LOCUS pGG-pUM140_0016-BleR-F2A-BIP-YFPHDEL-UmtRbcS 6647 bp DNA circular UNA 12-APR-2023
DEFINITION .
ACCESSION urn.local.jonasblomme.iv-cgtpsot
VERSION urn.local.jonasblomme.iv-cgtpsot
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
promoter 1..1934
/modified_by="jonasblomme"
/label="pUM140_0016"
CDS join(1939..1946,2444..2807)
/gene="ble"
/note="derived from Streptoalloteichus hindustanus,
adapted to Chlamydomonas reinhardtii and Ulva mutabilis
codon usage"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="ABY50194.1"
/db_xref="GI:163962062"
/translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
intron 1947..2443
/label="RbcsI"
CDS 2808..2882
/label="F2A"
CDS 2892..3059
/modified_by="jonasblomme"
/label="BIP"
CDS 3066..3782
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
/modified_by="jonasblomme"
/label="YFP"
CDS 3783..3794
/created_by="jonasblomme"
/label="HDEL"
Linker 3799..3847
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
terminator 3859..4247
/regulatory_class="terminator"
/note="Ulva RbcS terminator"
/label="UmtRbcS"
Linker 4252..4300
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
primer complement(4337..4357)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13R"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
(pCDB-Cas9 - GWM13R)"
/label="GWM13R"
promoter 4418..4468
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 4469..5278
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 5425..6013
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 6612..6632
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCDB-Cas9 - GWM13F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
(pCDB-Cas9 - GWM13F)"
/label="GWM13F"
ORIGIN
1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga
2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc
2881 ccggctcaac aatggcgcag tggaaggctg ctgtgctgtt gctgggtagg tccgcgcgct
2941 gcgtcccgtc gcgctggatc tgtcatctaa ttataatgtt ttgctcgcag cgctggcctg
3001 cgcctcttac ggcttcggcg tttgggctga ggaggagaag ctcggcaccg ttattggtcg
3061 gctccatggt gagcaagggc gaggagctgt tcacgggcgt ggtgcccatc ctggtggagc
3121 tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgacgcca
3181 cctacggcaa gctgaccctg aagttcatct gcacgaccgg caagctgccc gtgccctggc
3241 ccaccctggt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
3301 tgaagcagca cgacttcttc aagagcgcca tgccggaggg ctacgtgcag gagcgcacca
3361 tcttcttcaa ggacgacggc aactacaaga cccgcgcgga ggtgaagttc gagggcgaca
3421 cgctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
3481 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
3541 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc tcggtgcagc
3601 tggcggacca ctaccagcag aacaccccca tcggcgacgg cccggtgctg ctgcccgaca
3661 accactacct gtcctaccag agcgccctgt ccaaggaccc caacgagaag cgcgaccaca
3721 tggtgctgct ggagttcgtg accgccgccg gcatcaccct gggcatggac gagctgtaca
3781 agcacgacga gctgtcagta agctaggtag taactagtgc gactgtctag aatcgcgtat
3841 gcgagtgtat ctgactgcgc agtcaggcag agtcgttgtt gggtgtatgg ttgcacaaaa
3901 ctagacctcg agctggtcgg ttagaaagag atgcttgtgc gactggatgt aacaggcaca
3961 atccgaggaa aatgcaacag aaacgttatg atgtaacatc tgagcacagt caataaaatt
4021 gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc gatgtggttg tcaaatcatt
4081 attcgcacaa ccatggaccg aacctctggc acgctgcata cgctgtattc attcacatca
4141 tgttttctac ttgtcacatg ctctgcacca agtaactccg attagttagg ttcgcacatg
4201 tcatcggagt ggaccacttc gaatcatttg gttcgaagat aaaccggact ataagctagg
4261 tagtaactag tgcgactgtc tagaatcgcg tatgcgagtg tatgagctcg atatccccta
4321 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
4381 tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc
4441 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
4501 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
4561 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
4621 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
4681 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
4741 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
4801 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
4861 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
4921 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
4981 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
5041 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
5101 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
5161 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
5221 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
5281 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
5341 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
6121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
6181 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
6241 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
6301 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
6361 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
6421 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
6481 gcgttcaccg acaaacaaca gataaaacga aaggcccaga cttccgactg agcctttcgt
6541 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
6601 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccacct
//