LOCUS pGG-pUM140_0016-BleR-F2A-CreYFP-N7b-UmtRbcS 6680 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.iz-cgtpsou VERSION urn.local.jonasblomme.iz-cgtpsou KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..1934 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(1939..1946,2444..2807) /gene="ble" /note="derived from Streptoalloteichus hindustanus, adapted to Chlamydomonas reinhardtii and Ulva mutabilis codon usage" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein" /protein_id="ABY50194.1" /db_xref="GI:163962062" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQD" intron 1947..2443 /label="RbcsI" CDS 2808..2882 /label="F2A" Linker 2883..2912 /origin="C-linker-D (53bp linear)Fragment: from BsaI cut at 12^13 to BsaI cut at 40^41, 28bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" CDS 2919..3635 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /modified_by="jonasblomme" /label="YFP" NLS 3636..3884 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on protein similarity" /Transferred_From="NLS N7" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (NLS N7)" /label="N7" terminator 3892..4280 /regulatory_class="terminator" /note="Ulva RbcS terminator" /label="UmtRbcS" Linker 4285..4333 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(4370..4390) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 4451..4501 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 4502..5311 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 5458..6046 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 6645..6665 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag 61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc 121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg 181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc 241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag 301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg 361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg 421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta 481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa 541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg 601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg 661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg 721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc 781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg 841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga 901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt 961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc 1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat 1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga 1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga 1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt 1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt 1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg 1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct 1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta 1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg 1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca 1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc 1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac 1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt 1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc 1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct 1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac 1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg 2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag 2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac 2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc 2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg 2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact 2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc 2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc 2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga 2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc 2881 ccggctccgt attcagtcga ctggtaccaa catcagctat ggtgagcaag ggcgaggagc 2941 tgttcacggg cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt 3001 tcagcgtgtc cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagttca 3061 tctgcacgac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ttcggctacg 3121 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagagcg 3181 ccatgccgga gggctacgtg caggagcgca ccatcttctt caaggacgac ggcaactaca 3241 agacccgcgc ggaggtgaag ttcgagggcg acacgctggt gaaccgcatc gagctgaagg 3301 gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca 3361 gccacaacgt gtacatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 3421 tccgccacaa catcgaggac ggctcggtgc agctggcgga ccactaccag cagaacaccc 3481 ccatcggcga cggcccggtg ctgctgcccg acaaccacta cctgtcctac cagagcgccc 3541 tgtccaagga ccccaacgag aagcgcgacc acatggtgct gctggagttc gtgaccgccg 3601 ccggcatcac cctgggcatg gacgagctgt acaaggcggc cgcatctgaa ttcaagcgtg 3661 aagagcaagc aaggaaagct aaggtgaaca atgagaaaaa gacggaaata gtgaaaccag 3721 agagttgtag caatgaagga gatgtcaagg atctgaaaag aaaggactct gaggatggaa 3781 acgagggtga ggaagaagaa gcttcttcga aaccgaaaaa gccaaaagtt gctctttctc 3841 atcttcagga cattgacgac acagaagctg atcaagaaga agagtgactg cgcagtcagg 3901 cagagtcgtt gttgggtgta tggttgcaca aaactagacc tcgagctggt cggttagaaa 3961 gagatgcttg tgcgactgga tgtaacaggc acaatccgag gaaaatgcaa cagaaacgtt 4021 atgatgtaac atctgagcac agtcaataaa attgcatgtt gtatgccgca cacaaaagtg 4081 ttgttaaaca tgcgatgtgg ttgtcaaatc attattcgca caaccatgga ccgaacctct 4141 ggcacgctgc atacgctgta ttcattcaca tcatgttttc tacttgtcac atgctctgca 4201 ccaagtaact ccgattagtt aggttcgcac atgtcatcgg agtggaccac ttcgaatcat 4261 ttggttcgaa gataaaccgg actataagct aggtagtaac tagtgcgact gtctagaatc 4321 gcgtatgcga gtgtatgagc tcgatatccc ctatagtgag tcgtattaca tggtcatagc 4381 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 4441 aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 4501 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 4561 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 4621 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 4681 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 4741 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 4801 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 4861 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 4921 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 4981 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 5041 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 5101 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 5161 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 5221 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 5281 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg 5341 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt 5401 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 5461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 5521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 5581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 5641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 5701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 5761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 5821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 5881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 5941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 6001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 6061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 6121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 6181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 6241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 6301 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga 6361 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg 6421 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa 6481 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa 6541 cgaaaggccc agacttccga ctgagccttt cgttttattt gatgcctggc agttccctac 6601 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag 6661 tcttaagctc gggcccacct //