LOCUS       pGG-pUM140_0016-BleR-F2A-CreYFP-SV40-UmtRbcS        6467 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.j0-cgtpsou
VERSION     urn.local.jonasblomme.j0-cgtpsou
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1934
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1939..1946,2444..2807)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          1947..2443
                     /label="RbcsI"
     CDS             2808..2882
                     /label="F2A"
     Linker          2883..2912
                     /origin="C-linker-D (53bp linear)Fragment: from BsaI cut
                     at 12^13 to BsaI cut at 40^41, 28bpOrientation:
                     originalPlus strand 5': no changePlus strand 3': no
                     changeMinus strand 5': no changeMinus strand 3': no
                     change"
     CDS             2919..3635
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     NLS             3651..3671
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     terminator      3679..4067
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          4072..4120
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(4157..4177)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4238..4288
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4289..5098
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5245..5833
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6432..6452
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
       61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
      121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
      181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
      241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
      301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
      361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
      421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
      481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
      541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
      601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
      661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
      721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
      781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
      841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
      901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
      961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
     1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
     1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
     1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
     1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
     1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
     1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
     1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
     1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
     1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
     1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
     1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
     1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
     1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
     1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
     1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
     1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
     1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
     2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
     2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
     2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
     2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
     2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
     2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
     2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
     2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga
     2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc
     2881 ccggctccgt attcagtcga ctggtaccaa catcagctat ggtgagcaag ggcgaggagc
     2941 tgttcacggg cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt
     3001 tcagcgtgtc cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagttca
     3061 tctgcacgac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ttcggctacg
     3121 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagagcg
     3181 ccatgccgga gggctacgtg caggagcgca ccatcttctt caaggacgac ggcaactaca
     3241 agacccgcgc ggaggtgaag ttcgagggcg acacgctggt gaaccgcatc gagctgaagg
     3301 gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
     3361 gccacaacgt gtacatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3421 tccgccacaa catcgaggac ggctcggtgc agctggcgga ccactaccag cagaacaccc
     3481 ccatcggcga cggcccggtg ctgctgcccg acaaccacta cctgtcctac cagagcgccc
     3541 tgtccaagga ccccaacgag aagcgcgacc acatggtgct gctggagttc gtgaccgccg
     3601 ccggcatcac cctgggcatg gacgagctgt acaagatgtc aagggctgat cctaagaaga
     3661 agaggaaggt ttgactgcgc agtcaggcag agtcgttgtt gggtgtatgg ttgcacaaaa
     3721 ctagacctcg agctggtcgg ttagaaagag atgcttgtgc gactggatgt aacaggcaca
     3781 atccgaggaa aatgcaacag aaacgttatg atgtaacatc tgagcacagt caataaaatt
     3841 gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc gatgtggttg tcaaatcatt
     3901 attcgcacaa ccatggaccg aacctctggc acgctgcata cgctgtattc attcacatca
     3961 tgttttctac ttgtcacatg ctctgcacca agtaactccg attagttagg ttcgcacatg
     4021 tcatcggagt ggaccacttc gaatcatttg gttcgaagat aaaccggact ataagctagg
     4081 tagtaactag tgcgactgtc tagaatcgcg tatgcgagtg tatgagctcg atatccccta
     4141 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     4201 tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc
     4261 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     4321 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     4381 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     4441 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     4501 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     4561 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     4621 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     4681 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     4741 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     4801 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     4861 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     4921 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     4981 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     5041 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     5101 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     5161 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     5221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5941 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6001 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6061 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6121 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     6181 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     6241 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     6301 gcgttcaccg acaaacaaca gataaaacga aaggcccaga cttccgactg agcctttcgt
     6361 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     6421 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccacct
//
