LOCUS pGG-pUM140_0016-BleR-F2A-CreYFP-N7b-UmtRbcS 6680 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.iz-cgtpsou
VERSION urn.local.jonasblomme.iz-cgtpsou
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
promoter 1..1934
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(1939..1946,2444..2807)
/gene="ble"
/note="derived from Streptoalloteichus hindustanus,
adapted to Chlamydomonas reinhardtii and Ulva mutabilis
codon usage"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="ABY50194.1"
/db_xref="GI:163962062"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
intron 1947..2443
/label="RbcsI"
CDS 2808..2882
/label="F2A"
Linker 2883..2912
/origin="C-linker-D (53bp linear)Fragment: from BsaI cut
at 12^13 to BsaI cut at 40^41, 28bpOrientation:
originalPlus strand 5': no changePlus strand 3': no
changeMinus strand 5': no changeMinus strand 3': no
change"
CDS 2919..3635
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
/modified_by="jonasblomme"
/label="YFP"
NLS 3636..3884
/created_by="warddecaestecker"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on protein similarity"
/Transferred_From="NLS N7"
/Transferred_Translation_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (NLS N7)"
/label="N7"
terminator 3892..4280
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 4285..4333
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(4370..4390)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 4451..4501
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 4502..5311
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 5458..6046
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 6645..6665
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga
2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc
2881 ccggctccgt attcagtcga ctggtaccaa catcagctat ggtgagcaag ggcgaggagc
2941 tgttcacggg cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt
3001 tcagcgtgtc cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagttca
3061 tctgcacgac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ttcggctacg
3121 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagagcg
3181 ccatgccgga gggctacgtg caggagcgca ccatcttctt caaggacgac ggcaactaca
3241 agacccgcgc ggaggtgaag ttcgagggcg acacgctggt gaaccgcatc gagctgaagg
3301 gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
3361 gccacaacgt gtacatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
3421 tccgccacaa catcgaggac ggctcggtgc agctggcgga ccactaccag cagaacaccc
3481 ccatcggcga cggcccggtg ctgctgcccg acaaccacta cctgtcctac cagagcgccc
3541 tgtccaagga ccccaacgag aagcgcgacc acatggtgct gctggagttc gtgaccgccg
3601 ccggcatcac cctgggcatg gacgagctgt acaaggcggc cgcatctgaa ttcaagcgtg
3661 aagagcaagc aaggaaagct aaggtgaaca atgagaaaaa gacggaaata gtgaaaccag
3721 agagttgtag caatgaagga gatgtcaagg atctgaaaag aaaggactct gaggatggaa
3781 acgagggtga ggaagaagaa gcttcttcga aaccgaaaaa gccaaaagtt gctctttctc
3841 atcttcagga cattgacgac acagaagctg atcaagaaga agagtgactg cgcagtcagg
3901 cagagtcgtt gttgggtgta tggttgcaca aaactagacc tcgagctggt cggttagaaa
3961 gagatgcttg tgcgactgga tgtaacaggc acaatccgag gaaaatgcaa cagaaacgtt
4021 atgatgtaac atctgagcac agtcaataaa attgcatgtt gtatgccgca cacaaaagtg
4081 ttgttaaaca tgcgatgtgg ttgtcaaatc attattcgca caaccatgga ccgaacctct
4141 ggcacgctgc atacgctgta ttcattcaca tcatgttttc tacttgtcac atgctctgca
4201 ccaagtaact ccgattagtt aggttcgcac atgtcatcgg agtggaccac ttcgaatcat
4261 ttggttcgaa gataaaccgg actataagct aggtagtaac tagtgcgact gtctagaatc
4321 gcgtatgcga gtgtatgagc tcgatatccc ctatagtgag tcgtattaca tggtcatagc
4381 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
4441 aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
4501 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
4561 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
4621 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
4681 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
4741 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
4801 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
4861 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
4921 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
4981 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
5041 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
5101 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
5161 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
5221 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
5281 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
5341 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
5401 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
5581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
5641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
5701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
5761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
5821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
5881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
5941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
6121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
6181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
6241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
6301 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
6361 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
6421 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
6481 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
6541 cgaaaggccc agacttccga ctgagccttt cgttttattt gatgcctggc agttccctac
6601 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
6661 tcttaagctc gggcccacct
//