LOCUS       pGG-UmpRbcS-BleR-T2A-mTagBFP-UmtRbcS        5013 bp    DNA     circular UNA 11-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.pl-cgtpvim
VERSION     urn.local.jonasblomme.pl-cgtpvim
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     promoter        1..546
                     /regulatory_class="promoter"
                     /note="Ulva RbcS promoter"
                     /label="UmpRbcS"
     CDS             join(551..558,1056..1419)
                     /gene="ble"
                     /note="derived from Streptoalloteichus hindustanus,
                     adapted to Chlamydomonas reinhardtii and Ulva mutabilis
                     codon usage"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="ABY50194.1"
                     /db_xref="GI:163962062"
                     /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     intron          559..1055
                     /label="RbcsI"
     CDS             1420..1482
                     /modified_by="jonasblomme"
                     /label="T2A"
     Linker          1483..1512
                     /origin="C-linker-D (53bp linear)Fragment: from BsaI cut
                     at 12^13 to BsaI cut at 40^41, 28bpOrientation:
                     originalPlus strand 5': no changePlus strand 3': no
                     changeMinus strand 5': no changeMinus strand 3': no
                     change"
     CDS             1519..2217
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mTagBFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; mTagBFP
                     (<a
                     href=""urn:local:jonasblomme:2d-cnsqc2r"">mTagBFP2</a>)"
                     /modified_by="jonasblomme"
                     /label="mTagBFP"
     terminator      2225..2613
                     /regulatory_class="terminator"
                     /note="Ulva RbcS terminator"
                     /label="UmtRbcS"
     Linker          2618..2666
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2703..2723)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2784..2834
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2835..3644
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3791..4379
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4978..4998
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacaaca atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg
      601 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa
      661 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg
      721 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac
      781 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct
      841 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta
      901 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga
      961 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg
     1021 agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt tccggtgctc
     1081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     1141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     1201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     1261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     1321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     1381 gccggcaact gcgtgcactt cgtggccgag gagcaggacg gcagcggcga gggcagagga
     1441 agtcttctaa catgcggtga cgtggaggag aatccgggcc ctggctccgt attcagtcga
     1501 ctggtaccaa catcagctat gagcgagctg atcaaggaga acatgcacat gaagctgtac
     1561 atggagggca ccgtggacaa ccaccacttc aagtgcacca gcgagggcga gggcaagccc
     1621 tacgagggca cccagaccat gcgcatcaag gtggtggagg gcggccccct gcccttcgcc
     1681 ttcgacatcc tggccacctc gttcctgtac ggcagcaaga ccttcatcaa ccacacccag
     1741 ggcatccccg acttcttcaa gcagagcttc cccgagggct tcacctggga gcgcgtgacc
     1801 acctacgagg acggcggcgt gctgaccgcc acccaggaca ccagcctcca ggacggctgc
     1861 ctgatctaca acgtgaagat ccgcggcgtg aacttcacca gcaacggccc cgtgatgcag
     1921 aagaagaccc tgggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
     1981 gagggccgca acgacatggc cctgaagctg gtgggcggca gccacctgat cgccaacatc
     2041 aagaccacct accgcagcaa gaagcccgcc aagaacctga agatgcccgg cgtgtactac
     2101 gtggactacc gcctggagcg catcaaggag gccaacaacg agacgtacgt ggagcagcac
     2161 gaggtggccg tggcccgcta ctgcgacctg cccagcaagc tgggccacaa gctgaactaa
     2221 ctgcgcagtc aggcagagtc gttgttgggt gtatggttgc acaaaactag acctcgagct
     2281 ggtcggttag aaagagatgc ttgtgcgact ggatgtaaca ggcacaatcc gaggaaaatg
     2341 caacagaaac gttatgatgt aacatctgag cacagtcaat aaaattgcat gttgtatgcc
     2401 gcacacaaaa gtgttgttaa acatgcgatg tggttgtcaa atcattattc gcacaaccat
     2461 ggaccgaacc tctggcacgc tgcatacgct gtattcattc acatcatgtt ttctacttgt
     2521 cacatgctct gcaccaagta actccgatta gttaggttcg cacatgtcat cggagtggac
     2581 cacttcgaat catttggttc gaagataaac cggactataa gctaggtagt aactagtgcg
     2641 actgtctaga atcgcgtatg cgagtgtatg agctcgatat cccctatagt gagtcgtatt
     2701 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2761 ttgcacaaga taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta
     2821 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     2881 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2941 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     3001 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     3061 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     3121 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     3181 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     3241 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     3301 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     3361 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     3421 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     3481 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     3541 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     3601 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     3661 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     3721 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4621 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4681 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4741 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4801 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4861 acaacagata aaacgaaagg cccagacttc cgactgagcc tttcgtttta tttgatgcct
     4921 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     4981 aaacgacggc cagtcttaag ctcgggccca cct
//
