LOCUS pGG-pUM140_0016-BleR-F2A-BIP-YFPHDEL-UmtRbcS 6647 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.iv-cgtpsot VERSION urn.local.jonasblomme.iv-cgtpsot KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..1934 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(1939..1946,2444..2807) /gene="ble" /note="derived from Streptoalloteichus hindustanus, adapted to Chlamydomonas reinhardtii and Ulva mutabilis codon usage" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein" /protein_id="ABY50194.1" /db_xref="GI:163962062" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQD" intron 1947..2443 /label="RbcsI" CDS 2808..2882 /label="F2A" CDS 2892..3059 /modified_by="jonasblomme" /label="BIP" CDS 3066..3782 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /modified_by="jonasblomme" /label="YFP" CDS 3783..3794 /created_by="jonasblomme" /label="HDEL" Linker 3799..3847 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" terminator 3859..4247 /regulatory_class="terminator" /note="Ulva RbcS terminator" /label="UmtRbcS" Linker 4252..4300 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(4337..4357) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 4418..4468 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 4469..5278 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 5425..6013 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 6612..6632 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag 61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc 121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg 181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc 241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag 301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg 361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg 421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta 481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa 541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg 601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg 661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg 721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc 781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg 841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga 901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt 961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc 1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat 1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga 1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga 1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt 1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt 1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg 1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct 1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta 1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg 1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca 1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc 1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac 1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt 1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc 1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct 1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac 1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg 2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag 2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac 2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc 2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg 2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact 2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc 2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc 2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga 2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc 2881 ccggctcaac aatggcgcag tggaaggctg ctgtgctgtt gctgggtagg tccgcgcgct 2941 gcgtcccgtc gcgctggatc tgtcatctaa ttataatgtt ttgctcgcag cgctggcctg 3001 cgcctcttac ggcttcggcg tttgggctga ggaggagaag ctcggcaccg ttattggtcg 3061 gctccatggt gagcaagggc gaggagctgt tcacgggcgt ggtgcccatc ctggtggagc 3121 tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgacgcca 3181 cctacggcaa gctgaccctg aagttcatct gcacgaccgg caagctgccc gtgccctggc 3241 ccaccctggt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca 3301 tgaagcagca cgacttcttc aagagcgcca tgccggaggg ctacgtgcag gagcgcacca 3361 tcttcttcaa ggacgacggc aactacaaga cccgcgcgga ggtgaagttc gagggcgaca 3421 cgctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 3481 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga 3541 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc tcggtgcagc 3601 tggcggacca ctaccagcag aacaccccca tcggcgacgg cccggtgctg ctgcccgaca 3661 accactacct gtcctaccag agcgccctgt ccaaggaccc caacgagaag cgcgaccaca 3721 tggtgctgct ggagttcgtg accgccgccg gcatcaccct gggcatggac gagctgtaca 3781 agcacgacga gctgtcagta agctaggtag taactagtgc gactgtctag aatcgcgtat 3841 gcgagtgtat ctgactgcgc agtcaggcag agtcgttgtt gggtgtatgg ttgcacaaaa 3901 ctagacctcg agctggtcgg ttagaaagag atgcttgtgc gactggatgt aacaggcaca 3961 atccgaggaa aatgcaacag aaacgttatg atgtaacatc tgagcacagt caataaaatt 4021 gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc gatgtggttg tcaaatcatt 4081 attcgcacaa ccatggaccg aacctctggc acgctgcata cgctgtattc attcacatca 4141 tgttttctac ttgtcacatg ctctgcacca agtaactccg attagttagg ttcgcacatg 4201 tcatcggagt ggaccacttc gaatcatttg gttcgaagat aaaccggact ataagctagg 4261 tagtaactag tgcgactgtc tagaatcgcg tatgcgagtg tatgagctcg atatccccta 4321 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa 4381 tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc 4441 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc 4501 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt 4561 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt 4621 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa 4681 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga 4741 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata 4801 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc 4861 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca 4921 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg 4981 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt 5041 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg 5101 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg 5161 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat 5221 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc 5281 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc 5341 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc 5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 6061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 6121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 6181 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 6241 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 6301 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg 6361 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct 6421 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga 6481 gcgttcaccg acaaacaaca gataaaacga aaggcccaga cttccgactg agcctttcgt 6541 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca 6601 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccacct //