LOCUS       pGG-pUM140_0016-BleR-F2A-YFP-PmMalateSynthase-UmtRbcS        6530 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.j2-cgtpsou
VERSION     urn.local.jonasblomme.j2-cgtpsou
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1934
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1939..1946,2444..2807)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          1947..2443
                     /label="RbcsI"
     CDS             2808..2882
                     /label="F2A"
     CDS             2922..3638
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     CDS             3645..3677
                     /created_by="jonasblomme"
                     /modified_by="jonasblomme"
                     /label="Pumpkin malate synthase Signal"
     Linker          3682..3730
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     terminator      3742..4130
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          4135..4183
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(4220..4240)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4301..4351
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4352..5161
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5308..5896
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6495..6515
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
       61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
      121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
      181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
      241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
      301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
      361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
      421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
      481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
      541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
      601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
      661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
      721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
      781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
      841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
      901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
      961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
     1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
     1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
     1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
     1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
     1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
     1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
     1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
     1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
     1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
     1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
     1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
     1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
     1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
     1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
     1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
     1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
     1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
     2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
     2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
     2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
     2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
     2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
     2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
     2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
     2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga
     2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc
     2881 ccggctcaac agtattcagt cgactggtac caacaggctc catggtgagc aagggcgagg
     2941 agctgttcac gggcgtggtg cccatcctgg tggagctgga cggcgacgtg aacggccaca
     3001 agttcagcgt gtccggcgag ggcgagggcg acgccaccta cggcaagctg accctgaagt
     3061 tcatctgcac gaccggcaag ctgcccgtgc cctggcccac cctggtgacc accttcggct
     3121 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaaga
     3181 gcgccatgcc ggagggctac gtgcaggagc gcaccatctt cttcaaggac gacggcaact
     3241 acaagacccg cgcggaggtg aagttcgagg gcgacacgct ggtgaaccgc atcgagctga
     3301 agggcatcga cttcaaggag gacggcaaca tcctgggcca caagctggag tacaactaca
     3361 acagccacaa cgtgtacatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3421 agatccgcca caacatcgag gacggctcgg tgcagctggc ggaccactac cagcagaaca
     3481 cccccatcgg cgacggcccg gtgctgctgc ccgacaacca ctacctgtcc taccagagcg
     3541 ccctgtccaa ggaccccaac gagaagcgcg accacatggt gctgctggag ttcgtgaccg
     3601 ccgccggcat caccctgggc atggacgagc tgtacaagtc agctatccac cacccgcgcg
     3661 agctgagccg cctgtgactg ctaagctagg tagtaactag tgcgactgtc tagaatcgcg
     3721 tatgcgagtg tatctgactg cgcagtcagg cagagtcgtt gttgggtgta tggttgcaca
     3781 aaactagacc tcgagctggt cggttagaaa gagatgcttg tgcgactgga tgtaacaggc
     3841 acaatccgag gaaaatgcaa cagaaacgtt atgatgtaac atctgagcac agtcaataaa
     3901 attgcatgtt gtatgccgca cacaaaagtg ttgttaaaca tgcgatgtgg ttgtcaaatc
     3961 attattcgca caaccatgga ccgaacctct ggcacgctgc atacgctgta ttcattcaca
     4021 tcatgttttc tacttgtcac atgctctgca ccaagtaact ccgattagtt aggttcgcac
     4081 atgtcatcgg agtggaccac ttcgaatcat ttggttcgaa gataaaccgg actataagct
     4141 aggtagtaac tagtgcgact gtctagaatc gcgtatgcga gtgtatgagc tcgatatccc
     4201 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     4261 aaatctctga tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc
     4321 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     4381 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     4441 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     4501 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     4561 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     4621 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     4681 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     4741 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     4801 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     4861 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     4921 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     4981 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     5041 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     5101 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     5161 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     5221 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     5281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6121 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6181 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
     6241 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     6301 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     6361 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agacttccga ctgagccttt
     6421 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
     6481 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggcccacct
//
