcg
     2641 cgtatgcgag tgtatgagct cgatatcccc tatagtgagt cgtattacat ggtcatagct
     2701 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2761 ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2821 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2881 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2941 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     3001 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     3061 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     3121 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     3181 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     3241 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     3301 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     3361 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     3421 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     3481 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     3541 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3601 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3661 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3721 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4441 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4501 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4561 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4621 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4681 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4741 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4801 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4861 gaaaggccca gacttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4921 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4981 cttaagctcg ggcccacct
//
