LOCUS       pGG-pUM140_0016-BleR-F2A-atpA-YFP-UmtRbcS        6869 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.iu-cgtpsot
VERSION     urn.local.jonasblomme.iu-cgtpsot
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1934
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1939..1946,2444..2807)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          1947..2443
                     /label="RbcsI"
     CDS             2808..2882
                     /label="F2A"
     CDS             join(2892..2925,3023..3056,3139..3178,3261..3293)
                     /SerialCloner_Color="&h0D8E8C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="jonasblomme"
                     /label="atpA MTS with introns"
     intron          2926..3022
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          3057..3138
                     /created_by="jonasblomme"
                     /label="Intron"
     intron          3179..3260
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             3300..4016
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     Linker          4021..4069
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     terminator      4081..4469
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          4474..4522
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(4559..4579)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4640..4690
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4691..5500
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5647..6235
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6834..6854
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag
       61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc
      121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg
      181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc
      241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag
      301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg
      361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg
      421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta
      481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa
      541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg
      601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg
      661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg
      721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc
      781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg
      841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga
      901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt
      961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc
     1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat
     1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga
     1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga
     1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt
     1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt
     1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg
     1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct
     1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta
     1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg
     1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca
     1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc
     1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac
     1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt
     1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc
     1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct
     1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac
     1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg
     2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag
     2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac
     2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc
     2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg
     2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact
     2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc
     2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc
     2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggcgtga
     2821 agcagaccct gaacttcgac ctgctgaagc tggcgggcga cgtggagagc aacccgggcc
     2881 ccggctcaac aatgcgcagc caggcgcttt ctcttgcccg cgcgggtgag tccgggacaa
     2941 ggtggcgtcg cgggtgtgcg cgcatggtcg gccggtgtcg tgactcggag cttgccccgc
     3001 cgcaatgccc tcgcttttgc aggcctgctg cagctgagct cccagacggg tgccaggtaa
     3061 gattgccggg tccatgcggg ctatatatgc tccgaaatcg atgcacttac acgtgcggac
     3121 aatggtgttg ctctgcagcc tggagggtgg cttcgcgctg tccaagcgtg ccgagcaggt
     3181 aagaagcttg atacgagcaa tggaaggggc tgcatgtgcg gtgccggaag cgctaatcgg
     3241 atgtcgctgt tttccggtag gcgctcatcc gcgccagccg cgcgttcgcc agcggctcca
     3301 tggtgagcaa gggcgaggag ctgttcacgg gcgtggtgcc catcctggtg gagctggacg
     3361 gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgac gccacctacg
     3421 gcaagctgac cctgaagttc atctgcacga ccggcaagct gcccgtgccc tggcccaccc
     3481 tggtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     3541 agcacgactt cttcaagagc gccatgccgg agggctacgt gcaggagcgc accatcttct
     3601 tcaaggacga cggcaactac aagacccgcg cggaggtgaa gttcgagggc gacacgctgg
     3661 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctgggccaca
     3721 agctggagta caactacaac agccacaacg tgtacatcat ggccgacaag cagaagaacg
     3781 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggctcggtg cagctggcgg
     3841 accactacca gcagaacacc cccatcggcg acggcccggt gctgctgccc gacaaccact
     3901 acctgtccta ccagagcgcc ctgtccaagg accccaacga gaagcgcgac cacatggtgc
     3961 tgctggagtt cgtgaccgcc gccggcatca ccctgggcat ggacgagctg tacaagtcag
     4021 taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt atctgactgc
     4081 gcagtcaggc agagtcgttg ttgggtgtat ggttgcacaa aactagacct cgagctggtc
     4141 ggttagaaag agatgcttgt gcgactggat gtaacaggca caatccgagg aaaatgcaac
     4201 agaaacgtta tgatgtaaca tctgagcaca gtcaataaaa ttgcatgttg tatgccgcac
     4261 acaaaagtgt tgttaaacat gcgatgtggt tgtcaaatca ttattcgcac aaccatggac
     4321 cgaacctctg gcacgctgca tacgctgtat tcattcacat catgttttct acttgtcaca
     4381 tgctctgcac caagtaactc cgattagtta ggttcgcaca tgtcatcgga gtggaccact
     4441 tcgaatcatt tggttcgaag ataaaccgga ctataagcta ggtagtaact agtgcgactg
     4501 tctagaatcg cgtatgcgag tgtatgagct cgatatcccc tatagtgagt cgtattacat
     4561 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     4621 acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     4681 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     4741 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     4801 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     4861 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     4921 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     4981 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     5041 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     5101 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     5161 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     5221 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     5281 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     5341 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     5401 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     5461 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     5521 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     5581 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5761 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6361 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6421 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6481 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
     6541 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
     6601 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     6661 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     6721 cagataaaac gaaaggccca gacttccgac tgagcctttc gttttatttg atgcctggca
     6781 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
     6841 gacggccagt cttaagctcg ggcccacct
//
